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671.
Manju Sharma Rohit Soni Asiya Nazir Harinder Singh Oberoi Bhupinder Singh Chadha 《Applied biochemistry and biotechnology》2011,163(5):577-591
A thermotolerant Aspergillus fumigatus strain isolated from composting pile of mixed industrial waste was found to produce a spectrum of cellulase and hemicellulases
when cultured on rice straw solidified substrate. The two-dimensional electrophoresis (2DE) resolved the secretome into 57
distinct protein spots. The zymograms developed against 2DE gels identified the presence of three β-glucosidases and five
CBHI/EGI isoforms in the secretome. The peptide mass fingerprinting of 17 protein spots by liquid chromatography mass spectrometry
characterized the secretome into different glycosyl hydrolase families. The enzyme cocktail produced by A. fumigatus was capable of efficient hydrolysis of alkali pretreated rice straw (at 7% and 10% w/v) resulting in 95% and 91% saccharification, respectively. 相似文献
672.
Arvind Sharma Rohit Dhir R. C. Verma 《The European Physical Journal C - Particles and Fields》2011,71(1):1-7
In this paper we study one-dimensional static self-dual non-topological solitons in an Abelian-Higgs Chern–Simons model with a non-canonical kinetic term. We choose a scalar potential with a single minimum and present an analytical soliton solution which corresponds to an electrically charged lump-like soliton without total momentum and located in a region where the scalar field gets maximum. The solitonic boundary conditions impose a discontinuity on the electric field across the soliton line where, then, there is a linear charge density. 相似文献
673.
Rao Priyashi Patel Rohit Shukla Arpit Parmar Paritosh Rawal Rakesh M. Saraf Meenu Goswami Dweipayan 《Molecular diversity》2022,26(1):309-329
Molecular Diversity - The non-structural protein (nsp)-3 of SARS-CoV2 coronavirus is sought to be an essential target protein which is also named as papain-like protease (PLpro). This protease... 相似文献
674.
Shukla Arpit Parmar Paritosh Kapoor Gitanjali Goswami Dweipayan Jha Chaitanya Kumar Patel Baldev Saraf Meenu 《Molecular diversity》2022,26(1):555-568
Molecular Diversity - Microbes possess a tremendous potential to interact with their surroundings and have continued to shape the future of all life forms existing on earth. Of all the groups of... 相似文献
675.
676.
A systematic variation of shape variables \(\beta \) and \(\gamma \) with N and \(N_\mathrm {p}N_\mathrm {n}\) is studied in the framework of an asymmetric rotor model of Davydov and Filippov for the \(Z=\) 50–82, \(N=\) 82–126 major shell space. The role of the \(Z=64\) subshell in producing smooth systematics has been discussed. The quadrupole moments are extracted after considering both axially symmetric and axially asymmetric nuclei. The correlation of \(\beta \) with \(\gamma \) together with the measured quadrupole moments indicates that \(\gamma \)-rigidity is better observed in nuclei with modest deformation. 相似文献
677.
The single alpha helix (SAH) is a recurring motif in biology. The consensus sequence has a di-block architecture that includes repeats of four consecutive glutamate residues followed by four consecutive lysine residues. Measurements show that the overall helicity of sequences with consensus E4K4 repeats is insensitive to a wide range of pH values. Here, we use the recently introduced q-canonical ensemble, which allows us to decouple measurements of charge state and conformation, to explain the observed insensitivity of SAH helicity to pH. We couple the outputs from separate measurements of charge and conformation with atomistic simulations to derive residue-specific quantifications of preferences for being in an alpha helix and for the ionizable residues to be charged vs. uncharged. We find a clear preference for accommodating uncharged Glu residues within internal positions of SAH-forming sequences. The stabilities of alpha helical conformations increase with the number of E4K4 repeats and so do the numbers of accessible charge states that are compatible with forming conformations of high helical content. There is conformational buffering whereby charge state heterogeneity buffers against large-scale conformational changes thus making the overall helicity insensitive to large changes in pH. Further, the results clearly argue against a single, rod-like alpha helical conformation being the only or even dominant conformation in the ensembles of so-called SAH sequences. 相似文献
678.
Dr. Dylan G. Boucher Emily Carroll Zachary A. Nguyen Dr. Rohit G. Jadhav Prof. Olja Simoska Kevin Beaver Prof. Shelley D. Minteer 《Angewandte Chemie (International ed. in English)》2023,62(46):e202307780
Bioelectrocatalytic synthesis is the conversion of electrical energy into value-added products using biocatalysts. These methods merge the specificity and selectivity of biocatalysis and energy-related electrocatalysis to address challenges in the sustainable synthesis of pharmaceuticals, commodity chemicals, fuels, feedstocks and fertilizers. However, the specialized experimental setups and domain knowledge for bioelectrocatalysis pose a significant barrier to adoption. This review introduces key concepts of bioelectrosynthetic systems. We provide a tutorial on the methods of biocatalyst utilization, the setup of bioelectrosynthetic cells, and the analytical methods for assessing bioelectrocatalysts. Key applications of bioelectrosynthesis in ammonia production and small-molecule synthesis are outlined for both enzymatic and microbial systems. This review serves as a necessary introduction and resource for the non-specialist interested in bioelectrosynthetic research. 相似文献