Synthesis and characterization of a poly(N‐isopropylacrylamide) with β‐cyclodextrin as pendant groups

A novel linear poly(N‐isopropylacrylamide) (PNIPA) with β‐cylodextrin (β‐CD) moiety (PNIPA‐β‐CD) was synthesized by the conjugation of β‐CD carrying amino groups (EDA‐β‐CD) onto PNIPA with epoxy groups (P(NIPA‐co‐GMA), M_n = 3.86 × 10⁴), and the related reaction conditions are investigated. PNIPA‐β‐CD was characterized by means of IR, NMR and UV spectroscopes, element analysis, and differential scanning calorimetry (DSC). The number‐average molecular weight (M_n) and the β‐CD content of the obtained PNIPA‐β‐CD are 4.87 × 10⁴ and 18.8 wt %, respectively. PNIPA‐β‐CD can not only respond to temperature stimuli but also include guest molecules. Lower critical solution temperature (LCST) of aqueous PNIPA‐β‐CD solution is similar to that of PNIPA. The association constant (K_a) for PNIPA‐β‐CD with methyl orange (MO) is 2.4 × 10³ L mol^?1 at pH 1.4, which is comparable to that of EDA‐β‐CD (K_a = 2.9 × 10³ L mol^?1). © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 3516–3524, 2005     

基于有序介孔碳/纳米金/L-半胱氨酸修饰电极测定铜(Ⅱ)

将纳米金(NG)电沉积在有序介孔碳(OMC)修饰玻碳电极表面,并将L-半胱氨酸(L-Cys)自组装至OM C/NG修饰电极表面,制备了OM C/NG/L-Cys修饰电极。采用透射电子显微镜考察了OM C的结构,扫描电子显微镜研究了OMC/NG/L-Cys修饰电极的表面形貌。考察了Cu2+在该修饰电极上的电化学行为,优化了Cu2+的测定条件。在最优条件下,溶出峰峰电流与Cu2+浓度在0.05~6.0μmol/L范围内呈良好线性关系,检出限为0.03μmol/L(S/N=3)。方法用于河水样品分析,其加标回收率在92.7%~101.6%之间。     

EXPRESSION OF A PARTIAL SYNTHETIC HUMAN TNF cDNA IN E. coli

A recombinant human tumour necrosis factor (rhTNF) cDNA was constructed. The TNF gene was isolated from a human genomic gene library. There are four exons in the TNF gene. The fourth exou codes for 140 amino acids of the TNF matured protein which is composed of 157 amino acids. A major portion of the fourth exon was isolated and then ligated to a synthesized DNA fragment coding for the remaining amino acids. The partial synthetic hTNF (rhTNF) cDNA thus generated was subcloned into a vector and successfully expressed in E. coli. 5-1 fer1entator was used to produce rhTNF. About 20g (wet weight) of bacterial pellet per liter medium and 106—10~7 units of cytotoxicity to L929 cells per milliliter medium were obtained. rhTNF was purified by HPLC and dried with a freeze dryer, rhTNF with a purity of about 95% in the form of white powder was obtained. The sequence of ten amino acids at the amino terminus of the rhTNF was determined. The result showed that it was identical with that of the natural human TNF.     

溶胶浸渍模板法制备有序多孔氧化钛、氧化锌薄膜

采用溶胶浸渍模板法制备了有序多孔的氧化钛(TiO₂)和氧化锌(ZnO)薄膜。首先，在洁净的玻璃基片上通过浸渍-提拉工艺组装有序的聚苯乙烯微球(PS)阵列模板；然后再采用溶胶浸渍法将TiO₂和ZnO溶胶灌充到PS模板微球的间隙内；最后通过煅烧去除PS而得多孔薄膜。采用SEM观察了薄膜的表面形貌，并用XRD对薄膜的性能进行了表征。结果表明，溶胶的浓度对薄膜形貌有着显著的影响。经煅烧后，TiO₂和ZnO薄膜分别为锐钛矿和六方纤锌矿结构。此外，对模板的组装及溶胶的灌注过程进行了分析。     

Neutron-induced defects in the lithium tetraborate single crystals

The X-band ( x ; 9.4 v GHz) electron spin resonance (ESR) spectra of the un-doped isotopically enriched lithium tetraborate (LTB) Li 2 B 4 O 7 single crystals, irradiated by thermal neutrons (fluences | n =2.74 ‐ 10 15 1 1.79 v ‐ 10 18 v cm m 2 ) were investigated at 300 and 77 v K. The LTB crystals of high chemical purity and optical quality with different isotope compositions ( 6 Li 2 10 B 4 O 7 , 6 Li 2 11 B 4 O 7 , 7 Li 2 10 B 4 O 7 and 7 Li 2 11 B 4 O 7 ) were grown by Czochralski technique. The thermal neutrons (the total quantity >90%) with fluence near 10 18 v cm m 2 induce at least 4 different types of stable paramagnetic centers in the Li and B isotopically enriched LTB crystals. The ESR spectra, electron structure and efficiency of generation for centers, induced by thermal neutrons, essentially depend on neutron fluence and isotope composition of the LTB crystals. The local symmetry and the spin Hamiltonian parameters of the observed paramagnetic centers were determined and their electron structure were established. The possible models and formation mechanism of the radiation defects, induced by thermal neutrons in the LTB lattice, are proposed.     

Fluctuation theory of hydrogen bonding applied to vibration spectra of HOD molecules in liquid water. I. Raman spectra

Statistical distributions of the frequencies of OH vibrations calculated from the experimental Raman spectra of HOD molecules over the temperature range of 10–200 C using the recently developed deconvolution technique have been analysed in the framework of fluctuation theory of hydrogen bonding. Two temperature independent functions that are the basis of the Zhukovsky theory for describing the temperature transformation of frequency distribution functions of OH vibrations in a statistical ensemble of O—H…O bonds were established and analytically approximated. This made it possible to reconstruct the entire set of initial spectra and to extrapolate their forms to a wider temperature range. These results support the continuum model of liquid water structure.     

Calculations of multiquark functions in effective models of strong interaction

In this paper we present our results of the investigation of multiquark equations in the Nambu-Jona-Lasinio model with chiral symmetry of SU(2) group in the mean-field expansion. To formulate the mean-field expansion we have used an iteration scheme of solution of the Schwinger-Dyson equations with the fermion bilocal source. We have considered the equations for Green functions of the Nambu-Jona-Lasinio model up to third step for this iteration scheme. To calculate the high-order corrections to the mean-field approximation, we propose the method of the Legendre transformation with respect to the bilocal source, which allows effectively to take into account the symmetry constraints related with the chiral Ward identity. We discuss also the problem of calculating the multiquark functions in the mean-field expansion for Nambu-Jona-Lasinio-type models with other types of the multifermion sources.     

UH和UH_2分子的结构与势能函数

用相对论有效原子实势 (RECP)和密度泛函 (B3LYP SDD)方法研究了UH ,UH2 基态和低激发态的结构和势能函数 ,导出了分子的光谱数据 .结果表明 ,UH和UH2 的基电子状态分别为X4 Π和X3A2 ,离解能分别为 2 .886eV和5 .2 4 9eV ,UH2 具有C2v对称性 ,得到了UH和UH2 的几个不同的低激发态的结构与光谱数据 .应用多体项展式理论以及数字拟合方法 ,计算得到了UH分子和基态UH2 三原子分子的分析势能函数 ,该函数正确反映了UH和UH2分子的结构特征 ,可用于研究UHH的微观反应动力学 .     

非谐环盘及变幅杆组成的变幅器动力学特性研究

为了解决齿轮超声加工振动系统的设计问题,将齿轮简化为环盘,根据环盘及变幅杆的力耦合条件、边界条件及动力学方程,推导了非谐环盘及变幅杆组成的变幅器的频率方程,并利用其设计了变幅器,实验证明:变幅器的谐振频率与理论设计结果基本一致。该理论方法使变幅器由全谐振设计拓展到非谐振设计。     

湿法消解-氢化物发生-原子荧光光谱法测定土壤中的铅

应用AFS-230E型双道原子荧光光谱仪进行了氢化物发生-原子荧光法测定土壤中铅的研究.方法中采用硝酸做介质,将铁氰化钾直接加入硼氢化钠-氢氧化钠碱性溶液中,并对各种最佳分析条件进行了探讨和验证.铅的校准曲线在0-100μg/L浓度范围内呈线性,方法的检出限为0.14μg/L,相对标准偏差为2.1％,回收率为91.0％-95.0％.用标准物质进行对照,其测定值在给定的标准值范围之内.该方法操作简单、灵敏度高、高效,适合土壤样品中铅的测定.