Immunological Detection of N‐formylkynurenine in Porphyrin‐Mediated Photooxided Lens α‐crystallin

Crystallin proteins are responsible for maintaining lens transparency and allowing the lens to focus light undistorted onto the retina. The α‐crystallins are the major lens crystallins, and function as both structural proteins and chaperones to protect all lens proteins from damage leading to lens deterioration. Because lens crystallin proteins do not turn over, the damage they accumulate can lead to cataracts, the world’s leading cause of blindness. Photosensitizing porphyrins can accumulate in the eye through either endogenous metabolism or through therapeutic or diagnostic procedures. Porphyrin buildup exacerbates lens aging through increased levels of singlet oxygen, resulting in protein polymerization and amino acid residue alteration. Tryptophans oxidize to kynurenine and N‐formylkynurenine (NFK) causing irreversible changes in the refractive index of the normally transparent lens, leading to development of cataracts. Additionally, NFK is itself a photosensitizer, and its presence exacerbates lens deterioration. This work uses anti‐NFK antiserum to study porphyrin‐facilitated photooxidation of α‐crystallin tryptophan residues. In vitro experiments show that four biologically interesting porphyrins mediate α‐crystallin polymerization and accumulation of both protein radicals and NFK. Confocal microscopy of cultured human lens epithelial cells indicates that while all four porphyrins photosensitize cellular proteins, not all oxidize the tryptophans of cellular α‐crystallin to NFK.     

Optimal Scaling of Random Walk Metropolis Algorithms with Non-Gaussian Proposals

The asymptotic optimal scaling of random walk Metropolis (RWM) algorithms with Gaussian proposal distributions is well understood for certain specific classes of target distributions. These asymptotic results easily extend to any light tailed proposal distribution with finite fourth moment. However, heavy tailed proposal distributions such as the Cauchy distribution are known to have a number of desirable properties, and in many situations are preferable to light tailed proposal distributions. Therefore we consider the question of scaling for Cauchy distributed proposals for a wide range of independent and identically distributed (iid) product densities. The results are somewhat surprising as to when and when not Cauchy distributed proposals are preferable to Gaussian proposal distributions. This provides motivation for finding proposal distributions which improve on both Gaussian and Cauchy proposals, both for finite dimensional target distributions and asymptotically as the dimension of the target density, d → ∞. Throughout we seek the scaling of the proposal distribution which maximizes the expected squared jumping distance (ESJD).     

Low loss broadband transmission in hypocycloid-core Kagome hollow-core photonic crystal fiber

We report on the fabrication of a seven-cell-core and three-ring-cladding large-pitch Kagome-lattice hollow-core photonic crystal fiber (HC-PCF) with a hypocycloid-shaped core structure. We demonstrate experimentally and theoretically that the design of this core shape enhances the coupling inhibition between the core and cladding modes and offers optical attenuation with a baseline of ～180?dB/km over a transmission bandwidth larger than 200?THz. This loss figure rivals the state-of-the-art photonic bandgap HC-PCF while offering an approximately three times larger bandwidth and larger mode areas. Also, it beats the conventional circular-core-shaped Kagome HC-PCF in terms of the loss. The development of this novel (to our knowledge) HC-PCF has potential for a number of applications in which the combination of a large optical bandwidth and a low loss is a prerequisite.     

Competing liquid phase instabilities during pulsed laser induced self-assembly of copper rings into ordered nanoparticle arrays on SiO2

Nanoscale copper rings of different radii, thicknesses, and widths were synthesized on silicon dioxide thin films and were subsequently liquefied via a nanosecond pulse laser treatment. During the nanoscale liquid lifetimes, the rings experience competing retraction dynamics and thin film and/or Rayleigh-Plateau types of instabilities, which lead to arrays of ordered nanodroplets. Surprisingly, the results are significantly different from those of similar experiments carried out on a Si surface. We use hydrodynamic simulations to elucidate how the different liquid/solid interactions control the different instability mechanisms in the present problem.     

Subsurface damage in alumina and alumina–silicon carbide nanocomposites

The subsurface plastic deformation below alumina (Al₂O₃) and Al₂O₃–silicon carbide (SiC) nanocomposite surfaces subjected to grinding, polishing and annealing has been measured by high-resolution grazing-incidence parallel-beam X-ray powder diffraction and transmission electron microscopy. The variation with angle in the full width at half-height maximum (FWHM) of the X-ray Bragg peaks was successfully modelled by a FWHM distribution that fell exponentially with increasing depth. Consistent parameters were extracted from data taken using both prism and pyramidal reflecting planes. Correlation was found between the depth at which the FWHM fell to 1/e of the surface value and the depth of damage observed by transmission electron microscopy. The associated surface strain in the nanocomposite was found to increase linearly with increasing diameter of the diamond polishing particles. In ground 5?vol.%?SiC nanocomposite, these random surface strains fell by a factor of 7 and the depth of damage increased by a factor of 3 after annealing at 1250°C for 2?h. No differences were observed in the Bragg peak FWHM as a function of angle for material polished with 1?µm diamond grit before and after annealing.     

Strain-rate dependence of the brittle-to-ductile transition temperature in tungsten

We have investigated the strain-rate dependence of the brittle-to-ductile transition (BDT) temperature in pre-cracked tungsten single-crystals and polycrystals. There is an unambiguous Arrhenius relationship over four decades of strain rate, giving an activation energy for the process controlling the BDT of 1.05?eV. This is equal to the activation energy for double-kink formation on screw dislocations, suggesting that their motion controls the brittle–ductile transition.     

New Light on Infrared Problems: Sectors,Statistics, Symmetries and Spectrum

A new approach to the analysis of the physical state space of a theory is presented within the general setting of local quantum physics. It also covers theories with long range forces, such as quantum electrodynamics. Making use of the notion of charge class, an extension of the concept of superselection sector, infrared problems are avoided by restricting the states to observables localized in a light cone. The charge structure of a theory can be explored in a systematic manner. The present analysis focuses on simple charges, thus including the electric charge. It is shown that any such charge has a conjugate charge. There is a meaningful concept of statistics: the corresponding charge classes are either of Bose or of Fermi type. The family of simple charge classes is in one-to-one correspondence with the irreducible unitary representations of a compact Abelian group. Moreover, there is a meaningful definition of covariant charge classes. Any such class determines a continuous unitary representation of the Poincaré group or its covering group satisfying the relativistic spectrum condition. The resulting particle aspects are also briefly discussed.     

Embedding Partial k‐Star Designs

A k‐star is the complete bipartite graph . Let G and H be graphs, and let be a partial H‐decomposition of G. A partial H‐decomposition, , of another graph is called an embedding of provided that and G is a subgraph of . We find an embedding of a partial k‐star decomposition of into a k‐star decomposition of , where s is at most if k is odd, and if k is even.     

Distinguishing dose populations in sediment mixtures: a test of single-grain optical dating procedures using mixtures of laboratory-dosed quartz

Many natural deposits contain grains that have different burial histories, but reliable procedures to extract the component doses from mixed-dose samples have not been developed in optical dating. Here we present results for synthetic two- and three-component mixtures of data derived from laboratory-dosed sedimentary quartz using a single-aliquot regenerative-dose protocol and statistical models for finite mixtures. Composite sets of data were created from the doses measured for individual grains that had been bleached by sunlight and then given a beta dose of 5, 10 or 20 Gy. We found that the correct number of dose components, corresponding doses, and relative proportions of each component may be estimated if the within-component dispersion is small or is known. We also found, however, that recuperation of the OSL signal in sun-bleached quartz may produce artificially high dose estimates for some grains, an effect that should be taken into account when modelling natural mixtures.