Study of mechanical motions in the basal region of the chinchilla cochlea

Measurements from the 1-4-mm basal region of the chinchilla cochlea indicate the basilar membrane in the hook region (12-18 kHz) vibrates essentially as it does more apically, in the 5-9-kHz region. That is, a compressive nonlinearity in the region of the characteristic frequency, amplitude-dependent phase changes, and a gain relative to stapes motion that can attain nearly 10,000 at low levels. The displacement at threshold for auditory-nerve fibers in this region (20 dB SPL) was approximately 2 nm. Measurements were made at several locations in individual animals in the longitudinal and radial directions. The results indicate that there is little variability in the phase of motion radially and no indication of higher-order modes of vibration. The data from the longitudinal studies indicate that there is a shift in the location of the maximum with increasing stimulus levels toward the base. The cochlear amplifier extends over a 2-3-mm region around the location of the characteristic frequency.     

Basilar-membrane response to multicomponent stimuli in chinchilla

The response of chinchilla basilar membrane in the basal region of the cochlea to multicomponent (1, 3, 5, 6, or 7) stimuli was studied using a laser interferometer. Three-component stimuli were amplitude-modulated signals with modulation depths that varied from 25% to 200% and the modulation frequency varied from 100 to 2000 Hz while the carrier frequency was set to the characteristic frequency of the region under study (approximately 6.3 to 9 kHz). Results indicate that, for certain modulation frequencies and depths, there is enhancement of the response. Responses to five equal-amplitude sine wave stimuli indicated the occurrence of nonlinear phenomena such as spectral edge enhancement, present when the frequency spacing was less than 200 Hz, and mutual suppression. For five-component stimuli, the first, third, or fifth component was placed at the characteristic frequency and the component frequency separation was varied over a 2-kHz range. Responses to seven component stimuli were similar to those of five-component stimuli. Six-component stimuli were generated by leaving out the center component of the seven-component stimuli. In the latter case, the center component was restored in the basilar-membrane response as a result of distortion-product generation in the nonlinear cochlea.     

Electroanalytical study of sulphamerazine at a glassy-carbon electrode and its determination in pharmaceutical preparations by HPLC with amperometric detection

An electroanalytical study of the oxidation of sulphamerazine at a glassy-carbon electrode has been made by use of different voltammetric techniques. The limiting current is diffusion-controlled, at pH 7.0, for sulphamerazine in the concentration range 2.0 x 10(-5)-1.0 x 10(-4)M. The limits of determination found for differential pulse voltammetry at a stationary electrode and at a rotating electrode were 5.9 x 10(-6) and 3.3 x 10(-6)M respectively. A reversed-phase HPLC method with amperometric detection for the analysis of sulphamerazine, sulphadiazine and phthalylsulphathiazole mixtures has been developed, and applied to a commercial pharmaceutical preparation.