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21.
The phosphoramidate-like reaction of 1,3-dithiole derived N-(diethoxyphosphinyl)hydrazones with formyl derivatives of 1,3-dithiole affords the longest aza-analogues of extended tetrathiafulvalenes with a polyenic spacer reported to date. Their structural and electrochemical properties are discussed. 相似文献
22.
Lead is extracted from urine with ammonium pyrrolidien dithiocrbamate into methyl isobutyl ketone, and 20 μl of the extract is injected into a water carrier stream in a flow-injection/atomic absorption system. The procedure is simple, quick, accurate and reproducible. Recoveries are 96–105%. The mean urine lead contents of 20 unexposed and 20 printing-press lead exposed workers was found to be 39.0 ± 8.3 and 71.7 ± 26.3 μg Pb l?1, respectively. 相似文献
23.
Raquel F. P. Nogueira Ronaldo A. Pilli Nelson durÁn 《Applied biochemistry and biotechnology》1992,33(3):169-176
Growth of the ascomyceteChrysonilia sitophila during degradation of lignin model dimers and monomers was compared to a glucose control. An inhibition of growth by Cα-carbonyl
monomers and stimulation by β-O-4 lignin model and vanillyl alcohol were observed. A comparison of the degradation by this
ascomycete with the basidiomycetePhanerochaete chrysosoporium showed similarities in relation to the type of degradation caused. 相似文献
24.
A.R. Sousa 《Polymer Degradation and Stability》2006,91(7):1504-1512
This work aims to analyze the effects of photodegradation on the stress cracking resistance of polystyrene. Injection moulded samples were exposed to the ultraviolet light for various times in the laboratory prior to solvent contact. The bars were then stressed in a tensile testing machine under the presence of butanol. During this period the stress relaxation was monitored and the ultimate properties were evaluated after selected periods of stress cracking. Complementary tests were done by size exclusion chromatography and by scanning electron microscopy. The results indicated that butanol causes significant modification in polystyrene, with extensive surface crazing as well as reduction in mechanical properties. This is intensified under higher mechanical stress. The previous degraded samples showed a higher level of stress relaxation and a greater loss in tensile strength in comparison to the undegraded ones. The synergist action of photodegradation and stress cracking in polystyrene may be a consequence of the chemical changes caused by oxidation like the formation of polar chemical groups and the reduction in molecular weight. 相似文献
25.
Pinotti Laura M. Silva Rosineide G. Giordano Roberto C. Giordano Raquel L. C. 《Applied biochemistry and biotechnology》2002,98(1-9):679-686
This article reports studies concerning the production of penicillin G acylase (PGA) by Bacillus megaterium. This enzyme has industrial use in the hydrolysis of penicillin G to obtain 6-aminopenicillanic acid, an essential intermediate
for the production of semisynthetic β-lactam antibiotics. Although most microorganisms produce the enzyme intracellularly,
B. megaterium provides extracellular PGA. The enzyme production by microorganisms involves several steps, resulting in a many operational
variables to be studied. The study of the inoculum is an important step to be accomplished, before addressing other issues
such as culture optimization and downstream processing. In this study, using a standard inoculum as reference, several runs
were performed aiming at the definition of operational conditions in the PGA production. Cell concentration and PGA activity
in the production medium were measured after 24, 48, and 72 h of the beginning of the production phase. This study encompasses
the duration of the inoculum germination phase and the concentration of cells used to startup the germination. Based on these
results, PGA productivity during the production phase was maximized. The selected values for these variables were 1.5 × 107 spores/mL of germination medium, germination during 24 h, and 72 h for the production phase. 相似文献
26.
The purpose of this study is to investigate the influence of nickel, which is an alloying element in commonly used metallic biomaterials, on the biomaterials mineralization process. An electrochemical method was developed to quantify this metal ion in osteoblast-like cell culture medium (OST) by performing adsorptive cathodic stripping voltammetry (CSV) with dimethylglyoxime (DMG) at a mercury film microelectrode (MFM). The optimized analytical conditions and the square-wave CSV parameters for the analysis are: DMG concentration: 5.00 × 10−4 mol L−1; ammonium chloride buffer: 0.10 mol L−1 (pH 9.2); frequency: 50 Hz, amplitude 20 mV; step: 2 mV; adsorption time: 10 s, deposition potential: −0.70 V and reduction potential: −1.20 V. The limit of detection was 7.70 × 10−9 mol L−1 for an adsorption time of 10 s. The results achieved by CSV using the MFM were compared to those obtained by atomic absorption spectrometry (AAS) to ensure the reliability of the electrochemical method. The mineralization process was evaluated by biochemical and histochemical assays. 相似文献
27.
Optimization of culture medium and conditions for penicillin acylase production by Streptomyces lavendulae ATCC 13664 总被引:1,自引:0,他引:1
Torres-Bacete J Arroyo M Torres-Guzmán R De La Mata I Acebal C Castillón MP 《Applied biochemistry and biotechnology》2005,126(2):119-132
The culture medium for Streptomyces lavendulae ATCC 13664 was optimized on a shake-flask scale by using a statistical factorial design for enhanced production of penicillin acylase. This extracellular enzyme recently has been reported to be a penicillin K acylase, presenting also high hydrolytic activity against penicillin V and other natural aliphatic penicillins such as penicillin K, penicillin F, and penicillin dihydroF. The factorial design indicated that the main factors that positively affect penicillin acylase production by S. lavendulae were the concentration of yeast extract and the presence of oligoelements in the fermentation medium, whereas the presence of olive oil in the medium had no effect on enzyme production. An initial concentration of 2.5% (w/v) yeast extract and 3 microg/mL of CuSO4 x 5H2O was found to be best for acylase production. In such optimized culture medium, fermentation of the microorganism yielded 289 IU/L of enzyme in 72 h when employing a volume medium/volume flask ratio of 0.4 and a 300-rpm shaking speed. The presence of copper, alone and in combination with other metals, stimulated biomass as well as penicillin acylase production. The time course of penicillin acylase production was also studied in the optimized medium and conditions. Enzyme production showed catabolite repression by different carbon sources such as glucose, lactose, citrate, glycerol, and glycine. 相似文献
28.
This paper reports the development of a method based on capillary electrophoresis with laser-induced fluorescence detection for the simultaneous determination of thiouracil (TU) and phenylthiouracil (PhTU) with high sensitivity (nanomolar range, i.e., attomoles detected). After derivatization with 5-iodoacetamidofluorescein, the analytes were separated by capillary zone electrophoresis using 20 mM phosphate buffer (pH 10.0) and quantified by fluorescence detection. The linearity range, precision, recovery, and detection limits were determined, and the method was shown to be applicable for the determination of TU and PhTU in spiked feed samples and urine. 相似文献
29.
Ribeiro da Silva MD Gomes JR Gonçalves JM Sousa EA Pandey S Acree WE 《The Journal of organic chemistry》2004,69(8):2785-2792
The mean (N-O) bond dissociation enthalpies were derived for three 2-methyl-3-(R)-quinoxaline 1,4-dioxide (1) derivatives, with R = methyl (1a), ethoxycarbonyl (1b), and benzyl (1c). The standard molar enthalpies of formation in the gaseous state at T = 298.15 K for the three 1 derivatives were determined from the enthalpies of combustion of the crystalline solids and their enthalpies of sublimation. In parallel, accurate density functional theory-based calculations were carried out in order to estimate the gas-phase enthalpies of formation for the corresponding quinoxaline derivatives. Also, theoretical calculations were used to obtain the first and second N-O dissociation enthalpies. These dissociation enthalpies are in excellent agreement with the experimental results herewith reported. 相似文献
30.
Arthur de Sousa 《Microchemical Journal》1973,18(2):137-141
An indirect microdetermination of phosphate via EDTA titration is described, which can be applied to minerals, soils, fertilizers, biological samples, drugs and organo-phosphorus compounds. The method is based on the precipitation of phosphate as the very insoluble silver orthophosphate (Ksp = 1.3 × 10?20), dissolution of this precipitate in a solution of potassium cyanonickelate and titration of the nickel displaced by silver. The phosphate content is obtained indirectly by multiplying the number of ml of the titrant by a factor. The method takes about an hour after the phosphate is brought into solution. The accuracy is about 1% for samples containing 5–50 mg PO43? and about 3–5% for samples with 100 to 5000 μg PO43?. 相似文献