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11.
Yoshihide Tanaka Tetsuji Higashi Randeep Rakwal Junko Shibato Shin-ichi Wakida 《高压研究》2013,33(4):519-523
Hydrostatic pressure is a physical factor that can induce stress in organisms. This stress leads to growth inhibition, cellular arrest, and cellular death, and these effects depend on the degree of pressure, temperature, and sensitivity of the organisms to hydrostatic pressure. Genomics studies of yeast cells under conditions recovering from high pressure-induced cellular damage showed evidence that multiprotein complexes or membrane proteins, and not soluble proteins, are the critical targets. We performed a metabolomic analysis. The metabolomics results suggested that membrane-spanning proteins broke down after high pressure treatment and recovery conditions. We also found 13 genes that were common to essential and pressure-induced gene groups. Among these 13 genes, more than 10 were associated with proteasome structure and functions. This suggests that proteasome structure or functions can be the critical target or a highly important factor. This hypothesis is supported by the fact that yeast cells are sensitive to the proteasome inhibitor MG132 after high pressure treatment. 相似文献
12.
Metabolomics is an emerging field in analytical biochemistry, and the development of such a method for comprehensive and quantitative analysis of organic acids, carbohydrates, and nucleotides is a necessity in the era of functional genomics. When a concentrated yeast extract was analyzed by CE-MS using a successive multiple ionic-polymer layer (SMIL)-coated capillary, the adsorption of the contaminants on the capillary wall caused severe problems such as no elution, band-broadening, and asymmetric peaks. Therefore, an analytical method for the analysis of anionic metabolites in yeast was developed by pressure-assisted CE using an inert polymer capillary made from poly(ether etherketone) (PEEK) and PTFE. We preferred to use the PEEK over the PTFE capillary in CE-MS due to the easy-to-use PEEK capillary and its high durability. The separation of anionic metabolites was successfully achieved with ammonium hydrogencarbonate/formate buffer (pH 6.0) as the electrolyte solution. The use of 2-propanol washing after every electrophoresis run not only eliminated wall-adsorption phenomena, but allowed for good repeatability to be obtained for migration times in the metabolomic analysis. 相似文献
13.
Tanvi?Dhiman Charanjeet?SinghEmail author Maalti?Puri Harpreet?Kaur Randeep?Kaur S.?Bindra Narang 《The European Physical Journal B - Condensed Matter and Complex Systems》2015,88(10):271
The microwave characteristics of Pb1?x Ca x Fe0.5Nb0.5O3 multiferroics (x = 0.0, 0.4, 0.45, 0.5, 0.55, 0.6), have been investigated as a function of frequency and substitution. The results depict ?13.99 dB reflection loss at 11.65 GHz in composition x = 0.6. Microwave absorption is enhanced with substitution of Ca2+ ions and undoped composition 0.0 behaves as electromagnetic shield. The model governing microwave absorption is discussed and different compositions for electromagnetic applications have been suggested. 相似文献
14.
Proteins extracted from green and etiolated shoots of rice were separated by two-dimensional polyacrylamide gel electrophoresis and relative molecular weights and isoelectric points were determined. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane and 85 proteins were analyzed by a gas-phase protein sequencer. The N-terminal amino acid sequences of 21 out of 85 proteins were determined in this manner. N-terminal regions of the remaining proteins could not be sequenced. The internal amino acid sequences of proteins were determined by sequence analysis of peptides obtained by the Cleveland peptide mapping method and compared with those of known plant and animal protein sequences to understand the nature of the proteins. Green shoots revealed the presence of photosynthetic proteins as expected; however, as etiolated shoots were not photosynthetic, only precursors of the photosynthetic proteins were identified. Interestingly, the presence of L-ascorbate peroxidase only in etiolated shoots suggests a cellular protectant function for this antioxidant enzyme in the etiolating shoots. Using this experimental approach, we could identify the major proteins involved in growth regulation in photosynthetic green shoots as well as in etiolating rice seedlings. 相似文献
15.
Theoretical modelling of miniature loop heat pipe 总被引:1,自引:0,他引:1
Randeep Singh Aliakbar Akbarzadeh Chris Dixon Masataka Mochizuki 《Heat and Mass Transfer》2009,46(2):209-224
Development in the design and thermal performance of the loop heat pipes (LHPs) demands the corresponding improvement in the
theoretical modeling capabilities of these devices. In this paper, mathematical model for assessing the thermal performance
of the miniature LHPs (mLHPs) on the basis of the operating temperature and thermal resistance of the loop has been discussed
in detail. In order to validate the theoretical model, a mLHP with the flat disk shaped evaporator, 30 mm in diameter and
10 mm thick, was developed and tested with nickel and copper wick structure. By comparison with experimental results, it was
found that the theoretical model was able to predict the evaporator temperature and loop thermal resistance very well and
within the uncertainties imposed by the underlying assumptions. The mathematical model can be used to validate the design
of the mLHP and verify whether the proposed design is consistent with the maximum heat load capacity required for the intended
application. In addition to this, the model can assists in understanding and refining the outcomes of the experimental studies. 相似文献