Modification of [VO(OPri)3] with oximes in different molar ratios, yielded new class of vanadia precursors, [VO{OPri}3?n{L}n] {where, n = 1–3 and LH = C9H16C=NOH (1–3) and (CH3)2C=NOH (4–6)}.All the products are yellow in colour. (1) and (2) are liquid/viscous liquid, while others are solids. Molecular weight measurements of all these derivatives and the ESI-mass spectral studies of (1), (2), (3) and (5) indicate their monomeric nature. 1H and 13C{1H} NMR spectra suggest that the oximato moieties are monodentate in solution which was further confirmed by the 51V NMR signals, appeared in the region expected for tetra-coordinated oxo-vanadium atoms. On ageing, a disproportionation reaction occurs in (1) and some crystals appeared. Single crystal X-ray diffraction analyses of the crystals obtained from (1) as well as from (3) were found to be the same and indicate the presence of side-on {dihapto η2-(N, O)} binding modes of the oximato ligands, leading to the formation of seven coordination environment around the vanadium atom. Thermogravimetric curve of (1) exhibits multi-step decomposition with the formation of V2O5 as the final product at ~850 °C. Sol–gel transformation of (3) yielded (a) VO2 sintered at 300 °C and (b) V2O5 at 600 °C. Similarly, sol–gel transformations of (1) and (2) yielded V2O5 (c) and (d) at 600 °C, respectively. Formation of monoclinic phase in (a) and orthorhombic phase in (b), (c) and (d) were confirmed by powder XRD patterns. 相似文献
Some oxime modified complexes of the type [Zr{OPri}4?n{L}n] {where, n = 1–4 and LH=(CH3)2C=NOH (1–4) and C9H16C=NOH (5–8)} have been synthesized by the reaction of [Zr(OPri)4·PriOH] with oximes, in anhydrous refluxing benzene. These synthesized complexes were characterized by elemental analyses, molecular weight measurements, ESI-mass, FT-IR and NMR (1H and 13C{1H}) spectral studies. The ESI-mass spectral studies indicate dimeric nature for [Zr{OPri}2{ONC(CH3)2}2] (2), [Zr{OPri}3{ONC10H16}] (5) and [Zr{OPri}{ONC10H16}3] (7) and monomeric nature for [Zr{ONC10H16}4] (8). Oximato ligands appear to bind the zirconium in side on manner in all the complexes. Thermogravimetric curves of (2) and (8) exhibit multi-step decomposition with the formation of ZrO2, under nitrogen atmosphere. Sol–gel transformations of precursors (5), (6), (7) and (8) in organic medium, yielded nano-sized tetragonal phase of zirconia samples (a), (b), (c) and (d), respectively, on sintering at ~600 °C. All these samples were characterized by Powder XRD patterns and EDX analyses. Surface morphologies of these samples were investigated by SEM images. 相似文献
The highly conserved HIV-1 transactivation response element (TAR) binds to the trans-activator protein Tat and facilitates viral replication in its latent state. The inhibition of Tat–TAR interactions by selectively targeting TAR RNA has been used as a strategy to develop potent antiviral agents. Therefore, HIV-1 TAR RNA represents a paradigmatic system for therapeutic intervention. Herein, we have employed biotin-tagged TAR RNA to assemble its own ligands from a pool of reactive azide and alkyne building blocks. To identify the binding sites and selectivity of the ligands, the in situ cycloaddition has been further performed using control nucleotide (TAR DNA and TAR RNA without bulge) templates. The hit triazole-linked thiazole peptidomimetic products have been isolated from the biotin-tagged target templates using streptavidin beads. The major triazole lead generated by the TAR RNA presumably binds in the bulge region, shows specificity for TAR RNA over TAR DNA, and inhibits Tat–TAR interactions. 相似文献
A bio-inspired method is used to render controlled wrinkling surface patterns on supramolecular architectures assembled from polyoxometalate (POM) clusters. It involves a polyamine-multivalent anion interaction generating positively charged coacervates, which while dictating the assembly of POM into spherical structures further facilitate an interesting surface morphogenesis with wrinkling patterns. This spontaneous surface wrinkling depends on the type of multivalent anion and the pH. As the polyamine-anion interaction becomes stronger, the wrinkles turn denser with lesser depth, which eventually undergoes post-buckling to engender a complex surface pattern. Interestingly, the order of influence exerted by different anions on the morphology follows the Hofmeister series. Moreover, the mild synthesis conditions keep the functional POM units dispersed in the sphere with a structural transformability to their lacunary form. 相似文献
A series of N-dibenzosuberene substituted aroyl selenourea ligands L1–L3 and their Ru(II) (η6-p-cymene) complexes 1–3, [Ru(II) (η6-p-cymene) L] (L?=?monodentate aroyl selenourea ligand) were synthesized and characterized. The molecular structures of the ligand L3 and complex 3 were confirmed by single-crystal XRD method. The single-crystal XRD study results revealed that aroyl selenourea ligand coordinates with ruthenium via Se neutral monodentate atom. In vitro DNA interaction studies were investigated by UV–Visible and fluorescence spectroscopic methods which showed that the intercalative mode of binding is in the order of 3?>?2?>?1 with the Ru(II) (η6-p-cymene) complexes. The binding affinity of the bovine serum albumin with complexes was calculated using spectroscopic methods. Quantum chemical computations were made using DFT (density functional theory), BL3YP; LANL2DZ basis set in order to determine the frontier molecular orbital parameters and MESP for the newly synthesized complexes. The complexes 1–3 have shown intensive cytotoxicity against the cancer lines HepG-2 and A549 under in vitro conditions. Complex 3 (IC50?=?62 μM) has shown significant cytotoxic activity against HepG-2 compared to cisplatin standard drug. The complexes also examined for their antimicrobial activity. The complex 2 exhibited good activity against B. subtilis (MIC: 13.60 μg/mL), E. coli (MIC: 8.01 μg/mL) and A. flavus (MIC?=?15.60 μg/mL), respectively, compared to reference drugs Streptomycin and Ketoconazole.
Burn wound healing remains a challenging health problem worldwide due to the lack of efficient and precise therapy. Inherent oxidative stress following burn injury is importantly responsible for prolonged inflammation, fibrotic scar, and multiple organ failure. Herein, a bioinspired antioxidative defense system coupling with in situ forming hydrogel, namely, multiresponsive injectable catechol‐Fe3+ coordination hydrogel (MICH) matrix, is engineered to promote burn‐wound dermal repair by inhibiting tissue oxidative stress. This MICH matrix serves as the special traits of “Fe‐superoxide dismutases,” small molecular antioxidant (vitamin E), and extracellular matrix (ECM) in alleviating cellular oxidative damage, which demonstrates precise scavenging on reactive oxygen species (ROS) of different cellular locations, blocking lipid peroxidation and cell apoptosis. In in vivo burn‐wound treatment, this MICH promptly integrates with injured surrounding tissue to provide hydration microenvironment and physicochemical ECM for burn wounds. Importantly, the MICH matrix suppresses tissue ROS production, reducing the inflammatory response, prompting re‐epithelization and neoangiogenesis during wound healing. Meanwhile, the remodeling skin treated with MICH matrix demonstrates low collagen deposition and normal dermal collagen architecture. Overall, the MICH prevents burn wound progression and enhances skin regeneration, which might be a promising biomaterial for burn‐wound care and other disease therapy induced by oxidative stress. 相似文献
This paper identifies laminar separation bubbles at the root or span-wise midsection of a rectangular wing using direct surface pressure measurements in the wind tunnel and analyses their behavior at different Reynolds numbers and angles of attack. The separation, transition, and reattachment locations are determined as functions of the angles of attack and the Reynolds number. The transition structure and turbulence characteristics in the separated shear layer are studied using laser Doppler velocimetry. Surface pressure data and simultaneously acquired velocity signals are correlated to show the pattern of growing disturbances in the shear layer. Surface oil flow visualizations clarified the wingtip and separation bubble’s interactions near the leading edge of the wing at the higher angles of attack. Turbulence statistics are also calculated from the streamwise velocity distributions, and an apparent deviation is observed for the skewness and flatness values from the normal distributions in the near-wall region. The separation bubble effect on aerodynamic coefficients of a 3D rectangular wing root section is studied and reported.
Lanreotide, a somatostatin analogue, was radioiodinated with 125I to explore the possibility of using 123I labeled lanreotide as a diagnostic radiopharmaceutical for tumors overexpressing somatostatin (SST) receptors. Radioiodination
was carried out with 125I using chloramine T as the oxidant. The labeling yield was >90%. Characterization of 125I-Lanreotide was carried out by paper electrophoresis as well as HPLC. 125I-Lanreotide was purified by chromatography using a C18 Sep-Pak column. Radiochemical purity of the purified 125I-Lanreotide thus obtained was >99%. Significant tumor uptake of 125I-Lanreotide was observed in C57BL/6 mice bearing melanoma. 相似文献