Enantiomerically enriched trans-diols from alkenes in one pot: a multicatalyst approach

Multicatalysts consisting of non-natural oligopeptides with distinctly different catalytic moieties create molecular complexity in a multistep one-pot sequence starting from simple alkenes yielding highly enantiomerically enriched trans-diols.     

A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element

The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA–protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug‐metabolizing enzymes and many other genes. Radiolabeled ³²P‐DNA‐probes had been used in studies of PXR‐DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences.     

Enantioseparation of tartaric acid by ligand‐exchange capillary electrophoresis using contactless conductivity detection

A method for the determination of tartaric acid enantiomers using CE with contactless conductivity detection has been developed. Cu(II) as a central metal ion together with l ‐hydroxyproline were used as a chiral selector, the BGE was composed of 7 mM CuCl_2, 14 mM trans‐4‐hydroxy‐l ‐proline, and 100 mM ε‐aminocaproic acid; the pH was adjusted to 5 by hydrochloric acid. Separation with a resolution of 1.9 was achieved in 9 min in a polyacrylamide‐coated capillary to suppress the EOF. Various counterions of the BGE were studied, and migration order reversal was achieved when switching from ε‐aminocaproic acid to l ‐histidine. With detection limits of about 20 μM, the method was applied to the analysis of wine and grape samples; only l ‐tartaric acid was found.     

C6‐substituted purine derivatives: an experimental and theoretical 1H, 13C and 15N NMR study

We measured the ¹H, ¹³ C and ¹⁵ N chemical shifts for a series of purine derivatives bearing a norbornane substituent in position 9 and various substituents in position 6. The experimental data were complemented with density functional theory (DFT) calculations. The comparison of the calculated and experimental chemical shifts provided us with information about the tautomer and conformational equilibria of the studied compounds. Copyright © 2012 John Wiley & Sons, Ltd.     

RP-ZrO2 Stationary Phase as an Alternative to Separate of Doxazosin Impurities

Degradation products and related compounds occurring in drugs as impurities have often very similar properties as the parent substance. Therefore new analytical separation methods which show appropriate selectivity are required. Apart from improvements on silica-based reversed phases the attention has also been focused on other materials such as modified inorganic supports. In this study a polystyrene-coated zirconia was examined as an alternative in analysis of parent drug (doxazosin) and its impurities. Doxazosin and its five pharmacopoeial impurities were separated within 16 min. The suitability of the developed method was verified and confirmed by means of linearity, precision, inter-day precision, robustness and determination of LOD as well as LOQ. The results proved the potential of zirconia-based stationary phases to be useful alternatives in the field of drug analysis.     

Concepts and fuzzy sets: Misunderstandings, misconceptions, and oversights

The psychology of concepts has been undergoing significant changes since the early 1970s, when the classical view of concepts was seriously challenged by convincing experimental evidence that conceptual categories never have sharp boundaries. Some researchers recognized already in the early 1970s that fuzzy set theory and fuzzy logic were potentially suitable for modeling of concepts and obtained encouraging results. This positive attitude abruptly changed in the early 1980s, and since that time fuzzy set theory and fuzzy logic have been portrayed as problematic and unsuitable for representing and dealing with concepts. Our aim in this paper is to identify some of the most notorious claims regarding fuzzy set theory and fuzzy logic that have propagated through the literature on psychology of concepts and to show that they are, by and large, false. We trace the origin and propagation of these claims within the literature in this area. It is shown in detail that these claims are consistently erroneous and that they are based on various misunderstandings, misconceptions, and oversights. The ultimate purpose of this paper is to document these various erroneous claims.     

Experimental demonstration of coherent state estimation with minimal disturbance

We investigate the optimal trade-off between information gained about an unknown coherent state and the state disturbance caused by the measurement process. We propose several optical schemes that can enable this task, and we implement one of them, a scheme that relies on only linear optics and homodyne detection. Experimentally we reach near optimal performance, limited only by detection inefficiencies. In addition, we show that such a scheme can be used to enhance the transmission fidelity of a class of noisy channels.     

Birkhoff variety theorem and fuzzy logic

An algebra with fuzzy equality is a set with operations on it that is equipped with similarity , i.e. a fuzzy equivalence relation, such that each operation f is compatible with . Described verbally, compatibility says that each f yields similar results if applied to pairwise similar arguments. On the one hand, algebras with fuzzy equalities are structures for the equational fragment of fuzzy logic. On the other hand, they are the formal counterpart to the intuitive idea of having functions that are not allowed to map similar objects to dissimilar ones. In this paper, we present a generalization of the well-known Birkhoffs variety theorem: a class of algebras with fuzzy equality is the class of all models of a fuzzy set of identities iff it is closed under suitably defined morphisms, substructures, and direct products. and Institute for Fuzzy Modeling, University of Ostrava, 30. dubna 22, 701 03 Ostrava, Czech Republic Mathematics Subject Classification (2000):03B52, 08B05     

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer‐protein formation at the surface of CD‐trodes (GCDTs) in the presence of the redox couple [Fe(CN)₆]^3?/4?. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self‐assembling of a thiol‐modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 µM with a limit of detection of 5 nM.