排序方式: 共有52条查询结果,搜索用时 31 毫秒
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水溶性共轭聚合物研究属于国际前沿研究领域,因其具有水溶性和超级信号放大作用,在生物传感领域展现出良好的应用前景.但由于传统水溶性线型共轭高分子两亲性结构特点,其含有的憎水性刚性共轭链在水溶液中易聚集,水分子对共轭链激发态的影响导致其发光效率低(一般小于30%),严重制约了其应用与拓展.如何方便有效构筑高分子的高支化是获得高荧光效率的关键.在本篇综述中,我们综述了水溶性共轭聚合物分子刷的合成技术,分析了其结构与光学性质的关系,为水溶性共轭聚合物分子刷的设计和制备提供指导.另外,总结了水溶性共轭聚合物分子刷在化学生物传感、生物成像、药物运输、癌症治疗等领域的应用,最后对水溶性共轭聚合物分子刷存在的主要问题以及未来的热点方向进行了分析和展望! 相似文献
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以刷子状水溶性共轭聚芴(PFNI)为传感材料,以荧光素标记的核酸适体(FAM-apt15)为探针,设计了一种检测凝血酶的高灵敏度蛋白质传感器. PFNI的刷状结构带有大量正电荷,与负电荷的柔性单链核酸探针形成静电复合物,使能量供体(PFNI)与受体(FAM)之间的距离较近,发生高效荧光共振能量转移(Föster resonance energy transfer,FRET). 当探针与靶凝血酶结合时,形成刚性且体积较大的G-四链体/凝血酶复合物,由于体积位阻和密集的刷子的阻碍作用,PFNI与FAM之间的距离被拉大,FRET效率显著降低. 对缓冲溶液中凝血酶检测的最低检测限可达0.05 nmol/L. 与基于线型共轭聚合物的蛋白质检测方法相比,灵敏度提高了至少一个数量级. 相似文献
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Photo-induced excited-state dynamics within organic materials fundamentally determine their photophysical properties for various applications, and thus understanding the primary excited-state dynamics behavior is of fundamental and practical significance. Until recently, the excited-state dynamics of organic materials towards biophotonics have been rarely studied,although numerous endeavors have been devoted to the design of organic materials for biophotonics. Herein, various spectroscopy technologies including femtosecond transient absorption(fs-TA) spectroscopy clearly reveal a totally different excited state dynamics behavior within Bodipy monomer(2B-BDP dye) and aggregates(2B-BDP NPs), indicating strongly morphology dependent character. 2B-BDP dye undergoes an ultrafast conversion from S_1 to intramolecular charge transfer(ICT) state for subsequent photoluminescence(PL) and nonradiative(NR) decay, while 2B-BDP NPs show an accelerated excited-state deactivation mainly through S_1→S_0 NR decay. The potential bioapplications based on the corresponding excited state dynamics behavior are discussed together with experimental results. Interestingly, the accelerated NR decay in 2B-BDP NPs does not yield a stronger photoacoustic(PA) signal than that in 2B-BDP dye, which violates the conventional wisdom that faster NR decay would benefit the photothermal effects for better photoacoustic imaging(PAI). These insightful and fundamental observations of the excited-state dynamics may contribute an alternative approach at the molecular level towards the future design of functional Bodipy-based organic molecules with desirable performances. 相似文献
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共轭聚合物具有电子高度离域的共轭结构及优异的光吸收和发射特性,在有机电子、化学/生物传感、医学诊断及生物成像等领域具有广泛的应用。特定基团功能化的共轭聚合物含有可特异性识别生物/化学分子的功能基团(如糖基、生物素、羧基、氨基酸、肽段、核酸、抗体、氨基、巯基等),进一步拓宽了共轭聚合物在生物/化学分析领域的应用。本文从功能化基团的不同类别出发,对近年来共轭聚合物的功能化方法及其在生物/化学分析领域的应用(如蛋白质、病原体、Hg2+、Pb2+检测)进行了综述,并对该领域的发展前景进行了展望。 相似文献
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近年来,近红外二区(NIR-II,1000~1700 nm)荧光成像因其较高的空间分辨率、较深的组织穿透能力,在分子影像领域引起了广泛的关注。常见的NIR-II发光材料(如有机小分子、共轭聚合物、量子点等)通常具有光稳定性差、荧光量子产率低、斯托克斯位移小、荧光峰宽等问题,限制了这一新型成像技术的进一步发展与应用。稀土纳米材料由于其独特的发光特性,能够较好地克服这些不足,近年来不同结构的稀土纳米材料也逐渐被设计开发并应用于近红外二区荧光成像与检测,展示出了巨大的应用潜力。本综述首先介绍了稀土纳米材料的光学特性,然后按敏化离子的不同(Yb3+、Nd3+、Er3+、Tm3+)详细介绍了近红外二区稀土纳米材料的设计方法及相关应用,最后对稀土纳米材料在近红外二区成像领域的进一步发展进行了展望。 相似文献
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Yuqi Wang Xiaoming Hu Jianhui Weng Jinbo Li Quli Fan Yan Zhang Deju Ye 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(15):4940-4944
Photoacoustic (PA) imaging shows promise in the sensitive detection of caspase‐3 activated in early tumor apoptosis in response to chemotherapy; smart PA probes are thus in high demand. Herein, we report the first smart PA probe ( 1‐RGD ) responsive to caspase‐3, enabling real‐time and high‐resolution imaging of tumor apoptosis. 1‐RGD is designed to leverage the synergetic effect of active delivery and caspase‐3 activation. It is selectively recognized by active caspase‐3 to trigger peptide substrate cleavage and biocompatible macrocyclization‐mediated self‐assembly, leading to an amplified PA imaging signal and prolonged retention in apoptotic tumor cells. Strong, high‐resolution PA images are obtained in chemotherapy‐induced apoptotic tumors in living mice after intravenous administration with 1‐RGD , facilitating sensitive reporting of caspase‐3 activity and distribution within tumor tissues. 相似文献
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