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961.
A Small‐Molecule FRET Reporter for the Real‐Time Visualization of Cell‐Surface Proteolytic Enzyme Functions
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Jing Mu Dr. Fang Liu Muhammad Shafiq Rajab Meng Shi Shuang Li Chiching Goh Prof. Lei Lu Prof. Qing‐Hua Xu Prof. Bin Liu Dr. Lai Guan Ng Prof. Bengang Xing 《Angewandte Chemie (International ed. in English)》2014,53(52):14357-14362
Real‐time imaging of cell‐surface‐associated proteolytic enzymes is critical to better understand their performances in both physiological and pathological processes. However, most current approaches are limited by their complexity and poor membrane‐anchoring properties. Herein, we have designed and synthesized a unique small‐molecule fluorescent probe, which combines the principles of passive exogenous membrane insertion and Förster resonance energy transfer (FRET) to image cell‐surface‐localized furin‐like convertase activities. The membrane‐associated furin‐like enzymatic cleavage of the peptide probe leads to an increased fluorescence intensity which was mainly localized on the plasma membrane of the furin‐expressed cells. This small‐molecule fluorescent probe may serve as a unique and reliable reporter for real‐time visualization of endogenous cell‐surfaceassociated proteolytic furin‐like enzyme functions in live cells and tissues using one‐photon and two‐photon microscopy. 相似文献
962.
Gabor Butora Ning Qi Wenlang Fu Truyen Nguyen Hsueh‐Cheng Huang Ian W. Davies 《Angewandte Chemie (International ed. in English)》2014,53(51):14046-14050
The cytosolic conversion of therapeutically relevant nucleosides into bioactive triphosphates is often hampered by the inefficiency of the first kinase‐mediated step. Nucleoside monophosphate prodrugs can be used to bypass this limitation. Herein we describe a novel cyclic‐disulfide class of nucleoside monophosphate prodrugs with a cytosol‐specific, reductive release trigger. The key event, a charge‐dissipating reduction‐triggered cyclodeesterification leads to robust cytosolic production of the cyclic 3′,5′‐monophosphate for downstream enzymatic processing. The antiviral competence of the platform was demonstrated with an O‐benzyl‐1,2‐dithiane‐4,5‐diol ester of 2′‐C‐methyluridine‐3′,5′‐phosphate. Both in vitro and in vivo comparison with the clinically efficacious ProTide prodrug of 2′‐deoxy‐2′‐α‐fluoro‐β‐C‐methyluridine is provided. The cytosolic specificity of the release allows for a wide range of potential applications, from tissue‐targeted drug delivery to intracellular imaging. 相似文献
963.
Dr. Qing‐Fu Sun Dr. Sota Sato Prof. Dr. Makoto Fujita 《Angewandte Chemie (International ed. in English)》2014,53(49):13510-13513
In the self‐assembly of PdII ions and two different, but similarly shaped, ligands ( 1 and 2 ), neither random mixing nor self‐sorting of the two ligands into two unmixed structures was observed. Instead a mixed, yet sorted, Pd12( 1 )12( 2 )12 cantellated tetrahedron (and its pseudoisomer) was selectively formed, thus revealing a fine example of intramolecular self‐sorting. A case study showed that a homothetic ratio of >2 is necessary to observe cantellated tetrahedra. 相似文献
964.
Youqian Bai Mengyu Gan Wei Qiu Feifei Cheng Dandan Fu Zhitao Li Sha Li 《Polymer Science Series B》2014,56(3):377-383
The polyaniline-gold (PANI-AuNPs) fibrillar nanocomposites were prepared via a simple one-pot synthesis route in ethylene glycol (EG) medium. The morphology of PANI-AuNPs was characterized by transmission electron microscopy (TEM), and their electrochemical properties were studied by CV, TAF and EIS measurement using an electrochemical workstation. The results show that the morphology of nanocomposites can be controlled by changing the temperature. The diameter of PANI-AuNPs composite nanofiber reduce from about 80 to 30 nm, and the size of AuNP also reduce from about 20 to 4 nm, with the rise of synthesized temperature. When the synthesized temperature is 10°C, the distribution of AuNPs is the best, and the amount of AuNPs dispersed in PANI is the most, as well as the electrochemical performance of PANI-AuNPs is the most excellent. 相似文献
965.
Ultrasound/microwave‐assisted extraction and comparative analysis of bioactive/toxic indole alkaloids in different medicinal parts of Gelsemium elegans Benth by ultra‐high performance liquid chromatography with MS/MS
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Indole alkaloids are the main bioactive/toxic components in Gelsemium elegans Benth. To determine the distribution and contents of indole alkaloids in its different medicinal parts, a novel and rapid method using ultra‐high performance LC (UPLC) with MS/MS has been established and validated with an optimized ultrasound/microwave‐assisted extraction method. Four constituents, namely, humantenidine, humantenmine, gelsemine, and koumine, were simultaneously determined in 6 min. Chromatographic separation was achieved on an ultra‐high performance LC BEH C18 column with a gradient mobile phase consisting of methanol and water (containing 0.1% formic acid both in methanol and water) at a flow rate of 0.3 mL/min. The detection was performed on a triple quadrupole electrospray MS/MS by positive ion multiple‐reaction monitoring mode. All the analytes showed good linearity (r ≥ 0.9934) within a concentration range from 0.1–25 μg/mL with a LOQ of 25–50 ng/mL. The overall intra‐ and intervariations of four components were <4.7% with an accuracy of 97.3–101.3%. The analysis results showed that there were remarkable differences in the distribution and contents of four chemical markers in the roots, stems, and leaves of G. elegans Benth. The findings can provide necessary and meaningful information for the rational utilization of its resources. 相似文献
966.
固相萃取-气相色谱-串联质谱法测定乳胶儿童用品中15种N-亚硝胺及其前体物的迁移量 总被引:1,自引:0,他引:1
建立了同时测定乳胶儿童用品中15种N-亚硝胺及其前体物迁移量的固相萃取-气相色谱-串联质谱(SPEGC-MS/MS)分析方法。以人工唾液作为迁移模拟物,以Chromabond Easy固相萃取柱(填料的主要成分是极性修饰的聚乙烯-二乙烯基苯共聚物)对迁移液中的N-亚硝胺分析物进行净化,采用HP-5 MS UI色谱柱分离,MS/MS在多反应监测模式下进行定性及定量分析。15种N-亚硝胺在5~2 000μg/L范围内呈良好的线性关系,相关系数均大于0.998;方法定量限(S/N=10)为0.625~12.50μg/kg,低于欧盟2 009/48/EC指令的限量要求。在低、中、高3个添加水平的回收率为53.8%~116.2%、52.7%~105.1%和49.5%~102.9%;日内精密度分别为1.3%~14.0%(n=6),日间精密度为1.6%~7.6%(n=4)。采用本方法对婴儿奶嘴样品和气球样品进行了测定,其中4件奶嘴和7件气球样品中检出亚硝胺及其前体物,奶嘴和气球中N-亚硝胺的总检出含量分别为0.049 9~0.126mg/kg和0.515~41.2 mg/kg;N-亚硝胺前体物总检出量分别为0.026 4~0.030 0 mg/kg和0.187~12.5mg/kg。 相似文献
967.
以3,3′,5-联苯三羧酸(biphenyl-3,3′,5-tricarboxylic acid,H3bpta)、2,6-二(1-咪唑基)吡啶(2,6-bis(imidazole-1-yl)pyridine,bip)、Zn(NO3)2·6H2O和ZnCl2为原料,在水热条件下合成了配位聚合物{[Zn3(H2O)7(bpta)2]·5H2O}n(1)和{[Zn2Cl(bpta)(bip)2]·2H2O}n(2)。并利用红外、元素分析和X-射线单晶衍射等对其结构进行表征。X-射线单晶衍射分析表明:化合物1属于单斜晶系,C2/c空间群,a=3.317 1(11)nm,b=1.495 7(5)nm,c=0.695 1(2)nm,β=91.50°,Z=4;化合物2属于单斜晶系,P2/c空间群,a=1.960 4(4)nm,b=1.035 7(2)nm,c=1.998 7(4)nm,β=101.97(3)°,Z=4。化合物1通过bpta桥联Zn髤形成1D链,通过配位水与羧基氧之间的氢键作用构筑成3D结构。化合物2中bip桥联Zn髤构成1D螺旋链状结构,进一步通过bpta桥连形成2D网状结构。此外,对化合物1和2进行了热稳定性分析和荧光性质研究。 相似文献
968.
提出了高效液相色谱-串联质谱法同时测定动物源性食品中35种兽药残留量的方法。样品经含1%(体积分数)乙酸的乙腈提取,QuEChERS方法净化,所得净化液以Zobax Eclipse Plus C18色谱柱为分离柱,以不同体积比的0.1%甲酸(体积分数)溶液和乙腈混合液为流动相进行梯度洗脱,采用电喷雾正离子源和多反应监测模式检测。35种化合物的质量分数均在1~50μg·kg-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.02~1.07μg·kg-1之间,测定下限(10S/N)在0.08~3.58μg·kg-1之间。以空白样品为基体进行加标回收试验,所得回收率在45.6%~121%之间,相对标准偏差(n=6)在2.4%~24%之间。 相似文献
969.
970.
分别以硼氨配合物和硼氢化钠为还原剂合成了核壳结构的Cu@CoW三元合金催化剂和非核壳结构的CuCoW三元合金催化剂,25℃下,Cu0.4@Co0.5W0.1三元合金催化剂对于硼氨配合物水解反应的TOF(转换频率)值达到0.369 0 molH2·molcat-1·s-1,明显高于非核壳结构的Cu0.4Co0.5W0.1催化剂,接近Pt、Pd等贵金属的催化活性,反应的活化能为49 kJ·mol-1。与非核壳结构的CuCoW合金相比,核壳结构的Cu@CoW三元合金催化剂的催化性能及稳定性均有明显提高。 相似文献