Resonance-stabilized phenoxy-like radicals formed by NO-mediated mono-nitration of coumarins containing a hydroxy group

Stable aminoxyls and an iminoxyl were observed by spin trapping and EPR techniques during the nitration of coumarins containing a hydroxy group by nitric oxide. The trapped free radicals are deduced to be the resonance stabilized phenoxy-like radicals. The mechanisms for the nitrations are suggested.     

Ferritin immunosensing on microfabricated electrodes based on the integration of immunoprecipitation and electrochemical signaling reactions.

A signal registration strategy from micropatterned immunosensors that converts antigen-antibody binding reactions into electrochemical signals was demonstrated. An array-type micropatterned gold electrode on a silicon wafer was fabricated, containing two electrode geometries of rectangular (100 microm x 500 microm) and circular (r. 50 microm) types, exhibiting electrochemical characteristics of bulk and micro-electrodes, respectively. Ferritin was employed as a model analyte for immunosensing because it has an advantageous molecular structure for functionalization to the sensing interface, and is regarded as a general marker protein for tumors and cancer recurrence. With the fabricated and ferritin-functionalized immunosensors, biospecific interactions were performed with antiferritin antiserum and secondary antibody samples, followed by electrochemical signaling via an immunoprecipitation reaction by the label enzyme. Under the optimized affinity-surface construction steps and reaction conditions, both types of microfabricated electrodes exhibited well-defined calibration results as a function of the protein concentration in antiserum samples. Furthermore, circular-type micropatterned immunoelectrodes exhibited voltammetric characteristics of microelectrodes, which is advantageous in terms of sensor operation under a fixed potential and low signal drift during the signaling reaction compared with the bulk-type electrodes. The results support that the employed signaling method with the proposed immunosensor configuration is fit for sensor miniaturization and integration to future biomicrosystems.     

Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the P53 gene.

Fluorescent-based single-strand conformation polymorphism (F-SSCP) analysis with capillary electrophoresis (CE) is the most common method for the detection of mutation because of its high sensitivity and resolution. In this study, we prepared an inexpensive linear polyacrylamide (LPA), and successfully applied it to CE-SSCP analysis and tandem CE-SSCP/heteroduplex analysis (HA) of the P53 gene on an ABI capillary genetic analyzer. A comparison of the sieving capabilities of a homemade LPA and commercial polydimethylacrylamide (PDMA) demonstrates that the homemade LPA has a higher resolution, a shorter analysis time, and is more suitable for tandem SSCP/HA than commercial PDMA. To show the usefulness, mutations of P53 gene exon 7 - 8 in 37 tumor samples were investigated by using homemade LPA. The results indicate that 10 mutations were found in 9 of 37 cases; the majority of P53 mutations were missense mutations, and 70% were located in exon 7, which plays an important role in neoplastic progression in human tumorigenesis.     

BMAP/G/1 queue with correlated arrivals of customers and disasters

We consider a single server queue with disasters where the arrivals of customers and disasters are correlated. When a disaster occurs, it removes all the customers in the system and there requires repair time for the system to be operated normally. The stationary queue length distribution at the embedded points and at an arbitrary time are presented.     

Continuous dependence on modelling for a complex Ginzburg–Landau equation with complex coefficients

Continuous dependence on a modelling parameter is established for solutions of a problem for a complex Ginzburg–Landau equation. A homogenizing boundary condition is also used to discuss the continuous dependence results. We derive a priori estimates that indicate that solutions depend continuously on a parameter in the governing differential equation. Copyright © 2004 John Wiley & Sons, Ltd.     

Spectra Analysis of a Novel Ti-Doped LiAlO2 Single Crystal

LiAlO2 single crvstals doped with Ti at concentration 0.2at.% are grown by the Czochralskl technique with dimensions φ42×55mm. Ti ions in the crystal are quadrivalence proven by comparing the absorption and fluorescence spectra of pure LiAlO2 and Ti： LiAlO2. After air and Li-rich atmosphere annealing, the absorption peaks in the range of 600-800nm disappear. We conclude that 682 and 756nm absorption peaks are attributed to the VLi and Vo absorptions, respectively： The peaks at 716nm and 798nm may stem from the VLi^＋ and absorptions. The colour-centre model can be applied to explain the experimental phenomena. Ti^4＋-doping produces more lithium vacancies in the LiAlO2 crystal. The intensities of [LiO4] and the associated bonds remain unchanged, which improves the anti-hydrolyzation and thermal stability of LiAlO2 crystals.     

Boundedness of Multilinear Operators on Herz Type Spaces： Extreme Cases

In this paper, two classes of closely related multilinear singular and fractional integrals,which include the commutators as special cases, are studied and their boundedness on Herz type spaces is discussed. In fact, it is proved that these operators are actually not bounded in certain extreme cases.     

KDP晶体三倍频实验和分析

报道了ＫＤＰ晶体中的三倍频产生的实验研究，计算了比较了直接三倍频过程和级联过程对ＫＤＰ晶体三阶有效非线性系数的贡献，并探讨了ＫＤＰ作为非线性晶体材料三阶非线性系数测量基准的可行性。     

Rarefied Flow Computations Using Nonlinear Model Boltzmann Equations

High resolution finite difference schemes for solving the nonlinear model Boltzmann equations are presented for the computations of rarefied gas flows. The discrete ordinate method is first applied to remove the velocity space dependency of the distribution function which renders the model Boltzmann equation in phase space to a set of hyperbolic conservation laws with source terms in physical space. Then a high order essentially nonoscillatory method due to Harten et al. (J. Comput. Phys. 71, 231, 1987) is adapted and extended to solve them. Explicit methods using operator splitting and implicit methods using the lower-upper factorization are described to treat multidimensional problems. The methods are tested for both steady and unsteady rarefied gas flows to illustrate its potential use. The computed results using model Boltzmann equations are found to compare well both with those using the direct simulation Monte Carlo results in the transitional regime flows and those with the continuum Navier-Stokes calculations in near continuum regime flows.     

不可压缩流的集中消失现象

分别对二维和三维不可压缩流体给出了一个较为直观的集中消失现象的充分条件,即若与近似解序列相应的弱*亏损测度所集中的集合在位置空间上的投影的Hausdorff维数小于1,则近似解序列在L²中的弱极限是Euler方程的经典弱解.利用这个充分条件,验证了一个集中的例子.