Construction of Post-column Micro-membrane Reactor for Protein Analysis in Capillary Electrophoresis with Laser Induced Fluorescence Detection

Proteomics is becoming more and more mature,but the detection of low abundance proteins is still a difficult task.Laser-induced fluorescence(LIF) detection is one of the most sensitive detection methods in a capillary electrophoresis(CE)system[1-3].However,most proteins do not exhibit favourable native fluorescence,a derivatization procedure is necessary for LIF detection of proteins.Since the derivatization reaction between protein and fluorescent reagent takes place after the separation of protein,the separation cannot be compromised by multiple derivatization products,the post-column derivatization becomes an attractive method for derivatization in CE-LIF system[4'5].The gap reactor,coaxial reactor and sheath flow cuvette reactor have been widely used for post-column derivatization in CE-LIF system[6].The gap reactor is constructed by aligning separation and reaction capillaries with a small gap[7,8].     

紫外像增强器信噪比与MCP电压的关系

日盲型紫外像增强器在空间光学探测、环境监测、战略国防等方面上具有非常广阔的应用前景,参照微光像增强器信噪比测试原理,设计了紫外像增强器信噪比测试系统,并给出其测试条件,利用研制的信噪比测试仪研究了微通道板两端电压对日盲型紫外像增强器信噪比的影响,测量了其关系曲线。结果表明,在小于850 V区域内,紫外像增强器的输出信噪比随着MCP两端电压的增大而增大;当超过850 V时,MCP两端电压增大,紫外像增强器的输出信噪比基本维持不变。     

Molecular Dynamics Study of Ionic Liquid Film Based on [emim][Tf2N] and [emim][TfO] Adsorbed on Highly Oriented Pyrolytic Graphite

Molecular dynamics simulation was used to study the ionic liquid(IL) crystalline film based on 1-ethyl-3-methylimidazolium bis[trifluoromethylsulfonyl]imide([emim][Tf₂N]) and 1-ethyl-3-methylimidazolium trifluoromethanesulfonate([emim][TfO]) on the graphite surface. Our results show that the cations are parallelly distributed to the surface in the 1/2 monolayer(ML) crystalline film. The [Tf₂N]^- anions are parallel to the surface with the oxygen atoms at the bottom, whereas the [TfO]^- anions are perpendicularly distributed to the surface also with the oxygen atoms at the bottom in the 1/2 ML crystalline film. It has been found that the IL-vapor interface strongly influences the arrangement of ions at the interface. The anions in the top layer with the oxygen atoms outmost turn over to make themselves with the F atoms outmost so as to form C-H···O hydrogen bonds with the cations. The calculated orientational ordering shows that in the outmost layer at the IL-vapor interface, the cation rings present either parallel or perpendicular to the surface at 350 K.     

高应力比循环下微动疲劳裂纹萌生位置的预测分析

对于微动疲劳问题,循环应力比的大小会影响试件应力状态及分布,从而影响疲劳裂纹的萌生位置.本文通过对一类微动疲劳问题进行有限元法分析,模拟疲劳实验过程,并采用最大应力变化幅△σθmax作为指标预测了不同应力比下疲劳裂纹的萌生位置.数值分析显示,在应力比不是很大时,试件与微动接触头的边缘存在接触,并在此处产生较大的应力集中,容易萌生裂纹;而在应力比足够高时,微动接触头端部与试件呈恒张开状态,△σθmax及裂纹萌生发生在距初始接触区边缘一定距离处.疲劳裂纹萌生位置的理论预测结果与相关试验的疲劳裂纹发生位置比较一致.     

单体结构对紫外光固化氧阻聚影响的研究

利用光量热仪(UV-DSC)对单体TMPTA以及不同乙氧基化的TMPTA单体进行了紫外光固化实验。通过比较空气气氛和氮气气氛下光固化速率增长最快时的时间t_amax的差异来评价光固化初期氧阻聚的大小。考察了单体中乙氧基上的α-H的数量以及单体的流动特性对氧阻聚的影响。结果表明,提高单体中乙氧基的数量能减小光固化过程中的氧阻聚,氧阻聚的大小与单体中α-H的数量以及单体的黏度有关.     

Calculation of prompt fission neutron spectra for 235U(n,f)

The prompt fission neutron spectra for the neutron-induced fission of ²³⁵U at E_n < 5 MeV are calculated using nuclear evaporation theory with a semi-empirical model, in which the nonconstant and constant temperatures related to the Fermi gas model are taken into account. The calculated prompt fission neutron spectra reproduce the experimental data well. For the n(thermal)+²³⁵U reaction, the average nuclear temperature of the fission fragment, and the probability distribution of the nuclear temperature, are discussed and compared with the Los Alamos model. The energy carried away by γ rays emitted from each fragment is also obtained and the results are in good agreement with the existing experimental data.     

青花菜抗坏血酸过氧化物酶基因BoAPX的克隆与表达分析

根据NCBI基因数据库提供的抗坏血酸过氧化物酶(Ascorbate Peroxidase,APX)基因序列设计简并引物,以青花菜(Brassica oleracea var.italica)叶片cDNA和DNA为材料进行PCR扩增,基因定名为BoAPX(Gen-Bank登录号:HQ871864).BoAPX的基因组全长为1 394bp,具7个内含子,编码区全长为753bp,编码250个氨基酸残基.序列分析结果表明,BoAPX的N端中没有信号肽序列,为胞质型APX,BoAPX与已知APX具有较高的同源性.RT-PCR结果表明,BoAPX的表达受霜霉菌(Hyaloperonospora parasitica)诱导,在96h时达最大值,说明BoAPX与霜霉菌抗性相关.以pBI121为植物表达载体,BoAPX为目的基因,成功构建了重组表达载体pBI121-BoAPX.     

Distributions and correlations of constituent quarks in jets

In the frame of the quark recombination model, we study the momentum distributions and cor- relations of constituent quarks in jets by analyzing the final state hadrons generated by PYTHIA for the hard parton fragmentation processes in vacuum. Parameterizations for the distributions are tabulated.     

2011 年软物质力学高级讲习班简介

由国家自然科学基金委员会数理科学部主办,中国力学学会、北京国际力学中心(BICTAM)协办, 浙江大学承办的软物质力学高级讲习班于2011 年12 月21 27 日在杭州浙江大学举办.      

多层开管毛细管酶反应器的制备及在蛋白质酶切中的应用

以石英毛细管作为酶固定化的载体, 在毛细管内壁上逐步合成树枝形大分子聚酰胺-胺(PAMAM), 再通过交联剂戊二醛将胰蛋白酶直接键合到该大分子的末端氨基上, 并对酶固定化条件进行了优化, 制备了多层酶反应器. 利用该酶反应器对马心细胞色素C等蛋白质进行了酶切, 并对酶切的条件进行了优化. 实验结果表明, 该固定化酶反应器具有较高的酶切效率、良好的重现性和稳定性, 可用于蛋白质组学的研究.