A Method for Measuring the Three-Dimensional Refractive-Index Distribution of Single Cells Using Proximal Two-Beam Optical Tweezers and a Phase-Shifting Mach—Zehnder Interferometer

This paper describes a method for measuring the three-dimensional (3D) refractive-index distribution in a single cell. The method can be used to observe the distribution of cell components without fluorescence staining. The two-dimensional optical path length distributions from multiple directions are obtained by non-contact rotation of the cell. These optical path lengths are converted into the line integrals of the refractive index, and the 3D refractive-index distribution is reconstructed by means of computed tomography. The refractive-index distribution in a breast cancer cell can be measured using a phase-shifting Mach—Zehnder interferometer in conjunction with proximal two-beam optical tweezers.     

Reduction of acoustic radiation by perforated board and honeycomb layer systems

A perforated system, proposed previously for reducing the radiated sound from a plate at arbitrary frequencies, is applied to three-dimensional problem. Plates are assumed to be supported in a duct of a finite cross-section and excited by a harmonic point force. The sound radiation is investigated from the viewpoint of acoustic power and it is discussed whether the attenuation effect shown previously in the one-dimensional system can be obtained with the three-dimensional system. The effect of support conditions on attenuation characteristics is discussed by using clamped and simply supported circular models. Allowing for the effect, a simply supported rectangular model is studied in detail and its problems are revealed. In order to overcome the problems, a new system including subdivided air cavities in the form of a honeycomb layer instead of a undivided backing cavity is proposed. Each of the honeycomb cells can create local one-dimensional sound fields. Calculated theoretical results are compared to data obtained in a 1/5th scale reverberation chamber. The results for the reduction effect, which are in good agreement, show that the honeycomb layer system can achieve the same reduction of the radiated sound power at arbitrary frequencies as the one-dimensional perforated system.     

Unsupervised Image Segmentation Using Hierarchical Clustering

In the analysis of a medical image database aimed at formulating useful knowledge for image diagnosis requires an unsupervised image processing technique without preconceived knowledge. In this paper, we propose a method for unsupervised image segmentation, which is suitable for finding the features contained in an image. A small region around each pixel is considered as a pattern vector, and the set of pattern vectors acquired from the whole image is classified using the hierarchical clustering technique. In hierarchical clustering, the classification of pattern vectors is divided into two clusters at each node according to the statistical criterion based on the entropy in thermodynamics. Results of the test image generated by the Markov random field (MRF) model and real medical images photomicrographs of a colon tumor are shown.     

Formation mechanism of element distribution in glass under femtosecond laser irradiation

We report on the formation mechanism of element distribution in glass under high-repetition-rate femtosecond laser irradiation. We simultaneously focused two beams of femtosecond laser pulses inside a glass and confirmed the formation of characteristically shaped element distributions. The results of the numerical simulation in which we considered concentration- and temperature-gradient-driven diffusions were in excellent qualitative agreement with the experimental results, indicating that the main driving force is the sharp temperature gradient. Since the composition of a glass affects its refractive index, absorption, and luminescence property, the results in this study provide a framework to fabricate a functional optical device such as optical circuits with a high-repetition-rate femtosecond laser.     

10 GHz, 2.4 ps pulse generation using a single-stage dual-drive Mach-Zehnder modulator

This Letter reports on picosecond pulse generation at a repetition of 10 GHz by using a single-stage electro-optic LiNbO(3) Mach-Zehnder modulator, stressing the simplicity of its setup. It is analytically and experimentally proved that dual-arm modulation with in-phase sinusoidal signals having slightly different amplitudes generated a highly coherent optical comb with a great spectral flatness and a parabolic phase relationship in its spectrum. The generated comb was Fourier synthesized and shaped into an ultrashort pulse train with an optical bandpass filter and a dispersive fiber. The pulse source was highly stable, exhibiting an ultralow timing jitter of less than 130 fs.     

A fundamental lower bound of actuating energy for broadband photon switching

We derive a universal lower bound of actuating energy Es for broadband photon switching by using an uncertainty relation between time and the negative energy density of quantum fields. We find that broadband photon switching between perfect reflection and perfect transmission over a time ts should satisfy .