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991.
Summary We have carried out up to 8.0 ns molecular dynamics simulation on the ATP-bound complexes of EGFR and HER-2 (homology model)
receptor kinase domains to explore the possible consequences of amino acid residue changes in or close to the ATP site that
might provide insights for selectivity of these kinases towards ATP site inhibitors. The simulation results show the formation
of a channel under Thr766 following the movement of the side chain of Gln767 away from the hinge in EGFR. In HER-2, a similar
movement of Gln799 occurs, but a simultaneous movement of Arg784 towards the hinge region occurs that tends to close the channel.
The movement of Arg784 in HER-2 appears to result from the absence of an anchoring residue like Asp746 in EGFR, which has
been changed to Gly778 in HER-2. In EGFR, this Arg784 is held away from the hinge region by interaction with Asp746, thereby
leaving the channel open. This might be an important contributory factor to differences in selectivity of the ligands between
the two kinases, probably more so than the conservative change of Cys751 of EGFR to serine in HER-2 at the ATP site. 相似文献
992.
Baeten V von Holst C Garrido A Vancutsem J Michotte Renier A Dardenne P 《Analytical and bioanalytical chemistry》2005,382(1):149-157
In this paper we present an alternative method for detection of meat and bone meal (MBM) in feedstuffs by near-infrared microscopic (NIRM) analysis of the particles in the sediment fraction (dense fraction (d >1.62) from dichloroethylene) of compound feeds. To apply this method the particles of the sediment fraction are spread on a sample holder and presented to the NIR microscope. By using the pointer of the microscope the infrared beam is focussed on each particle and the NIR spectrum (1112–2500 nm) is collected. This method can be used to detect the presence of MBM at concentrations as low as 0.05% mass fraction. When results from the NIRM method were compared with the classical microscopic method, a coefficient of determination (R2) of 0.87 was obtained. The results of this study demonstrated that this method could be proposed as a complementary tool for the detection of banned MBM in feedstuffs by reinforcement of the monitoring of feeds. 相似文献
993.
Yu JC Krushkova S Lai EP Dabek-Zlotorzynska E 《Analytical and bioanalytical chemistry》2005,382(7):1534-1540
A molecularly-imprinted polymer (MIP) was prepared by electropolymerization of pyrrole (Py) onto a stainless steel frit, using ochratoxin A (OTA) as the template, in order to make a micro solid phase preconcentration (SPP) device. The OTA template was removed with 1% triethylamine (TEA) in methanol. Compared to non-imprinted polypyrrole (PPy), the molecularly-imprinted polypyrrole (MIPPy) enhanced the selective binding of OTA. The percentage recovery improved from 0 to 40% when the OTA sample solution was acidified with 1 M HCl (1% by volume). At a flow rate of 0.2 mL/min, maximum OTA binding was reached in 6 min after a total loading of 3.2 ng OTA. Final elution of the OTA was analyzed by high performance liquid chromatography (HPLC) with fluorescence detection, using 20:80 v/v acetonitrile–ammonia buffer (NH4Cl/NH3, 20 mM, pH 9.2) as the mobile phase. The MIPPy-SPP-HPLC results clearly demonstrated that the MIPPy-SPP device afforded selective preconcentration of OTA from red wine samples, at OTA concentration levels as low as 0.05 ppb, prior to HPLC analysis. 相似文献
994.
Sandra Hübner Dirk Michalik Dr. Haijun Jiao Dr. Helfried Neumann Dr. Stefan Klaus Dirk Strübing Dr. Anke Spannenberg Dr. Matthias Beller Prof. Dr. 《化学:亚洲杂志》2007,2(6):734-746
Chiral N‐dienyl lactams are crucial building blocks for the synthesis of complex organic compounds. However, their generation is rather challenging. This paper reports the novel one‐pot reaction of (S)‐methyl pyroglutamate as the a mide component with different a ldehydes and d ienophiles (AAD reaction) to give novel chiral 1‐amido‐2‐cyclohexenes. The corresponding N‐dienyl lactams generated in situ undergo subsequent Diels–Alder reactions in good yield and diastereoselectivity. The scope and limitations of the three‐component protocol were investigated. X‐ray and NMR spectroscopic analysis of the products as well as DFT calculations of the intermediates were also performed to explain the observed stereoselectivity and structural features. 相似文献
995.
Frank?WendlerEmail author Gerhard?Grane? Thomas?Heinze 《Cellulose (London, England)》2005,12(4):411-422
The thermal stability of cellulose/N-methylmorpholine-N-oxide (NMMO) solutions were investigated using UV/VIS spectrometry with a temperature programming cuvette and caloric measurements
by means of the Systag calorimeter RADEX (mini-autoclave). Both analytical methods allow to characterize the influences of
stabilizers and additives. With the temporal course of the optical density, temperature and pressure thermal runaway reactions
with gas evolution and accumulation of chromophoric degradation products were recognized. Kinetic model calculations compared
with UV/VIS measurements demonstrate the existence of autocatalytic reactions in cellulose/NMMO solutions. Varying the heating
rate autocatalysis can be proved by dynamic caloric measurements as well. 相似文献
996.
Shigeru?ArimotoEmail author Mark?Spivakovsky Keith?F.?Taylor Paul?G.?Mezey 《Journal of mathematical chemistry》2005,37(2):171-189
The present article is a direct continuation of the first part of this series. We reduce a proof of the Fukui conjecture (concerning the additivity problem of the zero-point vibrational energies of hydrocarbons) to that of a proposition related to the theory of algebraic curves, so that we can focus on the key mechanism of the additivity phenomena. Namely, by establishing what is called the Basic Piecewise Monotone Theorem (BPMT), we reduce a proof of the Fukui conjecture to that of a proposition, called the Local Analyticity Proposition, Version 1 (LAP1), which admits a proof via resolution of singularities. By LAP1, the essential part of the mechanism of the asymptotic linearity phenomena is extracted and is elucidated by using tools from the mathematical theory of algebraic curves, whose language is of vital importance in analyzing the crux of the additivity mechanism.
Dedicated to the memory of Prof. Kenichi Fukui (1918–1998). 相似文献
997.
Protease sensors for bioimaging 总被引:2,自引:0,他引:2
Optical imaging of specific molecular targets and pathways in vivo has recently become possible through continued developments in imaging equipment, reconstruction algorithms, and more importantly the availability of imaging reporter molecules. These reporter molecules encompass photoproteins expressed in vivo and exogenously administered probes detectable by fluorescence and/or bioluminescence imaging. One particularly enticing aspect of optical imaging is the ability to design activatible probes with inherent amplification. This review summarizes our experience in developing novel near-infrared fluorescent (NIRF) imaging agents that report on protease activities. These agents are designed to be biocompatible, highly activatible, and able to produce bright NIRF following protease cleavage. 相似文献
998.
Macrodefect-free (MDF) materials are one example of “hot topics” in the field of cement-based materials and composites exerting
new possibilities of the exploitation of added value. These are formed through cross-linking reactions of atoms at the interfaces
of cement grains and functional polymers, when medium pressure and twin-rolling procedure are applied. The MDF-relevance of
the system of Portland cement + polyphosphate is reported, together with optimal synthesis conditions and limiting rules.
The chemistry knowledge about MDF materials has been shown critical for both procedure design and exploitation. Chemical shifts
in both 27Al and 31P MAS NMR spectra confirm Al(6)—O—P(4) cross-linking in virgin probes and indicate secondary hydrolysis during moisture uptake
in domains free of cross-links. Thermogravimetric identification of the contents of hydrated and cross-linked phases in virgin
and in moisture-attacked MDF probes displays that moisture uptake is accompanied by an increase in content of cementitious
hydrates and CaCO3. The key phenomena governing the moisture sensitivity/resistance are the density and compactness of interfacial Al(6)—O—P(4)
cross-links vs. the access of the moist environment to the unreacted cement residue.
The paper was presented at the 20 ICCBiC (International Conference on Coordination and Bioinorganic Chemistry) held on 5–10
June 2005 in Smolenice, Slovakia. 相似文献
999.
Neringa Kleizien? Virgin? Amankavi?ien? Ulf Berg Carsten Schicktanz Klaus Schlothauer Algirdas ?a?kus 《Monatshefte für Chemie / Chemical Monthly》2006,43(9):1109-1117
Condensation of 1-substituted 1,2,3,9a-tetrahydro-9H-imidazo[1,2-a]indol-2-ones with 5-nitrosalicylaldehyde afforded 1′-[(N-monosubstituted carbamoyl)methyl]indoline nitrospirobenzopyrans.
Treatment of the latter with strong base led to the formation of a mixture of cis/trans-5a,13-methano-1,3-benzoxazepino[3,2-a]indoles. Results of semiempirical calculations gave evidence that such a transformation of nitrospirobenzopyrans to bicyclic
indole derivatives could proceed via a single transition state, where the negatively charged carbon atom attacks the vinylic double bond of the spiropyran system. 相似文献
1000.
Summary The inhibitory effect on cholinesterase activity is the only common attribute of organophosphorus pesticides and carbamates. Therefore a screening test based on cholinesterase assay is suitable for detection of total pesticides in the pollution control of surface waters. The reaction-rate cholinesterase assay in which acetylthiocholine iodide is used as substrate and 5,5-dithio-bis (2-nitrobenzoic acid) (Ellman's reagent) as indicator, with spectrophotometric monitoring at 412 nm for 2 min is applied. The detection limits for various pesticides in aqueous solutions have been determined. The results are comparable to those of gas Chromatographic analyses and for carbamates are even better. The sensitivity can be improved by two orders of magnitude by preconcentration on Amberlite XAD-4 column. The collection efficiency of about 80% for all the pesticides tested has been confirmed by gas chromatography. A rapid and simple field test is based on the same assay system. The reaction is stopped by complete inhibition of the enzyme and the colour is compared visually with that of standards prepared by dilution of the initial enzyme solution, and the comparison is facilitated by use of a red filter (max 555 nm). A relative standard deviation of 5.5% was obtained for 3 series of sample covering the range 0–100% inhibition, 9 experiments done by 6 persons.
Presented at the 8th International Microchemical Symposium, Graz, August 25–30, 1980. 相似文献
Nachweis von Spuren Cholinesterase hemmender Faktoren in Oberflächenwässern
Zusammenfassung Der hemmende Effekt gegenüber Cholinesterase ist das einzige gemeinsame Kennzeichen phosphor-organischer Pestizide und Carbamate. Der Nachweis mit Cholinesterase eignet sich daher für den Nachweis von Pestiziden bei der Reinheitskontrolle von Oberflächengewässern. Der Nachweis mit Acetylthiocholinjodid als Substrat und 5,5-Dithio-bis(2-nitrobenzoe-säure) (Ellmans Reagens) als Indikator durch spektrophotometrische Bestimmung bei 412 nm wurde angewendet. Die Erfassungsgrenzen für verschiedene Pestizide in wäßriger Lösung wurden bestimmt. Die Ergebnisse sind mit denen gaschromatographischer Analysen vergleichbar, für Carbamate sogar besser. Die Empfindlichkeit kann durch Anreicherung mit Amberlit XAD-4 um zwei Größenordnungen verbessert werden. Die Anreicherung um ungefähr 80% wurde gaschromatographisch für alle untersuchten Pestizide bestätigt. Ein schneller und einfacher Feldversuch beruht auf demselben Reaktionsvorgang. Die Reaktion wird durch vollständige Hemmung des Enzyms abgebrochen und die Farbe visuell verglichen mit verdünnten Standardlösungen der ursprünglichen Enzymlösung. Dieser Vergleich wird durch ein Rotfilter (max 555 nm) erleichtert. Eine relative Standardabweichung von 5,5% wurde für 3 Probenreihen mit einem Hemmungseffekt von 0–100% erhalten, wobei 6 Personen 9 Versuche durchführten.
Presented at the 8th International Microchemical Symposium, Graz, August 25–30, 1980. 相似文献