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Esra Sengul Pouya Sharbati Meltem Elitas Monsur Islam Jan G. Korvink 《Electrophoresis》2022,43(12):1357-1365
Glioblastoma multiforme is the most aggressive and invasive brain cancer consisting of genetically and phenotypically altering glial cells. It has massive heterogeneity due to its highly complex and dynamic microenvironment. Here, electrophysiological properties of U87 human glioma cell line were measured based on a dielectrophoresis phenomenon to quantify the population heterogeneity of glioma cells. Dielectrophoretic forces were generated using a gold-microelectrode array within a microfluidic channel when 3 Vpp and 100, 200, 300, 400, 500 kHz, 1, 2, 5, and 10 MHz frequencies were applied. We analyzed the dielectrophoretic behavior of 500 glioma cells, and revealed that the crossover frequency of glioma cells was around 140 kHz. A quantifying dielectrophoretic movement of the glioma cells exhibited three distinct glioma subpopulations: 50% of the glioma cells experienced strong, 30% of the cells were spread in the microchannel by moderate, and the rest of the cells experienced very weak positive dielectrophoretic forces. Our results demonstrated the dielectrophoretic spectra of U87 glioma cell line. Dielectrophoretic responses of glioma cells linked population heterogeneity to membrane properties of glioma cells rather than their size distribution in the population. 相似文献
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A computational technique based on the Müntz polynomials and meshless method has been presented for the solution of nonlinear and linear space fractional partial differential equations (PDEs). The meshless method that is used in this study is the new version of backward substitution method (BSM). First, the time-derivative term is discretized by the Crank-Nicolson method. Then, the approximate solution is given as the separation of the approximation of the boundary data and the correcting functions using the Müntz polynomials. In general form, this approximate solution that does not necessarily satisfy the original equation is shown as the sum of the system basics in which it consists free parameters. Finally, these parameters are determined by the BSM method inside the domain. The main advantage of the method is efficiency and reliability that is examined by six numerical experiments. 相似文献
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Oveisi Ali Reza Karimi Pouya Delarami Hojat Samareh Daliran Saba Khorramabadi-zad Ahmad Khajeh Mostafa Sanchooli Esmael Ghaffari-Moghaddam Mansour 《Molecular diversity》2020,24(2):335-344
Molecular Diversity - New trans-A2B2-porphyrins substituted at phenyl positions were synthesized from 4-methylphthalic acid as a starting material through sequential multistep reactions. These... 相似文献
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The excitation function for the fission of 230Th induced by neutrons has an unusual maximum for neutron energies in the vicinity of 700KeV,It has been suggested that this maximum may be associated with the vibrational-mode resonance states,The unusual peak in the excitation function is interpreted in terms of a vibrational-model resonance state in a two-humpted fission barrier.From theoretical fits to the fission cross sections and angular distributions,it is shown that the resonance has K=1/2. 相似文献
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Abtahi Seyed Mohammad Mahdi Kargar Shaker Langaroodi Roghayeh Akbari Mohammad Esmail 《Journal of Radioanalytical and Nuclear Chemistry》2020,324(2):481-488
The purpose of the current study was to verify the dose distribution of an Intrabeam-50 kV IORT system using polymer gel dosimetry technique. Results of dose distribution evaluation using NIPAM polymer gel dosimetry were compared with those measured using an ionization chamber and simulated using MCNPX code. Results showed the calculated gamma index was less than 1 with 2% dose-difference/2 mm distance-to-agreement for comparison between NIPAM and ionization chamber as well as between NIPAM and MCNPX simulation. It was concluded that the NIPAM polymer gel dosimetry is useful for verifying the dose distribution of low energy X-ray IORT technique.
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