Ring-opening reactions of N-methyl cyclic ketene-N,O-acetals with carboxylic acids, nitrophenol, and arylthiols

Ring-opening reactions of N-methyl cyclic ketene-N,O-acetals with carboxylic acids, 4-nitrophenol, and arylthiols afforded amidoesters, amidoaryl ether, and amidothioethers, respectively, in good yields via an acid-catalyzed S(N)2 mechanism.     

Smart solution chemistry: Prolonging the lifetime of ortho‐phthalaldehyde disinfection solutions

The oxidation of ortho‐phthalaldehyde to phthalic acid in aqueous solutions can be remedied by the addition of various cyclic acetals, which, when reacted with phthalic acid, yields ortho‐phthalaldehyde, thus prolonging the lifetime of the ortho‐phthalaldehyde disinfection solution.     

On-line monitoring of electroosmotic flow for capillary electrophoretic separations

A recently developed technique for monitoring electroosmotic flow (EOF) in capillary electrophoresis by periodic photobleaching of a neutral fluorophore added to the running buffer has been further characterized and optimized and then applied to monitoring EOF during a typical capillary electrophoresis separation. The concentration of neutral fluorophore (rhodamine B) added to the running buffer for monitoring EOF has been decreased by one order of magnitude. The rate at which EOF can be measured has been increased from 0.2 to 1.0 Hz by decreasing the distance between the bleaching beam and the laser-induced fluorescence detector from 6.13 to 0.635 mm. The precision of the measured EOF ranges from 0.2 to 1.8%. Under typical experimental conditions, the dynamic range for flow measurements is 0.066 to 0.73 cm s(-1). Experimental factors affecting precision, signal-to-noise (S/N) ratio and dynamic range for EOF monitoring have been examined. This technique has been applied to measure EOF during a separation of phenolic acids with analyte detection by UV/VIS absorbance. The EOF monitoring method has been shown not to interfere with UV/VIS absorbance detection of analytes.     

Hybrid inorganic/organic crosslinked resins containing polyhedral oligomeric silsesquioxanes

The incorporation of both monofunctional and multifunctional polyhedral oligomeric silsesquioxane (POSS) derivatives into crosslinked resins has been conducted as a route to synthesize hybrid organic/inorganic nanocomposites. The central cores of POSS molecules contain an inorganic cage with (SiO_1.5)_n stoichiometry where n=8,10 and 12. Each Si atom is capped with one H or R function giving an organic outer shell surrounding the nanometer-sized inorganic inner cage. By including polymerizable functions on the R groups, a hybrid organic/inorganic macromer is obtained which can be copolymerized with organic monomers to create thermoplastic or thermoset systems. We have focused on incorporating POSS derivatives into crosslinking resins of the following types: (1) dicyclopentadiene (2) epoxies (3) vinyl esters (4) styrene-DVB (5) MMA/1,4-butane dimethacrylate (6) phenolics and (7) cyanate esters. One goal has been to determine if molecular dispersion of the POSS macromers has been achieved or if various degrees of aggregation occur during crosslinked resin formation. As network formation proceeds, a kinetic race between POSS molecular incorporation into the network versus phase separation into POSS-rich regions (which then polymerize) occurs. Ultimately, we hope to determine the effects of such microstructural features on properties. Combustion of these hybrids creates a SiO₂-like surface layer that retards flame spread. Dynamic mechanical properties have been studied.     

Raman spectral analysis of renal tissue: a novel application

Renal cell carcinoma (RCC) accounts for 85% of all primary renal cancers. The definitive diagnosis of RCC relies exclusively on the subjective pathological interpretation of the surgical specimen. In this study, we aimed to analyze renal tissue using objective Raman spectroscopy (RS). We obtained 15 pairs of RCC (T) and corresponding normal renal parenchymal tissues (N) from our biobank. There are three subtypes of RCC: clear cell RCC (ccRCC), papillary RCC (pRCC), and chromophobe RCC (cRCC). Five pairs of tissue of each subtype were enrolled. Fresh‐frozen sliced tissues were used for the RS detection. The Raman spectra between T and N were compared and analyzed using partial least squares (PLS) regression. Data for a total of 55 T and 58 N analyzable RS samples were obtained. The spectra were normalized by dividing the intensity of the characteristic peak at 1003 cm⁻¹ using phenylalanine's Raman peak. After further analysis with PLS, the sensitivity and specificity for discriminating T from N were 95% and 93%, respectively. The RCC subtypes can be discriminated at an accuracy of 72% for ccRCC, 88% for cRCC, and 86% for pRCC. This study demonstrates the feasibility of analyzing renal tissue using RS. RS, with its advantages of easy and objective tissue assessment, may be applied to aid intraoperative decision making and pathological tissue assessment. Copyright © 2014 John Wiley & Sons, Ltd.