Variable phase dark-field contrast--a variant illumination technique for improved visualizations of transparent specimens

Variable phase dark-field contrast has been developed as an illumination technique in light microscopy, which promises significant improvements and a higher variability in imaging of several transparent specimens. In this method, a phase contrast image is optically superimposed on a dark-field image so that a partial image based on the principal zeroth-order maximum (phase contrast) interferes with an image that is based on the secondary maxima (dark field). The background brightness and character of the resulting image can be continuously modulated from a phase-contrast-dominated to a dark-field-dominated character. The condenser aperture diaphragm can be used for modulations of the image's appearance. Specimens can either be illuminated concentrically or obliquely (eccentrically) when parts of the illuminating light beams are covered and blocked. Moreover, a bright-field-like partial image can be added. In this way, the illumination can be optimally adjusted to the specific properties of the specimen. The techniques described can lead to improved visual information especially in biological specimens consisting of phase structures and additional light-absorbing or -reflecting components. Moreover, the specimen's three-dimensionality can be accentuated with improved clarity because the illuminating light beams associated with phase contrast and dark field run to the specimen at different angles of incidence.     

Optical activity of trigonal coordination compounds

The optical activity of trigonal coordination compounds is investigated using the crystal field theory. The ionic model is shown to account for the circular dichroism of Co(en)₃ ⁺³. The absolute configuration of Cr(C₂O₄)₃ ^-3 is assigned on the basis of the ionic model.     

A continuous wave SrMoO4 Raman laser

We demonstrate a cw, laser diode-pumped Nd:GdVO4/SrMoO4 crystalline Raman laser. First Stokes laser output at 1173.5?nm of 2.18?W was achieved with a diode-to-first Stokes efficiency of 8.7%. With intracavity frequency doubling in LiB3O5, 3.1?W of cw yellow emission at 586.8?nm was obtained with a 12.4% diode-to-yellow efficiency. The experimental results show that SrMoO4 is an excellent stimulated Raman scattering gain material for high-power cw near-IR Stokes and yellow lasers.     

Effect of changes in the deuterium content of drinking water on the hydrogen isotope ratio of urinary steroids in the context of sports drug testing

The hydrogen isotope ratio (HIR) of body water and, therefore, of all endogenously synthesized compounds in humans, is mainly affected by the HIR of ingested drinking water. As a consequence, the entire organism and all of its synthesized substrates will reflect alterations in the isotope ratio of drinking water, which depends on the duration of exposure. To investigate the effect of this change on endogenous urinary steroids relevant to doping-control analysis the hydrogen isotope composition of potable water was suddenly enriched from -50 to 200 ‰ and maintained at this level for two weeks for two individuals. The steroids under investigation were 5β-pregnane-3α,20α-diol, 5α-androst-16-en-3α-ol, 3α-hydroxy-5α-androstan-17-one (ANDRO), 3α-hydroxy-5β-androstan-17-one (ETIO), 5α-androstane-3α,17β-diol, and 5β-androstane-3α,17β-diol (excreted as glucuronides) and ETIO, ANDRO and 3β-hydroxyandrost-5-en-17-one (excreted as sulfates). The HIR of body water was estimated by determination of the HIR of total native urine, to trace the induced changes. The hydrogen in steroids is partly derived from the total amount of body water and cholesterol-enrichment could be calculated by use of these data. Although the sum of changes in the isotopic composition of body water was 150 ‰, shifts of approximately 30 ‰ were observed for urinary steroids. Parallel enrichment in their HIR was observed for most of the steroids, and none of the differences between the HIR of individual steroids was elevated beyond recently established thresholds. This finding is important to sports drug testing because it supports the intended use of this novel and complementary methodology even in cases where athletes have drunk water of different HIR, a plausible and, presumably, inevitable scenario while traveling.     

Metabolism of androsta‐1,4,6‐triene‐3,17‐dione and detection by gas chromatography/mass spectrometry in doping control

The urinary metabolism of the irreversible aromatase inhibitor androsta‐1,4,6‐triene‐3,17‐dione was investigated. It is mainly excreted unchanged and as its 17β‐hydroxy analogue. For confirmation, 17β‐hydroxyandrosta‐1,4,6‐trien‐3‐one was synthesized and characterized by nuclear magnetic resonance (NMR) in addition to the parent compound. In addition, several reduced metabolites were detected in the post‐administration urines, namely 17β‐hydroxyandrosta‐1,4‐dien‐3‐one (boldenone), 17β‐hydroxy‐5β‐androst‐1‐en‐3‐one (boldenone metabolite), 17β‐hydroxyandrosta‐4,6‐dien‐3‐one, and androsta‐4,6‐diene‐3,17‐dione. The identification was performed by comparison of the metabolites with reference material utilizing gas chromatography/mass spectrometry (GC/MS) of the underivatized compounds and GC/MS and GC/tandem mass spectrometry (MS/MS) of their trimethylsilyl (TMS) derivatives. Alterations in the steroid profile were also observed, most obviously in the androsterone/testosterone ratio. Even if not explicitly listed, androsta‐1,4,6‐triene‐3,17‐dione is classified as a prohibited substance in sports by the World Anti‐Doping Agency (WADA) due to its aromatase‐inhibiting properties. In 2006 three samples from human routine sports doping control tested positive for metabolites of androsta‐1,4,6‐triene‐3,17‐dione. The samples were initially found suspicious for the boldenone metabolite 17β‐hydroxy‐5β‐androst‐1‐en‐3‐one. Since metabolites of androst‐4‐ene‐3,6,17‐trione were also present in the urine samples, it is presumed that these findings were due to the administration of a product like ‘Novedex Xtreme’, which could be easily obtained from the sport supplement market. Copyright © 2008 John Wiley & Sons, Ltd.     

Determination of the deuterium/hydrogen ratio of endogenous urinary steroids for doping control purposes

The development and application of a combined gas chromatography/thermal conversion/isotope ratio mass spectrometry (GC/TC/IRMS) method for D/H ratio determination of endogenous urinary steroids are presented. The key element in sample preparation was the consecutive cleanup with high‐performance liquid chromatography of initially native and subsequently acetylated steroids. This strategy enabled sufficient cleanup off all target analytes for determination of their respective D/H values. Ten steroids (11β‐hydroxyandrosterone, 5α‐androst‐16‐en‐3α‐ol, pregnanediol, androsterone, etiocholanolone, testosterone, epitestosterone, 5α‐androstan‐3α,17β‐diol, 5β‐androstan‐3α,17β‐diol and dehydroepiandrosterone) were measured from a single urine specimen. Depending on the biological background, the determination limit for all steroids ranged from 10 to 15 ng/mL for a 20 mL specimen. The method was validated by application of linear mixing models on each steroid and covered repeatability and reproducibility. The specificity of the procedure was ensured by gas chromatography/mass spectrometry (GC/MS) analysis of the sample using equivalent chromatographic conditions to those employed in the GC/TC/IRMS measurement. Within the sample preparation, no isotopic fractionation was observed, and no amount‐dependent shift of the D/H ratios during the measurement was noticed. Possible memory effects occurring during IRMS measurements were corrected by applying a simple rule of proportion. In order to determine the naturally occurring D/H ratios of all implemented steroids, a population of 18 male subjects was analyzed. Relevant mean Δ values among selected steroids were calculated which allowed us to study the metabolic pathways and production sites of all the implemented steroids with additional consideration of the corresponding ¹³C/¹²C ratios. Copyright © 2009 John Wiley & Sons, Ltd.     

Consonant recognition in quiet and in noise with aging among normal hearing listeners

Consonant recognition in quiet and in noise was investigated as a function of age for essentially normal hearing listeners 21-68 years old, using the nonsense syllable test (NST) [Resnick et al., J. Acoust. Soc. Am. Suppl. 1 58, S114 (1975)]. The subjects audited the materials in quiet and at S/N ratios of +10 and +5 dB at their most comfortable listening levels (MCLs). The MCLs approximated conversational speech levels and were not significantly different between the age groups. The effects of age group, S/N condition (quiet, S/N +10, S/N +5) and NST subsets, and the S/N condition X subset interaction were all significant. Interactions involving the age factor were nonsignificant. Confusion matrices were similar across age groups, including the directions of errors between the most frequently confused phonemes. Also, the older subjects experienced performance decrements on the same features that were least accurately recognized by the younger subjects. The findings suggest that essentially normal older persons listening in quiet and in noise experience decreased consonant recognition ability, but that the nature of their phoneme confusions is similar to that of younger individuals. Even though the older subjects met the same selection criteria as did younger ones, there was an expected shift upward in auditory thresholds with age within these limits. Sensitivity at 8000 Hz was correlated with NST scores in noise when controlling for age, but the correlation between performance in noise and age was nonsignificant when controlling for the 8000-Hz threshold. These associations seem to implicate the phenomena underlying the increased 8000-Hz thresholds in the speech recognition problems of the elderly, and appear to support the concept of peripheral auditory deterioration with aging even among those with essentially normal hearing.