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71.
Luane Ferreira Garcia Edson Silvio Batista Rodrigues Guilherme Rocha Lino de Souza Isabela Jub Wastowski Fernando Mota de Oliveira Wallans Torres Pio dos Santos Eric de Souza Gil 《Electroanalysis》2020,32(5):1100-1106
The bovine herpesvirus type 1 (BHV‐1) is a pathogen of great economic impact for livestock, which is related multi‐systemic infections that leads to mortality or morbidity of cattles. Thus, the search for cheap and practical methodologies that allow the selective detection of BHV‐1 antigen (BHV‐1 AG) is of utmost relevance. Therefore, an impedimetric label‐free immunosensor was herein, developed and its performance evaluated in biological samples enriched with BHV‐1 AG. Briefly, the biosensor construction was based on the immobilization of BHV‐1 antibody (BHV‐1 AB) and casein on the activated glassy carbon electrode surface. The BHV‐1 AB was isolated from egg yolk of immunized chickens, which is a less stressful protocol. The bio sensing principle was based on Electrochemical Impedance Spectroscopy by using Fe(CN)64?/3? probe, which were also used to check variation of charge transfer resistance (?Rct), when the electrode surface was increasingly blocked by immune complex. A linear relationship between ?Rct and BHV‐1 AG concentration was verified in the range from 10 to 50 TCID50 mL?1, with LOQ of 2.00 TCID50 mL?1 and LOD of 0.66 TCID50 mL?1. Besides the suitable sensitivity, the immunosensor displayed accuracy, stability, and specificity to detect BHV‐1 AG in biological samples of serum, nasal secretions, semen and urine. Moreover, to the best of our knowledge this is the first immunosensor applied to BHV‐1 diagnostic. 相似文献
72.
Victor Hugo Catricala Fernandes Natalie de Barros Viera Luiza Beatriz Lima Zanini Aline de Fátima Silva Paula Pio de Oliveira Salem Marisi Gomes Soares Karen de Jesus Nicácio Ana Claudia Chagas de Paula Luciano Sindra Virtuoso Tiago Branquinho Oliveira Eliane de Oliveira Silva Danielle Ferreira Dias Daniela Aparecida Chagas-Paula 《Photochemistry and photobiology》2020,96(1):14-20
There is some evidence in the literature of the photocyclization reaction of Tagitinin C ( 1 ) to Tagitinin F ( 2 ). Compound 2 has high pharmacological potential, but it is not easy to obtain, while compound 1 is easily obtained from a widespread plant, Tithonia diversifolia. Among different reaction conditions monitored, one was found that allowed the cyclization of 1 into 2 in <15 min in a photo-dependent reaction. Scaling-up the photocyclization of the pure compound 1 into 2 demonstrated 100% yield, and the isolation of 2 from a UV-irradiated extract was eight-fold higher than the quantity isolated from the non-UV-irradiated extract. We were also able to better understand the process of photoconversion and determine methods to isolate and quantify these compounds, which are known for their important antitumoral activities among other important pharmacological properties. 相似文献
73.
Hans A. V. Kistemaker Dr. Aurelio Pio Nardozza Prof. Dr. Herman S. Overkleeft Prof. Dr. Gijs A. van der Marel Prof. Dr. Andreas G. Ladurner Dr. Dmitri V. Filippov 《Angewandte Chemie (International ed. in English)》2016,55(36):10634-10638
Mono‐ADP‐ribosylation is a dynamic posttranslational modification (PTM) with important roles in signaling. Mammalian proteins that recognize or hydrolyze mono‐ADP‐ribosylated proteins have been described. We report the synthesis of ADP‐ribosylated peptides from the proteins histone H2B, RhoA and, HNP‐1. An innovative procedure was applied that makes use of pre‐phosphorylated amino acid building blocks. Binding assays revealed that the macrodomains of human MacroD2 and TARG1 exhibit distinct specificities for the different ADP‐ribosylated peptides, thus showing that the sequence surrounding ADP‐ribosylated residues affects the substrate selectivity of macrodomains. 相似文献
74.
Maria M. Giangregorio Maria Losurdo Alberto Sacchetti Pio Capezzuto Giovanni Bruno 《Journal of luminescence》2006,121(2):322-326
Au nanoclusters have been deposited on Si(0 0 1) surfaces by sputtering of a metallic Au target using an Ar plasma. Different wet and dry treatments of the Si(0 0 1) surface, including dipping in HF solution and exposure to H2 and N2 plasmas, have been applied and the effects of these treatments on the Au nanoparticles/Si interface, the Au nanoclusters aspect ratio and the surface plasmon resonance (SPR) energy and amplitude are investigated exploiting spectroscopic ellipsometry and atomic force microscopy. It is found that the Au nanoclusters aspect ratio depends on the extent of the Au-Si intermixing. The thicker the Au-Si interface layer, the larger the Au nanoparticles aspect ratio and the red-shift of the SPR peak. Furthermore, SiO2 and the H2 plasma treatment inhibit the Si-Au intermixing, while HF-dipping and the N2 plasma treatment favour Au-Si intermixing, yielding silicide formation which increases the Si wetting by Au. 相似文献
75.
Roberto Napolitano Beniamino Pirozzi Pio Iannelli 《Macromolecular theory and simulations》2001,10(9):827-832
The crystal structure of poly(p‐phenylene sulfide) (PPS) has been investigated by X‐ray analysis on fiber samples and by molecular mechanics calculations over a wide range of temperatures, from 0 K to 548 K, showing that all the structural parameters remain substantially constant. The thermal expansion coefficients of the a and b axes have been evaluated. Structural parameters experimentally obtained at the various temperatures have been used in calculations by molecular mechanics. The crystal structures calculated by various methods and using several potential functions are in very good agreement. New parameters are proposed for the nonbonded terms of the potential functions. 相似文献
76.
Luciano Di Maio Pio Iannelli Stefania Pragliola Antonio Roviello Augusto Sirigu 《Journal of Polymer Science.Polymer Physics》1998,36(3):433-438
Preliminary results on the synthesis and characterization of anisotropic networks, oriented on a macroscopic scale, are reported. Fiber samples of segmented thermotropic liquid-crystalline polymers bearing the oxypentenyl lateral substituent have been crosslinked via thermally activated radical reaction. This was made possible by immersion of fiber samples in dichloromethane containing t-butylperoxybenzoate as activating agent, thus allowing its diffusion in the samples. Subsequent annealing at 145°C brings us to an anisotropic network with no loss of the original orientation. A mesophase is stabilized and no structural modification is observed by heating samples from room temperature up to 400°C, where thermal decomposition takes place. Crosslinked fibers exhibit good tensile properties, at both room temperature and at 150°C. © 1998 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 36: 433–438, 1998 相似文献
77.
The melting point depression of polymer-solvent mixtures as a function of the concentration has been measured by DSC methods for a group of semiflexible linear mesophasic polymers of formula The molar enthalpic changes associated with the phase transitions have been determined. The values of the ratio between the molar isotropization entropy and the total molar entropic change relative to the sequence of phase transitions leading from the crystal phase to the isotropic liquid have been taken into account as an indication of the order of the mesophase. A particularly high value of this ratio has been found for the even members of the homologous series of polymers. 相似文献
78.
The composition of the water-soluble organic matter from fine aerosols collected in a rural location during two different meteorological conditions (summer and autumn) was investigated by UV-vis, synchronous fluorescence (with Δλ = 20 nm), FT-IR and CPMAS-13C NMR spectroscopies. A seasonal variation in the concentration of total carbon, organic carbon and water-soluble organic carbon was confirmed, with higher values during the autumn and lower values during the summer season. The chemical characterisation of the water-soluble organic matter showed that both samples are dominated by a high content of aliphatic structures, carboxyl groups and aliphatic carbons single bonded to one oxygen or nitrogen atom. However, the autumn sample exhibits a higher aromatic content than the summer sample, plus signals due to carbons of phenol, ketones and methoxyl groups. These signals were attributed to lignin breakdown products which are likely to be released during wood combustion processes. The obtained results put into evidence the major contribution of biomass burning processes in domestic fireplaces during low temperature conditions into both the concentration and the bulk chemical properties of the WSOC from fine aerosols. 相似文献
79.
80.
Clemenza Massimiliano Bonesini Maurizio Carpinelli Massimo Cremonesi Oliviero Fiorini Ettore Gorini Giuseppe Hillier Adrian Ishida Katsu Menegolli Alessandro Mocchiutti Emiliano Oliva Piernicola Prata Marco Rendeli Marco Rignanese Luigi Pio Rossella Massimo Sipala Valeria Soldani Mattia Tortora Ludovico Vacchi Andrea Vallazza Erik 《Journal of Radioanalytical and Nuclear Chemistry》2019,322(3):1357-1363
Journal of Radioanalytical and Nuclear Chemistry - The implementation in the RIKEN-RAL negative muons facility of a new muon beamline monitoring and novel digital data acquisition system for gamma... 相似文献