2-Amino-4-bromobutanoic Acid

(2S)- and (2R)-2-Amino-4-bromobutanoic acid were prepared starting from N-Boc-glutamic acid α tert-butyl ester. The double tert-butyl protection was necessary to prevent a partial racemisation during Barton’s radical decarboxylation used to transform the γ-carboxylic group into a bromide. This bromide reacted with different nitrogen, oxygen and sulphur nucleophiles to give nonnatural amino acids characterised by basic or heterocyclic side chains. The title compound was also used to prepare a conformationally constrained peptidomimetic.     

神光-Ⅱ装置三倍频实验中靶场单元技术的改进

 主要介绍为了满足神光-Ⅱ高功率激光装置三倍频光（351nm，3ω）的物理实验要求，靶场三倍频模拟光源和瞄准监视系统两个主要单元技术的改进，即三倍频模拟光源由基频光（1 053nm，3ω）通过腔外的KTP+BBO晶体倍频获得，再经八路分光系统和主激光耦合；瞄准监视系统由透射式光学系统改进为反射式光学系统，避免原系统存在较大的色差，提高瞄准精度。     

金属组学在急性心肌梗死早期诊断的应用

为探讨急性心肌梗死(AMI)患者血清中K⁺、Na⁺、Ca²⁺、Fe²⁺、Mg²⁺含量变化,并研究其与心肌梗死患者之间的关系。选取2022年5月至2023年2月收治的AMI患者37例,同时选取健康体检者35例作为对照组。依据入院时或体检时收集的抽血样本进行临床生化分析,比较两组间血清K⁺、Na⁺、Ca²⁺、Fe²⁺、Mg²⁺含量,采用判别方程、主成分分析法(PCA),判断分析哪种金属离子对于心肌梗死的诊断价值大。结果表明,AMI患者的血清中Ca²⁺和Fe²⁺含量低于健康对照组,差异具有统计学意义。基于血钙、铁水平两组具有显著性差异,以它们为基础进行判别分析,获得判别函数式。将血清中K⁺、Na⁺、Ca²⁺、Fe²⁺、Mg²⁺     

微区X射线荧光光谱成像法分析历史文物建筑混凝土材料成分及工艺

历史文物建筑修复与保护的首要步骤是对建材构件进行成分解析。但成型的混凝土构件成分复杂,传统技术难以直接分析。微区X射线荧光光谱具有速度快、无需前处理、可获得大面积高分辨的元素成像等优势,可用于此类混合物的原位分析。本文采用束斑为20μm的微区X射线荧光光谱仪,扫描成型混凝土构件以获得混合成分的元素分布图,结合基本参数定量法,对上海地区典型历史建筑混泥土构件进行元素定量。分析结果有效地解析了混凝土构建中骨料和凝胶材料的成分和含量,鉴别了涂层成分,解析了拌混工艺,鉴别了局部污染元素和致劣元素并分析致劣原因,为历史建筑修复材料选配、拌混工艺选择、除污及保护性预防劣化提供了科学的数据支撑。     

Speckle characteristics of simulated deep Fresnel region under shadowing effect

After the three-dimensional self-affine fractal random surface simulation, we use the optical scattering theory to calculate the deep Fresnel region speckle(DFRS) under consideration of the more strict shadowing effect. The evolution of DFRS with the scattering distance and the intensity probability distribution are studied. It is found that the morphology of the scatterer has an antisymmetric relationship with the intensity distribution of DFRS, and the effect of micro-lenses on the scattering surface causes the intensity probability distribution of DFRS to deviate from the Gaussian speckle in the high light intensity area.     

Andrographolide Inhibits Biofilm and Virulence in Listeria monocytogenes as a Quorum-Sensing Inhibitor

Listeria monocytogenes is a major foodborne pathogen that can cause listeriosis in humans and animals. Andrographolide is known as a natural antibiotic and exhibits good antibacterial activity. We aimed to investigate the effect of andrographolide on two quorum-sensing (QS) systems, LuxS/AI-2 and Agr/AIP of L. monocytogenes, as well as QS-controlled phenotypes in this study. Our results showed that neither luxS expression nor AI-2 production was affected by andrographolide. Nevertheless, andrographolide significantly reduced the expression levels of the agr genes and the activity of the agr promoter P₂. Results from the crystal violet staining method, confocal laser scanning microscopy (CLSM), and field emission scanning electron microscopy (FE-SEM) demonstrated that andrographolide remarkably inhibited the biofilm-forming ability of L. monocytogenes 10403S. The preformed biofilms were eradicated when exposed to andrographolide, and reduced surviving cells were also observed in treated biofilms. L. monocytogenes treated with andrographolide exhibited decreased ability to secrete LLO and adhere to and invade Caco-2 cells. Therefore, andrographolide is a potential QS inhibitor by targeting the Agr QS system to reduce biofilm formation and virulence of L. monocytogenes.     

Space-Air-Ground Integrated Mobile Crowdsensing for Partially Observable Data Collection by Multi-Scale Convolutional Graph Reinforcement Learning

Mobile crowdsensing (MCS) is attracting considerable attention in the past few years as a new paradigm for large-scale information sensing. Unmanned aerial vehicles (UAVs) have played a significant role in MCS tasks and served as crucial nodes in the newly-proposed space-air-ground integrated network (SAGIN). In this paper, we incorporate SAGIN into MCS task and present a Space-Air-Ground integrated Mobile CrowdSensing (SAG-MCS) problem. Based on multi-source observations from embedded sensors and satellites, an aerial UAV swarm is required to carry out energy-efficient data collection and recharging tasks. Up to date, few studies have explored such multi-task MCS problem with the cooperation of UAV swarm and satellites. To address this multi-agent problem, we propose a novel deep reinforcement learning (DRL) based method called Multi-Scale Soft Deep Recurrent Graph Network (ms-SDRGN). Our ms-SDRGN approach incorporates a multi-scale convolutional encoder to process multi-source raw observations for better feature exploitation. We also use a graph attention mechanism to model inter-UAV communications and aggregate extra neighboring information, and utilize a gated recurrent unit for long-term performance. In addition, a stochastic policy can be learned through a maximum-entropy method with an adjustable temperature parameter. Specifically, we design a heuristic reward function to encourage the agents to achieve global cooperation under partial observability. We train the model to convergence and conduct a series of case studies. Evaluation results show statistical significance and that ms-SDRGN outperforms three state-of-the-art DRL baselines in SAG-MCS. Compared with the best-performing baseline, ms-SDRGN improves 29.0% reward and 3.8% CFE score. We also investigate the scalability and robustness of ms-SDRGN towards DRL environments with diverse observation scales or demanding communication conditions.     

振动噪声等高图的电驱动桥噪声预测及优化*

在项目开发前期通过优化电驱动桥扭矩特性的设计,可以规避噪声大问题。根据电驱动桥台架在对应扭矩下的振动噪声特性,提出了一套稳定高效的测试流程和分析方法。首先设计了一套完整的试验流程,制定了精准的数据分析方法。然后绘制出能够全面反映电驱动桥振动噪声特性的等高图。最后利用电驱动桥台架的振动噪声等高图,准确评估电驱动桥加速工况下振动噪声风险,为主机厂和电驱动桥零部件企业提供电驱动桥扭矩特性设计前期指导。利用该方法成功识别到某电驱动桥匀速及加速工况下电机及齿轮的啸叫问题。通过优化电驱动桥扭矩特性设计,电机48阶噪声峰值降低了8.5dB(A),确认该方法准确可靠,具备推广应用价值。     

Synthesis of PNIPAAm-g-P4VP Microgel as Draw Agent in Forward Osmosis by RAFT Polymerization and Reverse Suspension Polymerization to Improve Water Flux

Microgels have unique and versatile properties allowing their use in forward osmosis areas as a draw agent. In this contribution, poly(4-vinylpyridine) (P4VP) was synthesized via RAFT polymerization and then grafted to a poly(N-Isopropylacrylamide) (PNIPAAm) crosslinking network by reverse suspension polymerization. P4VP was successfully obtained by the quasiliving polymerization with the result of nuclear magnetic resonance and gel permeation chromatography characterization. The particle size and particle size distribution of the PNIPAAm-g-P4VP microgels containing 0, 5, 10, 15 and 20 wt% P4VP were measured by means of a laser particle size analyzer. It was found that all the microgels were of micrometer scale and the particle size was increased with the P4VP load. Inter/intra-molecular-specific interactions, i.e., hydrogen bond interactions were then investigated by Fourier infrared spectroscopy. In addition, the water flux measurements showed that all the PNIPAAm-g-P4VP microgels can draw water more effectively than a blank PNIPAAm microgel. For the copolymer microgel incorporating 20 wt% P4VP, the water flux was measured to be 7.48 L∙m⁻²∙h⁻¹.