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991.
In this study, immunization with a vaccine consisting of multiple F(abt’)2 fragments of affinity-purified antitetanus toxoid antibodies covalently bound to a carrier protein successfully induced antitetanus
toxoid antibodies. Further studies showed that this vaccine preparation contained no biologically detectable tetanus antigen.
The induced antitetanus antibody (Ab1t’) titer was higher than the titer of antibodies binding control antigens. The immunizing
F(abt’)2 preparation did not elicit a secondary antitetanus response from mice primed with tetanus toxoid and, hence, appeared free
of tetanus epitopes. The specificity of Ab1t’ was established by absorption and inhibition with antigen. Immunization with
antitetanus F(abt’)2 (Ab1t’) fragments appears to have elicited naturally occurring autologous antitetanus toxoid antibody (Ab1t’) through an
idiotypic pathway. As predicted by network theory, anti-idiotype (Ab2) and antitetanus (Ab1t’) cycled reciprocally. Clonotypic
characterization of Ab1t’ using isoelectric focusing and affinity immunoblotting showed increases in Ab1t’ titer to be the
result of increased synthesis by limited subsets of antitetanus toxoid B-cell clones and not increased synthesis by multiple
clones, as is characteristic of antigen-driven Ab1 responses. Many Ab1 and Ab1t’ clonotypes had identical pIs, suggesting
that they either share V region genes or are the product of the same B-cell clones. These findings indicate that immunization
with polyclonal multivalent Ab1 preparations can trigger active synthesis of antibodies with the same specificity. The results
provide further evidence for naturally occurring idiotypic cascades that could be exploited for studies of catalytic antibodies. 相似文献
992.
Eberling J Braun P Kowalczyk D Schultz M Kunz H 《The Journal of organic chemistry》1996,61(8):2638-2646
The selective C-terminal deprotection of O-glycopeptide (methoxyethoxy)ethyl esters is achieved under mild conditions (pH 6.6, 37 degrees C) by enzymatic hydrolysis using papain or lipase M from Mucor javanicus to give building blocks useful for chain-extending glycopeptide synthesis. On the other hand, the selective removal of acetyl protecting groups from the saccharide portion of glycopeptides is accomplished by alternative enzymatic hydrolysis with lipase WG from wheat germ to furnish model substrates for enzymatic glycosyl transfer reactions in order to extend the carbohydrate side chain of these conjugates. 相似文献
993.
Hans Beat Bürgi Hermann Gehrer Peter Strickler Fritz Karl Winkler 《Helvetica chimica acta》1976,59(7):2558-2565
Crystals of the title compound are triclinic, a = 27.87 Å, b = 10.77 Å, c = 12.94 Å, α 73.1°, β 116.1°, γ 120.0°, space group P1 . The structure consists of octanuclear ions: Eight Cd(II) ions are found at the corners of a distorted cube, the center of the cube is occupied by an iodide, the twelve thioglycolate sulfur atoms bridge the twelve edges of the cube thereby forming a distorted icosahedron. Cadmium ions are either five or seven coordinate. The phase problem for this structure was solved using a combination of very high and very low E-values. 相似文献
994.
Dr. A. Woppmann 《Monatshefte für Chemie / Chemical Monthly》1974,105(2):419-426
The kinetics of the diazotization of o-, m-, p-chloroaniline in 0.005n- to 0.4n-methanolic HCl-solution at 25, 15, 0, ?10 ?20, and ?30°C was invertigated. It was found that the nitrosation reaction (the same as in1) $$C_6 H_4 ClNH_2 + NOCl \mathop \rightleftharpoons \limits^k C_6 H_4 ClNH_2 NO^ + + Cl^ - $$ is a proceeding advance-back-reaction. The decomposition of C6H4ClNH2NO+ by splitting off a proton is the rate determining step. The free activation enthalpies ΔG * for the nitrosation reaction, the activation entropies ΔS *, the activation enthalpies ΔH * and the activation energiesE a at the given temperatures are calculated. The experimentally found and the calculated velocities are given in Tables 1–6. The equilibrium constants of the o-, m-, p-chloroanilinium ions, and nitrosyl-chloride in methanol are indicated in Table 7, diagram 1. TheK M values (the ionic products of methanol, extrapolated at infinite dilution) together with theK A values of Table 7 give theK B values (p. 2) using the table10. The ΔG B values can be calculated using equation ΔG B = ?RTlnK B Fig 2 shows the linear dependance of the logarithmus of the ΔG * values from the logarithmus of theK B values. 相似文献
995.
The model of Onsager in which a polar molecule undergoes a reaction field due to the polarization of the molecular surroundings is used to evaluate by a S.C.F. calculation (CNDO/2 approximation) the modifications of a molecular structure in the liquid state.Application to water molecule and to three polar dimers for values of the dielectric constant varying between 3 and 78, shows that most of geometric parameters and dipoles moments vary of few per cent when the molecule is inserted in a liquid. In the liquid state dipole moments do not depend very much on the dielectric constant but energies and relative stabilities of isomers are strongly dependent on the medium.[/p] 相似文献
996.
Cytotoxicity and Antiproliferative Effect of Hypericin and Derivatives after Photosensitization 总被引:3,自引:1,他引:3
Ann L. Vandenbogaerde Els M. Delaey Annelies M. Vantieghem Bernard E. Himpens Wilfried J. Merlevede Peter A. de Witte 《Photochemistry and photobiology》1998,67(1):119-125
The toxicity on three human tumor cell lines (A431, HeLa and MCF7) of five phenanthroperylenequinones (hypericin and derivatives) and two perylenequinones (cercosporin and calphostin C) was investigated after photosensitization (4 J/cm2 ). Furthermore, the antiproliferative effect on HeLa cells was studied for the phenanthroperylenequinones. Hypericin, 2,5-dibromohypericin, 2,5,9,12-tetrabromohypericin and perylenequinones displayed a potent cytotoxic and antiproliferative effect in the nanomolar range. Hypericin dicarboxylic acid exhibited no photoactivity. In general, the antiproliferative activity correlated well with the photocytotoxicity. However, the nonphotocytotoxic compound hexamethylhypericin showed potent antiproliferative activity in the nanomolar range, probably exerting its action by protein kinase C inhibition. Without light irradiation, no cytotoxic and antiproliferative effect was observed for any photocytotoxic phenanthroperylenequinone compound. Furthermore, confocal laser microscopy revealed that the subcellular localization in A431 cells was similar for the photoactive compounds; the photosensitizers were mainly concentrated in the perinuclear region, probably corresponding with the Golgi apparatus and the endoplasmic reticulum. In addition, the accumulation of the photosensitizers in HeLa cells was investigated. All compounds except hypericin dicarboxylic acid were found to concentrate to a large extent in the cells. The compound 2,5,9,12-tetrabromohypericin seemed intrinsically more effective than hypericin since the intracellular concentration of the bromoderivative was a magnitude of order lower than that of hypericin although both compounds showed similar photobiological activity. 相似文献
997.
Simulations of diffusion-limited cluster-cluster aggregation (DLCA) with no restructuring, full restructuring, and partial restructuring have been performed. The scattering patterns produced from these aggregates have been simulated using the Rayleigh-Gans-Debye approximation. Pure DLCA aggregates produced a scattering pattern with the slope of the fractal region being about -1.8. In contrast, the slope of the fractal region of the scattering pattern for fully restructured aggregates was about -2.1, indicating an increase in fractal dimension. Partial restructuring at large length scales produced an upward turn in the scattering pattern at low qr(o), while at high qr(o) the fractal section of the pure DLCA aggregate was retained. This last result was expected and is consistent with the results and postulations of several other workers. This simulation shows that the type of scattering pattern often obtained from orthokinetic or sheared aggregation can be produced by restructuring of aggregates at large length scales. Copyright 2001 Academic Press. 相似文献
998.
Dr. Marita Fell 《Colloid and polymer science》1967,220(2):107-110
Zusammenfassung Aus einem durch Trypsinabbau von -Keratose erhaltenen Gemisch von Peptiden wurden 15 neutrale, 5 saure und 2 basische Peptide durch Kombination papierchromatographischer und papierelektrophoretischer Methoden getrennt und in sehr geringen Mengen isoliert. Es wurden neben der Aminosäurezusammensetzung 14N-terminale Aminosäuren sowie einige Teilsequenzen in den untersuchten Peptiden ermittelt.Die mittlere Kettenlänge der Peptide beträgt 5,8 Aminosäuren.Mit 1 Tabelle3. Mitt. über Untersuchungen an -Keratose. 2. Mitt.:M. Fell undE. Schnabel, Hoppe-Seyler's Z. physiol. Chem.333, 218 (1963). 相似文献
999.
The binding and availability of metals (Al, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Ni, Pb, Zn) in therapeutically applied peat (Grosses Gifhorner Moor, Sassenburg/North Germany) was characterized by means of a versatile extraction approach. Aqueous extracts of peat were obtained by a standardized batch equilibrium procedure using high-purity water (pH 4.5 and 5.0), 0.01 mol l(-1) calcium chloride solution, 0.01 mol l(-1) ethylenediaminetetraacetic acid (EDTA) and 0.01 mol l(-1) diethylenetriamine pentaacetic acid (DTPA) solution as metal extractants. In addition, the availability of peat-bound metal species was kinetically studied by collecting aliquots of extracts after different periods of extraction time (5, 10, 15, 30, 60 and 120 min). Metal determinations were performed by atomic spectrometry methods (AAS, ICP-OES) and dissolved organic matter (DOM) was characterized by UV/Vis measurements at 254 and 436 nm, respectively. Of the extractants studied Ca, Mg and Mn were the most available metals, in contrast to peat-bound Fe and Al. The relative standard deviation s(r) of the developed extraction procedures was mostly in the range of 4 to 20%, depending on the metal and its concentration in peat. A pH increase favored the extraction of metals and DOM from peat revealing complex extraction kinetics. Moreover, a competitive exchange between peat-bound metal species and added Cu(II) ions showed that >100 mg of Cu(II) per 50 g wet peat was necessary to exchange the maximum of bound metals (e.g. 21.8% of Al, 3.9% of Fe, 79.0% of Mn, 81.9% of Sr, related to their total content). 相似文献
1000.
de la Fuente JM Eaton P Barrientos AG Menéndez M Penadés S 《Journal of the American Chemical Society》2005,127(17):6192-6197
Thermodynamic evidence for the selective Ca(2+)-mediated self-aggregation via carbohydrate-carbohydrate interactions of gold glyconanoparticles functionalized with the disaccharides lactose (lacto-Au) and maltose (malto-Au), or the biologically relevant trisaccharide Lewis X (Le(X)-Au), was obtained by isothermal titration calorimetry. The aggregation process was also directly visualized by atomic force microscopy. It was shown in the case of the trisaccharide Lewis X that the Ca(2+)-mediated aggregation is a slow process that takes place with a decrease in enthalpy of 160 +/- 30 kcal mol(-)(1), while the heat evolved in the case of lactose and maltose glyconanoparticles was very low and thermal equilibrium was quickly achieved. Measurements in the presence of Mg(2+) and Na(+) cations confirm the selectivity for Ca(2+) of Le(X)-Au glyconanoparticles. The relevance of this result to cell-cell adhesion process mediated by carbohydrate-carbohydrate interactions is discussed. 相似文献