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Myoglobin CNBr peptides, constituting the commercially available molecular weight calibration kits for sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were analyzed by microsequencing after electroblotting on polyvinylidene difluoride (Immobilon) membranes. An obvious disagreement was found between peptide identification and the data provided by the manufacturers. We observed 6 peptides from Mr 2500 to 17,000 corresponding, in increasing size order, to the 3 peptides resulting from the total CNBr digestion, to 2 incompletely cleaved peptides and to the intact myoglobin. Using a corrected calibration curve, a linear relationship was established from Mr 6000 to 43,000 and a second one for shorter peptides. This method of electrophoresis and electroblotting, easily adapted for peptides, is a powerful tool for peptide identification correlated with size determination. It is especially useful for CNBr-cleaved peptides. 相似文献
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Haines TJ Bionta RM Blewitt G Bratton CB Casper D Claus R Cortez BG Errede S Foster GW Gajewski W Ganezer KS Goldhaber M Jones TW Kielczewska D Kropp WR Learned JG Lehmann E LoSecco JM Matthews J Park HS Price LR Reines F Schultz J Seidel S Shumard E Sinclair D Sobel HW Stone JL Sulak L Svoboda R van der Velde JC Wuest C 《Physical review letters》1986,57(16):1986-1989
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Observation of a neutrino burst in coincidence with supernova 1987A in the Large Magellanic Cloud 总被引:1,自引:0,他引:1
Bionta RM Blewitt G Bratton CB Casper D Ciocio A Claus R Cortez B Crouch M Dye ST Errede S Foster GW Gajewski W Ganezer KS Goldhaber M Haines TJ Jones TW Kielczewska D Kropp WR Learned JG LoSecco JM Matthews J Miller R Mudan MS Park HS Price LR Reines F Schultz J Seidel S Shumard E Sinclair D Sobel HW Stone JL Sulak LR Svoboda R Thornton G van der Velde JC Wuest C 《Physical review letters》1987,58(14):1494-1496
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An alternating direction scheme on a nonuniform mesh for reaction-diffusion parabolic problems 总被引:1,自引:0,他引:1
Clavero C; Jorge JC; Lisbona F; Shishkin={paragraph} GI 《IMA Journal of Numerical Analysis》2000,20(2):263-280
In this paper we develop a numerical method for two-dimensionaltime-dependent reaction-diffusion problems. This method, whichcan immediately be generalized to higher dimensions, is shownto be uniformly convergent with respect to the diffusion problems.This method, which can immediately be generalized to higherdimensions, is shown to be uniformly convergent with respectto the diffusion parameter. 相似文献
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OK Yoon WG Hwang JC Choe MS Kim 《Rapid communications in mass spectrometry : RCM》1999,13(14):1515-1521
A technique to investigate photodissociation kinetics on a nanosecond time scale has been devised for molecular ions generated by multiphoton ionization (MPI) using mass-analyzed ion kinetic energy spectrometry. The branching ratio or rate constant has been determined for the photodissociation of the n-butylbenzene, bromobenzene, iodobenzene, and aniline molecular ions generated by MPI at 266 nm. The ion internal energies have been estimated by comparing the measured kinetic data with the previous energy dependence data. The analysis has shown that only those molecular ions generated by two-photon ionization contribute to the photodissociation signals. Around half of the available energy has been found to remain as molecular ion internal energy in the two-photon ionization process. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
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Jason T Miller John H Bartley Hereward JC Wimborne Aisha L Walker David C Hess William D Hill James E Carroll 《BMC neuroscience》2005,6(1):63
Background
Stromal cell-derived factor 1 (SDF-1 or CXCL12) is chemotaxic for CXCR4 expressing bone marrow-derived cells. It functions in brain embryonic development and in response to ischemic injury in helping guide neuroblast migration and vasculogenesis. In experimental adult stroke models SDF-1 is expressed perivascularly in the injured region up to 30 days after the injury, suggesting it could be a therapeutic target for tissue repair strategies. We hypothesized that SDF-1 would be expressed in similar temporal and spatial patterns following hypoxic-ischemic (HI) injury in neonatal brain. 相似文献20.
α-Haloketones and -dibromides are converted to the corresponding ketones and alkenes respectively with nickel boride generated from sodium borohydride and nickel chloride. 相似文献