Ankyrin repeat and SOCS box containing protein 4 (Asb-4) colocalizes with insulin receptor substrate 4 (IRS4) in the hypothalamic neurons and mediates IRS4 degradation

Background

Cigarette smoking enhances the risk of stroke. However, the underlying molecular mechanisms are largely unknown. The present study established an in vivo rat secondhand cigarette smoking (SHS) model and examined the hypothesis that SHS upregulates endothelin receptors with increased cerebrovascular contraction via the Raf/extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinases (MAPK) pathway.

Results

Rats were exposed to SHS for up to 8 weeks. The cerebral artery vasoconstriction was recorded by a sensitive myograph. The mRNA and protein expressions for endothelin receptors in cerebral arteries were studied by real-time PCR and Western blot. Compared to fresh air exposed rats, cerebral arteries from SHS rats exhibited stronger contractile responses (P < 0.05) mediated by endothelin type A (ET_A) receptors. The expressions of mRNA and protein for ET_A receptors in the cerebral arteries from SHS rats were higher (P < 0.05) than that in control. SHS did not affect endothelin type B (ET_B) receptor-mediated contractions, mRNA or protein levels. The results suggest that SHS upregulates ET_A, but not ET_B receptors in vivo. After SHS exposure, the mRNA levels of Raf-1 and ERK1/2, the protein expression of phosphorylated (p)-Raf-1 and p-ERK1/2 were increased (P < 0.05). Raf-1 inhibitor, GW5074 suppressed the enhanced ET_A receptor-mediated contraction, mRNA and protein levels induced by SHS. In addition, GW5074 inhibited the SHS-caused increased mRNA and phosphorylated protein levels of Raf-1 and ERK1/2, suggesting that SHS induces activation of the Raf/ERK/MAPK pathway.

Conclusions

SHS upregulates cerebrovascular ET_A receptors via the Raf/ERK/MAPK pathway, which provides novel understanding of mechanisms involved in SHS-associated stroke.     

A simultaneous in- and out-of-plane displacement measurement method

A method is presented that allows real-time three-dimensional displacement maps to be obtained for deformable objects using a single color camera and a color fringe projector. The acquired images have speckle and fringe information embedded in them, which are separated using the R, G, and B color signals from a color CCD camera to permit the independent use of a two-dimensional digital image correlation to obtain the x- and y-direction displacements and fringe projection to obtain the displacement in the z direction.     

Noise-assisted multibit storage device

In this Letter we extend our investigations on noise-assisted storage devices through the experimental study of a loop composed of a single Schmitt trigger and an element that introduces a finite delay. We show that such a system allows the storage of several bits and does so more efficiently for an intermediate range of noise intensities. Finally, we study the probability of erroneous information retrieval as a function of elapsed time and show a way for predicting device performance independently of the number of stored bits.     

A rational decision making model for experimental mechanics

Experimental Techniques - A rational decision-making model with conjunctive and compensatory rules has been proposed and demonstrated for use in experimental mechanics. The model contains a logical...     

The mechanical optical fiber splice for the 1990s

Splicing of optical fibers will be a major determining factor in the technical and economic success of fiber networks built in the 1990s. Needs, requirements, and specifications are now being proposed for the splicing systems to be used in these future networks. Two basic objectives for the splicing system of the 1990s can be defined from all the study and analysis that has been done thus far. Simply stated, the most widely accepted splicing system for the future will be the one that provides the highest performance at the lowest installed cost.

A new splicing system has been developed that addresses and meets many of the requirements that have been proposed. This system provides high splice performance and at the same time is very easy and fast to use. It also has low-cost assembly tooling and has been exhaustively tested to ensure conformance with proposed performance specifications.     

Evidence for the decay D0-->K(-)pi(+)pi(-)e(+)nu(e)

Using a 281 pb{-1} data sample collected at the psi(3770) with the CLEO-c detector, we present the first absolute branching fraction measurement of the decay D0-->K(-)pi(+)pi(-)e(+)nu(e) at a statistical significance of about 4.0 standard deviations. We find 10 candidates consistent with the decay D0-->K(-)pi(+)pi(-)e(+)nu(e). The probability that a background fluctuation accounts for this signal is less than 4.1 x 10{-5}. We find B(D0-->K(-)pi(+)pi(-)e(+)nu(e)) = [2.8{-1.1}{+1.4}(stat)+/-0.3(syst)]x10{-4}. By restricting the invariant mass of the hadronic system to be consistent with K1(1270), we obtain the product of branching fractions B(D{0}-->K{1}{-}(1270)e{+}nu{e})xB(K1-(1270)-->K{-}pi{+}pi{-})=[2.5{-1.0}{+1.3}(stat)+/-0.2(syst)]x10{-4}. Using B(K1-(1270)-->K{-}pi{+}pi{-})=(33+/-3)%, we obtain B(D{0}-->K{1}{-}(1270)e{+}nu{e})=[7.6{-3.0}{+4.1}(stat)+/-0.6(syst)+/-0.7]x10{-4}. The last error accounts for the uncertainties in the measured K1-(1270)-->K{-}pi{+}pi{-} branching fractions.