Catalytic conversion of lactic acid into propylene glycol over various metals supported on silica

Catalytic hydrogenation of lactic acid to propylene glycol was performed over various metals (Ag, Co, Cu, Ni, Pt, and Ru) supported on silica prepared by an incipient wetness impregnation method. The loading amount of each metal was 5 wt%. Crystallinity of the synthesized catalysts was investigated by X-ray diffraction (XRD), and the BET method was utilized to examine the surface area. Pore volume and pore size of catalysts were determined using BJH analysis of the N₂ adsorption isotherm. Particle sizes of various metals were determined from transmission electron microscopy (TEM) images. The catalytic activity was found to be strongly dependent on the supported metal. Among catalysts tested, Ru/SiO₂ showed the highest propylene glycol yield. The yield of propylene glycol increased with pressure, and the highest yield was achieved at 130 °C.     

Photoinduced superoxide radical anion and singlet oxygen generation in the presence of novel selenadiazoloquinolones (an EPR Study)

Novel 7‐substituted 6‐oxo‐6,9‐dihydro[1,2,5]selenadiazolo[3,4‐h]quinoline ( SeQ(1–6) ) and 8‐substituted 9‐oxo‐6,9‐dihydro[1,2,5]selenadiazolo[3,4‐f ]quinoline derivatives ( SeQN(1–5) ) with R₇, R₈ = H, COOC₂H₅, COOCH₃, COOH, COCH₃ or CN were synthesized and their spectral characteristics were obtained by UV/Vis spectroscopy. Ultraviolet A photoexcitation of the selenadiazoloquinolones in dimethylsulfoxide or acetonitrile resulted in the formation of paramagnetic species coupled with molecular oxygen activation generating the superoxide radical anion or singlet oxygen, evidenced by electron paramagnetic resonance spectroscopy. The cytotoxic/photocytotoxic impact of selenadiazoloquinolones on murine and human cancer cell lines was demonstrated using the derivative SeQ5 (with R₇ = COCH₃).     

Lipophilicity of Tetraarylporphyrins. Part 1. Tetra-(Hydroxyphenyl)Porphyrins with Long Alkyl Chain in the Molecule

JPC – Journal of Planar Chromatography – Modern TLC - In the phototherapeutic use of photosensitizers, lipophilicity is an important parameter that allows predicting their biological...     

Quantification of human growth hormone by amino acid composition analysis using isotope dilution liquid-chromatography tandem mass spectrometry

We describe an accurate method for protein quantification based on conventional acid hydrolysis and an isotope dilution-HPLC-mass spectrometry (ID-HPLC-MS) method. Sample purity was confirmed using capillary zone electrophoresis, HPLC and MS. The analyte protein, human growth hormone (hGH), was effectively hydrolyzed by incubation with 8 M hydrochloric acid at 130 °C for 48 h, where at least 1 μM of hGH was treated to avoid possible degradation of released amino acids during hydrolysis. Using a reversed-phase column, the analytes (isoleucine, phenylalanine, proline and valine) were separated within 5 min using an isocratic eluent comprising 10% acetonitrile containing 0.1% trifluoroacetic acid. The detection limit (signal to noise ratio of 3) of amino acids was 5.5-6.2 fmol per injection. The quantification precision (RSD) of amino acids for intra- and inter-day assays was less than 0.98% and 0.39%, respectively. Comparison with other biochemical and instrumental methods revealed substantially higher accuracy and reproducibility of the ID-HPLC-MS/MS method as expected. The optimized hydrolysis and analytical conditions in our study were suitable for accurate quantification of hGH.     

Hydrophoretic high-throughput selection of platelets in physiological shear-stress range

A gentle, but fast means for low-stress, high-throughput platelet purification is of significant clinical and biotechnological utility. Current implementations to sort platelets, however, require an external physical field, specialized buffer, or the harsh separation condition of high shear stress that tends to cause platelet stimulation. Here we report the use of hydrophoretic size separation in a wider channel and its parallelization to augment its throughput capability, maintaining physiological shear-stress range. We demonstrate a parallelized device comprising 10 stacks of the wide-channel hydrophoresis device, yielding a throughput of 2.9 million cells s(-1) and a platelet purity of 76.8%. The use of the wide channel for hydrophoresis also facilitates clogging-free separation by sorting blood clots and plaques. The wide-channel hydrophoresis offers the potential for gentle, fast, clogging-free sorting of rare blood cells with extreme throughput capabilities.     

Analysis of space–time discontinuous Galerkin method for nonlinear convection–diffusion problems

The paper presents the theory of the discontinuous Galerkin finite element method for the space–time discretization of a nonstationary convection–diffusion initial-boundary value problem with nonlinear convection and linear diffusion. The problem is not singularly perturbed with dominating convection. The discontinuous Galerkin method is applied separately in space and time using, in general, different space grids on different time levels and different polynomial degrees p and q in space and time dicretization. In the space discretization the nonsymmetric, symmetric and incomplete interior and boundary penalty (NIPG, SIPG, IIPG) approximation of diffusion terms is used. The paper is concerned with the proof of error estimates in “L ²(L ²)”- and “DG”-norm formed by the “L ²(H ¹)”-seminorm and penalty terms. A special technique based on the use of the Gauss–Radau interpolation and numerical integration has been used for the derivation of an abstract error estimate. In the “DG”-norm the error estimates are optimal with respect to the size of the space grid. They are optimal with respect to the time step, if the Dirichlet boundary condition has behaviour in time as a polynomial of degree ≤ q.     

Solutions to the polynomial Dirac equations on unbounded domains in Clifford analysis

In this paper we study polynomial Dirac equation p(??)f = 0 including (?? ? λ)f = 0 with complex parameter λ and ??^kf = 0(k?1) as special cases over unbounded subdomains of ?^{n + 1}. Using the Clifford calculus, we obtain the integral representation theorems for solutions to the equations satisfying certain decay conditions at infinity over unbounded subdomains of ?^{n + 1}. Copyright © 2010 John Wiley & Sons, Ltd.     

On 2-Connected Spanning Subgraphs with Bounded Degree in K 1,r -Free Graphs

For any integer r > 1, an r-trestle of a graph G is a 2-connected spanning subgraph F with maximum degree Δ(F) ≤ r. A graph G is called K _1,r -free if G has no K _1,r as an induced subgraph. Inspired by the work of Ryjáček and Tkáč, we show that every 2-connected K _1,r -free graph has an r-trestle. The paper concludes with a corollary of this result for the existence of k-walks.