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621.
Teulade-Fichou MP Carrasco C Guittat L Bailly C Alberti P Mergny JL David A Lehn JM Wilson WD 《Journal of the American Chemical Society》2003,125(16):4732-4740
The interaction of G-quadruplex DNA with the macrocyclic compound BOQ1, which possesses two dibenzophenanthroline (quinacridine) subunits, has been investigated by a variety of methods. The oligonucleotide 5'-A(GGGT(2)A)(3)G(3), which mimics the human telomeric repeat sequence and forms an intramolecular quadruplex, was used as one model system. Equilibrium binding constants measured by biosensor surface plasmon resonance (SPR) methods indicate a high affinity of the macrocycle for the quadruplex conformation (K > 1 x 10(7) M(-)(1)) with two equivalent binding sites. The affinity of BOQ1 for DNA duplexes is at least 1 order of magnitude lower. In addition, the macrocycle is more selective than the monomeric control compound (MOQ2), which is not able to discriminate between the two DNA structures (K(duplex) approximately K(quadruplex) approximately 10(6) M(-)(1)). Strong binding of BOQ1 to G4 DNA sequences was confirmed by fluorometric titrations with a tetraplex-forming oligonucleotide. Competition dialysis experiments with a panel of different DNA structures, from single strands to quadruplexes, clearly established the quadruplex binding specificity of BOQ1. Fluorescence resonance energy transfer (FRET) T(m) experiments with a doubly labeled oligonucleotide also revealed a strong stabilization of the G4 conformation in the presence of BOQ1 (DeltaT(m) = +28 degrees C). This DeltaT(m) value is one of the highest values measured for a G-quadruplex ligand and is significantly higher than observed for the monomer control compounds (DeltaT(m) = +10-12 degrees C). Gel mobility shift assays indicated that the macrocycle efficiently induces the formation of G-tetraplexes. Strong inhibition of telomerase was observed in the submicromolar range (IC(50) = 0.13 microM). These results indicate that macrocycles represent an exciting new development opportunity for targeting DNA quadruplexes. 相似文献
622.
Automated headspace solid-phase dynamic extraction to analyse the volatile fraction of food matrices 总被引:2,自引:0,他引:2
Bicchi C Cordero C Liberto E Rubiolo P Sgorbini B 《Journal of chromatography. A》2004,1024(1-2):217-226
High concentration capacity headspace techniques (headspace solid-phase microextraction (HS-SPME) and headspace sorptive extraction (HSSE)) are a bridge between static and dynamic headspace, since they give high concentration factors as does dynamic headspace (D-HS), and are as easy to apply and as reproducible as static headspace (S-HS). In 2000, Chromtech (Idstein, Germany) introduced an inside-needle technique for vapour and liquid sampling, solid-phase dynamic extraction (SPDE), also known as "the magic needle". In SPDE, analytes are concentrated on a 50 microm film of polydimethylsiloxane (PDMS) and activated carbon (10%) coated onto the inside wall of the stainless steel needle (5 cm) of a 2.5 ml gas tight syringe. When SPDE is used for headspace sampling (HS-SPDE), a fixed volume of the headspace of the sample under investigation is sucked up an appropriate number of times with the gas tight syringe and an analyte amount suitable for a reliable GC or GC-MS analysis accumulates in the polymer coating the needle wall. This article describes the preliminary results of both a study on the optimisation of sampling parameters conditioning HS-SPDE recovery, through the analysis of a standard mixture of highly volatile compounds (beta-pinene, isoamyl acetate and linalool) and of the HS-SPDE-GC-MS analyses of aromatic plants and food matrices. This study shows that HS-SPDE is a successful technique for HS-sampling with high concentration capability, good repeatability and intermediate precision, also when it is compared to HS-SPME. 相似文献
623.
Iwao Ojima Robert J. Donovan Patrizia Ingallina Núria Clos William R. Shay Masakatsu Eguchi Qingping Zeng Anna Korda 《Journal of Cluster Science》1992,3(4):423-438
The reactions of hydrosilane and/or alkyne as well as isonitriles with rhodium and rhodium cobalt mixed metal carbonyl clusters, e.g., Rh4(CO)12 and Co2Rh2(CO)12, are studied. Novel mixed metal complexes, e.g., CoRh(CO)5 (HCCBu
n
), (R3Si)2Rh(CO)
n
Co(CO)4, Rh(R–NC)4Co(CO)4, Co2Rh2(CO)10(HCCR), and Co2Rh2(CO)9(HCCBu
n
), are synthesized and identified. The catalytic activities of these rhodium and rhodium-cobalt mixed metal complexes are examined in hydrosilyation, silylformylation, and novel silylcarbocyclization reactions. Possible mechanisms for these reactions are proposed and discussed. 相似文献
624.
Bifurcation of fixed points from a manifold of trivial fixed points in the infinite-dimensional case
Massimo Furi Mario Martelli Maria Patrizia Pera 《Journal of Fixed Point Theory and Applications》2007,2(2):293-312
We obtain a necessary as well as a sufficient condition for the existence of bifurcation points of a coincidence equation,
and, in particular, of a parametrized fixed point problem. In both cases the trivial solutions are assumed to form a finite-dimensional
submanifold of a Banach manifold. An application is given to a delay differential equation on a manifold: we detect periodic
solutions that rotate close to an equilibrium point.
To Albrecht Dold and Edward Fadell, superb mathematicians and first rate friends 相似文献
625.
626.
Enzo Santaniello Patrizia Ferraboschi Shahrzad Reza-Elahi 《Monatshefte für Chemie / Chemical Monthly》2000,11(2):617-622
Lipase from Pseudomonas cepacia (PCL) catalyzes the regio- and stereoselective acylation of primary and secondary hydroxy groups in steroid side chains under irreversible transesterification conditions with vinyl acetate in organic solvents. 相似文献
627.
Paolo Antonini Dimitri Mugnai Patrizia Pucci 《Journal de Mathématiques Pures et Appliquées》2007,87(6):582-600
We prove maximum and comparison principles for weak distributional solutions of quasilinear, possibly singular or degenerate, elliptic differential inequalities in divergence form on complete Riemannian manifolds. A new definition of ellipticity for nonlinear operators on Riemannian manifolds is introduced, covering the standard important examples. As an application, uniqueness results for some related boundary value problems are presented. 相似文献
628.
Patrizia Pucci Raffaella Servadei 《Annales de l'Institut Henri Poincaré (C) Analyse Non Linéaire》2008
By the Mountain Pass Theorem and the constrained minimization method existence of positive or compactly supported radial ground states for quasilinear singular elliptic equations with weights are established. The paper also includes the discussion of regularity and the validity of useful qualitative properties of the solutions, which seems of independent interest. Finally, a Pohozaev type identity is produced to deduce some non-existence results. 相似文献
629.
Pierluigi Benevieri Massimo Furi Maria Patrizia Pera 《Journal of Differential Equations》2007,233(2):404-416
We prove a global bifurcation result for T-periodic solutions of the T-periodic delay differential equation x′(t)=λf(t,x(t),x(t−1)) depending on a real parameter λ?0. The approach is based on the fixed point index theory for maps on ANRs. 相似文献
630.
An application in cultural heritage is introduced. Wavelet decomposition and Neural Networks like virtual sensors are jointly used to simulate physical and chemical measurements in specific locations of a monument. Virtual sensors, suitably trained and tested, can substitute real sensors in monitoring the monument surface quality, while the real ones should be installed for a long time and at high costs. The application of the wavelet decomposition to the environmental data series allows getting the treatment of underlying temporal structure at low frequencies. Consequently a separate training of suitable Elman Neural Networks for high/low components can be performed, thus improving the networks convergence in learning time and measurement accuracy in working time. 相似文献