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41.
Pandurangan Muthuraman Sambandam Ravikumar Veerappan Muthuviveganandavel Jongpil Kim 《Applied biochemistry and biotechnology》2014,172(6):3153-3162
The present study was carried out to understand the effect of cortisol on calpain system in the C2C12 and 3T3-L1 adipocyte cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4 μm porous membrane to separate C2C12 and 3T3-L1 preadipocyte cells. Each cell type was grown independently on the transwell plates. Following cell differentiation, inserts containing 3T3-L1 cells were transferred to C2C12 plates. Ten microgram per milliliter of cortisol was added to the medium. Following treatment for 3 days, the cells in the lower well were harvested for analysis. Calpains such as μ-calpain, m-calpain, and calpastatin were selected for the analysis. RT-PCR results indicated the significant increase in the mRNA expression of μ-calpain, m-calpain, and calpastatin. In addition, the confocal microscopical investigation indicated the cortisol treatment increases calpain expression in the C2C12 and 3T3-L1 cells. Taking all these together, cortisol treatment with co-culture system shows most reliable status of calpains expression in the cells, which is quite distinct from one-dimensional monocultured cells. 相似文献
42.
Two acrylate monomers – 4‐(2′‐methoxycinnamoyl)phenyl acrylate, and 4‐(2′,5′‐dimethoxycinnamoyl)phenyl acrylate – comprising photocrosslinkable pendant chalcone moiety and a free radical polymerizable group were synthesized. The monomers were polymerized in the presence of ethyl methyl ketone at 70°C using benzoyl peroxide as the initiator. The polymers were characterized by UV, FT‐IR, 1H‐NMR, and 13C‐NMR spectra. The weight and number average molecular weights of the polymers were determined by gel permeation chromatography. The thermal stability of the polymers was studied by TGA under a nitrogen atmosphere. Glass transition temperatures of the polymers were studied by differential scanning calorimetry. The photoreactivity of the polymers was investigated for potential applications as photoresists in solution using various solvents. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
43.
Cobalt-doped cadmium sulphide (CdS) nanocrystals (NCs) were synthesized with three different cobalt concentrations by aqueous
chemical coprecipitation method. Dopant incorporation was recognised using X-ray diffraction (XRD) analysis with a shift in
the diffraction peaks and compression in the lattice. The size and crystallinity of the cobalt-doped CdS NCs were studied
by high resolution transmission electron microscopy (HRTEM). The blue shift in the spectra and the band gap value of Co-doped
CdS NCs was estimated using reflectance UV spectrophotometer. Variation in the luminescence properties was studied by fluorescence
spectroscopy. The change in the optical properties supports the Co incorporation in the CdS NCs. The BET measurement revealed
that the powders had a relatively high specific surface area of 131.30, 106.93 and 102.04 m2/g for 2, 4 and 6% Co:CdS, respectively. Room temperature magnetisation was studied using vibrating sample magnetometer for
both 4 and 6% cobalt-doped CdS NCs, which revealed a weak ferromagnetic signal and strong ferromagnetic hysteresis respectively. 相似文献
44.
Thiruneelakandan Chockalingam Manohar Muthuraman Rajkumar Seenivasan Rajagopal 《Transition Metal Chemistry》2006,31(4):549-555
The electron transfer reaction of Cr(VI) with organic sulfides is facilitated by added pyridine bases 2,2′-bipyridine, 1,10-phenanthroline,
picolinic acid and pyridine 2,6-dicarboxylic acid. The added base forms a Cr(VI)-base adduct carrying positive charge which
acts as a more reactive oxidant compared to Cr(VI). UV–Visible spectrophotometric study provides evidence for the formation
of Cr(V) as the intermediate. The reactions in the absence and presence of pyridine bases follow the reactivity–selectivity
principle. 相似文献
45.
The mesoporous Si-MCM-41 was synthesized by hydrothermal method and various wt.% (20 and 30 wt.%) of HPW were loaded on Si-MCM-41 by wet impregnation method. The synthesized Si-MCM-41 and HPW-loaded catalysts were characterized by XRD, BET surface area, FT-IR, TEM and TGA–DTG techniques. The catalytic activity of the catalyst was tested over the condensation reaction of aniline with various aromatic aldehydes at refluxing temperature under liquid-phase condition, which yields highly commercial product namely diamino triphenyl methanes (DATPMs). The effects of various parameters like catalyst, mole ratio, solvents and substituent effect on the formation of DATPMs were optimized. The catalytic activity of the catalysts showed the following order: H3PW12O40·nH2O > H3PMo12O40·nH2O > H4SiW12O40·nH2O > 20 wt.% HPW/MCM-41 > 30 wt.% HPW/MCM-41 > HM (12) > Hβ (8) > HY (4) > HZSM-5 (15) > Al-MCM-41 (25). The results showed that mole ratio of 4:1 (aniline:aldehyde) gave higher yield than the other mole ratios. Acetonitrile and ethyl acetate shows better activity especially in the supported materials than toluene was used as a solvent. The product thus obtained was analyzed by 1H NMR, FT-IR techniques. 相似文献
46.
Ghedeir M. Alshammari Abu ElGasim A. Yagoub Pandurangan Subash-Babu Amro B. Hassan Doha M. Al-Nouri Mohammed A. Mohammed Mohammed A. Yahya Rasha Elsayim 《Molecules (Basel, Switzerland)》2021,26(23)
The present study reports a cost-effective, environmentally friendly method to increase the bioavailability and bio-efficacy of B. rufescens stem bark extract in the biological system via functional modification as B. rufescens stem bark nanoparticles (BR-TO2-NPs). The biosynthesis of BR- -NPs was confirmed by UV-visible (UV-vis) and Fourier-transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction analyses. The shifts in FT-IR stretching vibrations of carboxylic and nitro groups (1615 cm−1), the O–H of phenolics or carboxylic acids (3405 cm−1), alkanes, and alkyne groups (2925 and 2224 cm−1) of the plant extract and lattice (455) indicated successful biosynthesis of BR- -NPs. Compared with the stem bark extract, 40 ng/dL dose of BR- -NPs led to a reduction in adipogenesis and an increase in mitochondrial biogenesis-related gene expressions, adiponectin-R1, PPARγC1α, UCP-1, and PRDM16, in maturing-adipocytes. This confirmed the intracellular uptake, bioavailability, and bio-efficiency of BR-TiO2-NPs. The lipid-lowering capacity of BR-TiO2-NPs effectively inhibited the metabolic inflammation-related gene markers, IL-6, TNF-α, LTB4-R, and Nf-κb. Further, BR-TiO2-NPs stimulating mitochondrial thermogenesis capacity was proven by the significantly enhanced CREB-1 and AMPK protein levels in adipocytes. In conclusion, BR-TiO2-NPs effectively inhibited lipid accumulation and proinflammatory adipokine levels in maturing adipocytes; it may help to overcome obesity-associated comorbidities. 相似文献
47.
Rhodium(II) catalyzed carbenoid reactions of 3-diazopiperidin-2-ones were carried out with indoles and pyrroles to afford the respective (3-indol-3-yl)- and (3-pyrrol-2-yl)piperidones with regioselectivity. Interestingly, the reaction of bis-diazoimide in the presence of Rh2(OAc)4 catalyst furnished diazacyclopenta[a]phenalenone in a tandem manner. 相似文献
48.
Triphenylphosphine-mediated reactions of diazoimides in water were carried out under mild conditions to afford several triazine derivatives in high yields. We have demonstrated an environment friendly methodology to synthesize triazine derivatives. 相似文献
49.
Narendran Rajendran Shankar Subramaniam Lowrence Rene Christena Meenakshi Sundaram Muthuraman Nagarajan Sai Subramanian Brindha Pemiah 《Natural product research》2016,30(17):2002-2006
Scutellaria oblonga Benth., a hitherto phytochemically unexplored Indian medicinal folklore plant was extracted with acetone and subjected to chromatography to yield nine flavonoids, for the first time from this plant. Antimicrobial assays were performed against 11 foodborne pathogens, and three molecules (Techtochrysin, Negletein and Quercitin-3-glucoside) depicted significant activity. These molecules were assessed for their rate of antibacterial action using time–kill curves which depicted complete inhibition of most of the bacteria within 12–16 h. The significant biofilm-reducing capability exhibited by these three molecules formed a significant finding of the current study. In most of the experiments, a 90–95% reduction in biofilms was observed. Thus, flavonoids as natural molecules from S. oblonga could be further researched to be used as potent antimicrobial and antibiofilm agents. 相似文献
50.
Khian Giap Lim Rajavel Varatharajan Arunachalam Muthuraman 《Molecules (Basel, Switzerland)》2022,27(13)
This study investigated the ameliorative effects of beta-carotene (BC) on diabetes-associated vascular dementia and its action against biomolecule oxidation. The diabetic vascular dementia (VaD) was induced by administration of nicotinamide (NA; 50 mg/kg; i.p.) and streptozotocin (STZ; 50 mg/kg; i.p.). The test compound, BC (50 and 100 mg/kg; p.o.), and the reference compound, donepezil (DP) (1 mg/kg; p.o.), were administered for 15 consecutive days. Changes in learning and memory were assessed by escape latency time (ELT) and times spent in target quadrant (TSTQ) in the Morris water maze (MWM) test. The changes in neurotransmitter, i.e., acetylcholinesterase (AChE) and oxidative stress markers, i.e., thiobarbituric acid reactive substance (TBARS) and reduced glutathione (GSH), were estimated in hippocampal tissue of the rat brain. The administration of STZ caused significant deterioration of cognitive function (decreased ELT and raised the TSTQ) as compared to the normal group. Treatment with BC and DP diminished the increased AChE activity, TBARS level and decreased GSH level caused by STZ. Thus, BC ameliorates the diabetic vascular complications in VaD due to its potential anticholinergic, antioxidative and free radical scavenging actions. 相似文献