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121.
Efstathios A. Sinouris Spyros S. Skandalis Virginia Kilia Achilleas D. Theocharis Dimitrios A. Theocharis Panagiota Ravazoula Demitrios H. Vynios Nickoletta Papageorgakopoulou 《Biomedical chromatography : BMC》2009,23(5):455-463
Proteoglycans (PGs) are widely expressed in all areas of the brain. In this study, the keratan sulfate‐containing PGs (KS‐PGs) from cerebrum (CB), cerebellum (CL) and brainstem (BS) of young sheep brain were isolated, purified and characterized. The amount of KS‐PGs in CL was significantly lower than that in CB and BS. KS‐PGs were characterized by increased extent of glycosylation and heterogeneity of KS chains in CL. Western blot analyses demonstrated the presence of the KS‐PGs phosphacan, SV2A and SV2B isoforms of synaptic vesicle proteoglycan in all three areas of the young sheep brain. Phosphacan predominated in BS and CB, showing significant molecular heterogeneity. SV2A and SV2B were found in two forms of high and low molecular sizes according to their extent of glycosylation in sheep brain. SV2A predominated in CL, where forms with very high molecular sizes were detected. Immunohistochemical examination revealed that SV2A was localized in the extracellular matrix of both gray and white matter. In contrast, phosphacan and SV2B were mainly localized in the white matter in all brain regions. The results of the present study demonstrated that KS‐PGs are present in the three areas of the sheep brain, showing significant variations in their content, structure and localization among the distinct areas. These differences may be important for the physiology of the brain. Copyright © 2008 John Wiley & Sons, Ltd. 相似文献
122.
Interfacial wave characteristics were studied experimentally in horizontal oil–water pipe flows during stratified flow and at the transition to dual continuous flow, where drops of one phase appear into the other (onset of entrainment). The experimental investigations were carried out in a stainless steel test section with 38 mm ID with water and oil (density 828 kg/m3and viscosity 5.5 mPas) as test fluids. Wave characteristics were obtained with a high speed video camera and a parallel wires conductivity probe that measured the instantaneous fluctuations of the interface. Experiments were conducted at 2 m and at 6 m from the inlet. Visual observations revealed that no drops are formed when interfacial waves are absent. It was also found that waves have to reach a certain amplitude before drops can detach from their crests. Wave amplitudes are increased as the superficial velocities of both phases increase. In the stratified region, the mean wave amplitude decreases by increasing the oil–water input ratio while mean wavelength increases as the slip velocity between the two-phase decreases. At the onset of entrainment, the mean amplitude and length are found to be a function of the relative velocity between the oil and water layers and of the turbulence in each layer. 相似文献
123.
A. Petrou M.C. Smith C.H. Perry J.M. Worlock J. Warnock R.L. Aggarwal 《Solid State Communications》1985,55(10):865-868
We have investigated the photoluminescence associated with residual manganese acceptors in n-type, modulation doped, GaAs/AlGaAs multiple quantum wells. In a magnetic field the luminescence breaks into discrete lines attributed to transitions between conduction band Landau levels and manganese acceptor states. The polarization of the luminescence was studied as function of magnetic field. A simple model based on the spin exchange interaction between the holes and the manganese ions successfully describes the polarization data. 相似文献
124.
Panagiota Daskalopoulos Manuel del Pino 《Transactions of the American Mathematical Society》2002,354(4):1583-1599
We study the solvability of the Cauchy problem for the nonlinear parabolic equation
when in , with a given nonnegative function. It is known from earlier works of the authors that the asymptotic radial growth , for the spherical averages of is critical for local solvability, in particular ensuring it if is radially symmetric. We show that if the initial data behaves in polar coordinates like , for large with nonnegative and -periodic, then the following holds: If vanishes on some interval of length 0$">, then there is no local solution of the initial value problem. On the other hand, if such an interval does not exist, then the initial value problem is locally solvable and the time of existence can be estimated explicitly.
when in , with a given nonnegative function. It is known from earlier works of the authors that the asymptotic radial growth , for the spherical averages of is critical for local solvability, in particular ensuring it if is radially symmetric. We show that if the initial data behaves in polar coordinates like , for large with nonnegative and -periodic, then the following holds: If vanishes on some interval of length 0$">, then there is no local solution of the initial value problem. On the other hand, if such an interval does not exist, then the initial value problem is locally solvable and the time of existence can be estimated explicitly.
125.
Panagiota Sofou Eugenia Panou-Pomonis Vassilios Tsikaris Maria Sakarellos-Daitsiotis Fernando Formaggio 《Tetrahedron》2004,60(12):2823-2828
A proline-rich [60]fullerene peptide was synthesized by use of (i) a 1,3-dipolar cycloaddition of an N-substituted glycine derivative to [60]fullerene, (ii) esterification of the isolated alcohol with the C-terminal amino acid of the desired peptide sequence, and finally (iii) coupling of the remaining hexapeptide to give the final product 8 as a TFA salt, with oxidized methionine. Product 8 was found to be biologically active against sera from MCTD and SLE patients (ELISA experiment). 相似文献
126.
Athinoula L. Petrou 《Transition Metal Chemistry》1993,18(5):462-466
Summary Binuclear complexes of dihydrocaffeic, caffeic and ferulic acids with vanadium were prepared and studied. The suggested square-pyramidal structures with catecholic-type coordination are supported by various spectroscopic, magnetic and thermogravimetric data. 相似文献
127.
Physicochemical study of novel organoclays as heavy metal ion adsorbents for environmental remediation 总被引:3,自引:0,他引:3
Stathi P Litina K Gournis D Giannopoulos TS Deligiannakis Y 《Journal of colloid and interface science》2007,316(2):298-309
Four organic-modified clays based on a SWy-2 montmorillonite were prepared by embedding ammonium organic derivatives with different chelating functionalities (NH(2), COOH, SH or CS(2)) in the interlayer space of montmorillonite. Organic molecules such as (a) hexamethylenediamine, (b) 2-(dimethylamino)ethenethiol, (c) 5-aminovaleric acid and (d) hexamethylenediamine-dithiocarbamate were used for the clay modification in order to study the effect of the chelating functionality on heavy metal ions binding from aqueous solutions. The organoclays were characterized by powder X-ray diffraction (XRD), infrared (FTIR) and NMR spectroscopies. The experimental data showed that the organic molecules are intercalated into the interlamelar space with the long dimension parallel to the clay sheets. Their sorbing properties were evaluated for the removal of heavy metals, Pb, Cd and Zn, from aqueous solutions as a function of the pH. When compared with the unmodified SWy-2 montmorillonite, the modified clays show significant improvement in terms of sorbing selectivity as well as of metal loading capacity. The fit to adsorption data by a Surface Complexation Model shows that the intercalated molecules act as specific binding sites in the clay. These contribute additional sorption capacity which is additive to the variable charge edge-sites of the clay in competition with the permanent charge sites. 相似文献
128.
Papadopoulou P Tzakou O Vagias C Kefalas P Roussis V 《Molecules (Basel, Switzerland)》2007,12(5):997-1005
Four new beta-orcinol metabolites, hypotrachynic acid (1), deoxystictic acid (2), cryptostictinolide (3) and 8'-methylconstictic acid (4) along with the metabolites 8'-methylstictic acid (5), 8'-methylmenegazziaic acid (6), stictic acid (7), 8'-ethylstictic acid (8) and atranorin (9), that have been previously described, were isolated for the first time from the tissue extracts of the lichen Hypotrachyna revoluta (Fl?rke) Hale. The structures of the new metabolites were elucidated on the basis of extensive spectroscopic analyses. Radical scavenging activity (RSA) of the metabolites isolated in adequate amounts, was evaluated using luminol chemiluminescence and comparison with Trolox. 相似文献
129.
In an effort to gain enhancement of selectivity in reversed-phase liquid chromatography, retention was tuned in this study by introducing short and medium straight-chained-length alkanol additives (methanol (MeOH), ethanol, 1-propanol, 1-butanol, 1-pentanol, 1-hexanol or 1-heptanol) at low concentrations in mobile phases containing MeOH as the main organic solvent. A six-parameter retention model considering simultaneously the contents of the main organic modifier and of the alcohol additive as well as of the number of alkyl chain of additive was developed by a direct combination of equations expressing separately a linear dependence of the retention upon each of these factors. The effectiveness of the above model was tested in the retention prediction of a mixture of six alkylbenzenes under isocratic conditions with mobile phases containing as an additive any member of the homologues series of alkanols (with 1-7 carbon atoms) at different low concentrations in a wide range of MeOH-water mixtures. The prediction was excellent in all cases even when the alkanol additives used in experiments for the fitting procedure are different than those used in chromatographic runs done for testing the prediction ability of the proposed model. 相似文献
130.
Kalogianni DP Boutsika LM Kouremenou PG Christopoulos TK Ioannou PC 《Analytical and bioanalytical chemistry》2011,400(4):1145-1152
Presently, there is a growing interest in the development of lateral flow devices for nucleic acid analysis that enable visual
detection of the target sequence (analyte) while eliminating several steps required for pipetting, incubation, and washing
out the excess of reactants. In this paper, we present, for the first time, lateral flow tests exploiting oligonucleotide-functionalized
and antibody-functionalized carbon nanoparticles (carbon nano-strings, CBNS) as reporters that enable confirmation of the
target DNA sequence by hybridization. The CBNS reporters were applied to (a) the detection of PCR products and (b) visual
genotyping of single nucleotide polymorphisms in human genomic DNA. Biotinylated PCR product was hybridized with a dA-tailed
probe. In one assay configuration, the hybrid is captured at the test zone of the strip by immobilized streptavidin and detected
by (dT)
30
-CBNS. In a second configuration, the hybrids are captured from immobilized (dA) strands and detected by antibiotin-CBNS. As low as 2.5 fmol of amplified DNA can be detected. For visual genotyping, allele-specific primers with a 5′ oligo(dA)
segment are extended by DNA polymerase with a concomitant incorporation of biotin moieties. Extension products are detected
either by (dT)
30
-CBNS or by antibiotin-CBNS. Only three cycles of extension reaction are sufficient for detection. No purification of the PCR products or the extension
product is required. 相似文献