Andrea Cagnini  Ilaria Palchetti  Marco Mascini  Anthony P. F. Turner 《Mikrochimica acta》1995,121(1-4):155-166

Plastic disposable choline biosensors based on ruthenized-carbon screen-printed electrodes were prepared and their use for monitoring organophosphorus pesticides and carbamates is described. The presence of 0.5% ruthenium on activated carbon mixed to form a simple graphite-based ink for the working electrode surface increased the sensitivity towards hydrogen peroxide. The choline biosensor is based on such an electrode coupled with choline oxidase immobilized by adsorption and was used to detect the inhibition effect of carbamates and organophosphorus pesticides on acetylcholinesterase. With the optimized procedure described (pH, buffer composition, incubation time, substrate concentration), concentrations of pesticides (Carbofuran) as low as 1 nM could be detected.  相似文献   

    

Daniele Di Corcia  Veronica Morra  Marco Pazzi  Marco Vincenti 《Biomedical chromatography : BMC》2010,24(4):358-366

A fast screening protocol was developed and validated for the simultaneous determination of 15 β₂‐agonists in human urine (bambuterol, cimbuterol, clenbuterol, fenoterol, formoterol, isoproterenol, mapenterol, metaproterenol, procaterol, ractopamine, ritodrine, salbutamol, salmeterol, terbutaline, tulobuterol). The overall sample processing includes deconjugation with enzyme hydrolysis, liquid–liquid extraction, followed by derivatization of the extract and detection of β₂‐agonists trimethylsilyl‐derivatives by fast‐gas chromatography/electron impact–mass spectrometry (fast‐GC/EI‐MS). Sample extraction and derivatization were optimized with the purpose of improving recoveries and reaction yields for a variety of analytes with different structures simultaneously, while keeping the procedure simple and reliable. Validation parameters were determined for each analyte under investigation, including selectivity, linearity, intra‐ and inter‐assay precision, extraction recoveries and signal to noise ratio (S/N) at the lowest calibration level. Fast‐GC/MS sequences, based on the use of short columns, high carrier‐gas velocity and fast temperature ramping, allow considerable reduction of the analysis time (7 min), while maintaining adequate chromatographic resolution. The overall GC cycle time was less than 9 min, allowing a processing rate of 6 samples/h. High MS‐sampling rate, using a benchtop quadrupole mass analyzer, resulted in accurate peak shape definition under both scan and selected ion monitoring modes, and high sensitivity in the latter mode. The method was successfully tested on real samples arising from clinical treatments. Copyright © 2009 John Wiley & Sons, Ltd.  相似文献   

    

Yasemin Yuksel Durmaz  Marco Sangermano  Yusuf Yagci 《Journal of polymer science. Part A, Polymer chemistry》2010,48(13):2862-2868

A novel method for surface modification of UV‐cured epoxy network was described. Photoinitiated cationic copolymerization of a bisepoxide, namely 3,4‐epoxy cyclohexylmethyl 3,4‐epoxycyclohexanecarboxylate (EEC) with epibromohydrine (EBH) by using a cationic photoinitiator, [4‐(2‐methylpropyl)phenyl]4‐methylphenyl‐iodonium hexafluorophosphate, in propylene carbonate solution was studied. The real‐time Fourier transform infrared spectroscopic, gel content determination and thermal characterization studies revealed that both EEC and EBH monomers take part in the polymerization and epoxy network possessing bromomethyl functional groups was obtained. The bromine functions of the cured product formed on the glass surface were converted to azide functionalities with sodium azide. Independently prepared alkyne functional poly(ethylene glycol) (PEG) was subsequently anchored to azide‐modified epoxy surface by a “click” reaction. Surface modification of the network through incorporation of hydrophilic PEG chain was evidenced by contact angle measurements. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 2862–2868, 2010  相似文献   

    

Antonio Tricoli  Marco Righettoni  Alexandra Teleki 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2010,122(42):7796-7825

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Mohammad&#x;S. Alam  Michael Stocker  Klaus Gieb  Paul Müller  Marco Haryono  Katja Student  Andreas Grohmann 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2010,122(6):1178-1182

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Jyoti Rajput Dr.  Dennis B. Rahbek Dr.  Lars H. Andersen Prof.  Amiram Hirshfeld Dr.  Mordechai Sheves Prof.  Piero Altoè Dr.  Giorgio Orlandi Prof.  Marco Garavelli Dr. 《Angewandte Chemie (International ed. in English)》2010,49(10):1790-1793

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Stephan H. von Reuß  Marco Kai  Birgit Piechulla Prof. Dr.  Wittko Francke Prof. Dr. 《Angewandte Chemie (International ed. in English)》2010,49(11):2009-2010

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Marco Affronte  Ian Casson  Marco Evangelisti  Andrea Candini  Stefano Carretta  Christopher A. Muryn  Simon J. Teat  Grigore A. Timco  Wolfgang Wernsdorfer  Richard E. P. Winpenny 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2005,117(40):6585-6585

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Olimpia Mamula  Marco Lama  Shane G. Telfer  Asao Nakamura  Reiko Kuroda  Helen Stoeckli‐Evans  Rosario Scopelitti 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2005,117(17):2583-2587

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Affronte M  Casson I  Evangelisti M  Candini A  Carretta S  Muryn CA  Teat SJ  Timco GA  Wernsdorfer W  Winpenny RE 《Angewandte Chemie (International ed. in English)》2005,44(40):6496-6500

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