The aim of this study was to measure the shear modulus of the vocal fold in a human hemilarynx, such that the data can be related to direction of applied stress and anatomical context. Dynamic spring rate data were collected using a modified linear skin rheometer using human hemilarynges, and converted to estimated shear modulus via application of a simple shear model. The measurement probe was attached to the epithelial layer of the vocal fold cover using suction. A sinusoidal force of 3g was applied to the epithelium, and the resultant displacement logged at a rate of 1kHz. Force measurement accuracy was 20microg and position measurement accuracy was 4microm. The force was applied in a transverse direction at the midmembranous point between the vocal process and the anterior commissure. The shear modulus of the three female vocal folds ranged from 814 to 1232Pa. The shear modulus of the three male vocal folds ranged from 1021 to 1796Pa. These data demonstrate that it is possible to obtain estimates for the shear modulus of the vocal fold while preserving anatomical context. The modulus values reported here are higher than those reported using parallel plate rheometry. This is to be expected as the tissue is attached to surrounding structures, and is under natural tension. 相似文献
Microalgal lipids were separated into two fractions, triacylglycerols (TAGs) and free fatty acids (FFAs), by solid-phase extraction employing sodium carbonate as the sorbent and dichloromethane (20% by volume) in n-hexane as the extracting solvent. The TAG fraction was then saponified, followed by acidification, extraction and tert-butyldimethylsilyl esterification. The FFA fraction was directly acidified, extracted and derivatized. From the lipid extracts of eight microalgal species examined, a total of 13 fatty acids were detected in the TAG fractions and nine were found in the FFA fractions, with at much higher total TAG content in all microalgae. Oleic acid was the most prominent fatty acid in three species, α-linolenic acid was more abundant in two others, and palmitic acid was present in highest concentration in the remaining three species. 相似文献
Quick on the uptake : Palladium nanoparticles were fabricated simply by immersing {[Zn3(ntb)2(EtOH)2]?4 EtOH}n ( 1 ) in an MeCN solution of Pd(NO3)2 at room temperature, without any extra reducing agent. 3 wt % PdNPs@[ 1 ]0.54+(NO3?)0.54 significantly increase H2 uptake capacities, both at 77 K and 1 bar and at 298 K and high pressures (see picture, red curve) compared to [Zn3(ntb)2]n (black). ntb=4,4′,4′′‐nitrilotrisbenzoate.
A model black hole, holding a ‘quantum core’ characterized by the Planck order matter density, is revisited here. Based on the quantum improved Newton’s potential drawn out of the loop quantum cosmology we propose a Schwarzschild class, quantum improved black hole line-element that upholds the existence of Planck-dense quantum matter core. Causality is kept preserved in this proposal. Quite in a natural way the quantum core emerges closely homogeneous in its interior matter distribution. The radius of the quantum core turns out to be necessarily proportional to one-third power of the black hole mass. Hawking process of black hole evaporation leads to a shrinking quantum core, and as the mass of black hole approaches near about the Planck mass, the rate of evaporation diminishes rapidly and eventually leaves a cold remnant having a Planck order mass. Proposed model supports the standard quantum geometrical logarithmic correction to black hole entropy-area law. 相似文献
We investigate the performance of unsteady Reynolds-averaged Navier–Stokes (URANS) computation and various versions of detached eddy simulation (DES) in resolving coherent structures in turbulent flow around two cubes mounted in tandem on a flat plate at Reynolds number (Re) of 22,000 and for a thin incoming boundary layer. Calculations are carried out using four different coherent structure resolving turbulence models: (1) URANS with the Spalart–Allmaras model; (2) the standard DES [Spalart, P.R., Jou, W.H., Strelets, M., Allmaras, S.R., 1997. Comments on the feasibility of LES for wings, and on a hybrid RANS/LES approach. In: Liu, C., Liu, Z., (Eds.), Advances in DNS/LES. Greyden Press, Columbus, OH]; (3) the Delayed DES (DDES); and (4) the DES with a low-Re modification (DES-LR) [Spalart, P., Deck, S., Shur, M., Squires, K., Strelets, M., Travin, A., 2006. A new version of detached eddy simulation, resistant to ambiguous grid densities. Theor. Comput. Fluid Dyn. 20 (3), 181–195]. The grid sensitivity of the computed solutions is examined by carrying out simulations on two successively refined grids. The computed results for all cases are compared with the experimental measurements of Martinuzzi and Havel [Martinuzzi, R., Havel, B., 2000. Turbulent flow around two interfering surface-mounted cubic obstacles in tandem arrangement. ASME J. Fluids Eng. 122, 24–31] for two different cube spacings. All turbulence models reproduce essentially identical separation of the approach thin boundary layer and yield an unsteady horseshoe vortex system consisting of multiple vortices in the leading edge region of the upstream cube. Significant discrepancies between the URANS and all DES solutions are observed, however, in other regions of interest such as the shear layers emanating from the cubes, the inter-cube gap and the downstream wake. Regardless of the grid refinement, URANS fails to capture key features of the mean flow, including the second horseshoe vortex in the upstream junction and recirculating flow on the top surface of the downstream cube for the large cube spacing, and underestimates significantly turbulence statistics in most regions of the flow for both cases. On the coarse mesh, all three DES approaches appear to yield very similar results and fail to reproduce the second horseshoe vortex. The standard DES and DDES solutions obtained on the fine meshes are essentially identical and both suffer from premature switching to unresolved DNS, due to the mis-interpretation of grid refinement as wall proximity, which leads to spurious vortices in the inter-cube region. Numerical solutions show that the low-Re modification (DES-LR) is critical prerequisite in DES on the ambiguously fine – not fine enough for full LES – mesh to prevent excessive nonlinear drop of the subgrid eddy viscosity in low cell-Re regions like in the inter-obstacle gap. Mean flow quantities and turbulence statistics obtained with DES-LR on the fine mesh are in good overall agreement with the measurements in most regions of interest for both cases. 相似文献
We consider a collapsing spherically symmetric inhomogeneous dust cloud in higher dimensional space-time. We show that the
central singularity of collapse can be a strong curvature or a weak curvature naked singularity depending on the initial density
distribution. 相似文献
Inclusion studies for metal-organic open-frameworks, [Ni(C10}H24N4)(H2O)2]3[BTC]2·24H2O (1) and [Ni(C10H26N6)]3 [BTC]2·18H2O(2) (BTC3- = 1,3,5-benzenetricarboxylate) with various organic andinorganic guest molecules have been carried out. 1 is the previously reportedmolecular floral lace with 1-D channels, where positively charged macrocyclic layersand negatively charged BTC3- layers are alternately packed by hydrogen bondinginteractions. 2 is assembled in this study from nickel(II) hexaazamacrocyclic complexcontaining methyl pendant arms and BTC3-. The X-ray structure of 2 shows thatthe nickel(II) complex and BTC3- form a 2-D coordination polymer. The XRPD patternsof 2 indicate that framework of 2 is slightly deformed upon removal of waterguest molecules but restored upon rebinding of water. The host solid 1 binds MeOHin toluene, and 1,3,5-trihydroxybenzene (THB) and 4-hydroxyacetophenone (HAP) in EtOH/toluene (v/v = 1/4) solutions. The binding constants (Kf) of 1 forMeOH, THB, and HAP are 66.4 M-1, 259 M-1, and 13.9 M-1,respectively. In the range of high concentration of the guest, however, the host showsvarious binding curves depending upon the types of guest. It binds PhOH in toluene,showing a sigmoid curve. It also binds transition metal complexes such as[Cu(NH3)4](ClO4)2, [Cu(ethylenediamine)2](ClO4)2,[Cu(histamine)2](ClO4)2, and[Cu(N,N'-bis(3-aminopropyl)ethylenediamine)](ClO4)2 in MeCN, with Kf values of 645 M-1, 9.52 M-1, 37.2 M-1, and 6.00 M-1,respectively. The host solid 2 binds selectively PhOH over PhCl and PhBr, showing that hydrogen bonding interaction between the host and guest plays an important role in the selectivity. 相似文献
Summary Early diagnosis of fatty acid oxidation (FAO) disorders is important to reduce severe morbidity and mortality. Although analysis
of plasma free fatty acids (FFAs) is frequently performed using stable isotope-dilution gas chromatography-mass-spectrometry
(GC-MS), there are institutions where the required instrumentation is not available to support a rapid work-up of acutely
ill patients. For this reason, we have developed a novel cyanomethyl derivatization method for FFAs which is followed by GC
analysis of the resulting esters using nitrogen-phosphorus detector (NPD) for the rapid diagnosis of mitochondrial fatty acid
oxidation disorders.
FFAs were extracted from plasma and derivatized to the cyanomethyl ester by heating with bromoacetonitrile at 60°C for 30
min GC-NPD analysis was then performed.
The mean recoveries of C6:0-C18:0FFAs were between 87% abd 96%. The method detection limits (S/N=3) were 0.1–0.5 ng for C6:0-C14:0
FFAs, and 0.001–0.01 ng for C16:0-C18:0 FFAs.
We succesfully performed differential diagnosis of representative FAO disorders from the confimed patient's plasmas. This
simple method offers cost-effective and time-saving alternative to GC-MS for the biochemical diagnosis of selected FAO disorders. 相似文献