The Cardbox/Cardview Database for residues in live animals and their products

The growing interest of the public opinion in environmental problems has resulted in the establishment of national and international norms and regulations. Among others, the protection of human health requires the constant monitoring of several groups of potentially toxic substances as well as of their residues in live animals and their products. For this reason in recent years the European Commission decided to establish four Community Reference Laboratories (CRLs) for the analysis of residues in products of animal origin. The current mandate and operative conditions of the CRLs are described in the Council Directive 96/23/EC of 29 April 1996, which has the goal of promoting and supporting the harmonization of approaches among National Reference Laboratories (NRLs). In this context the CRL at the Istituto Superiore di Sanità of Rome is responsible for chemical elements, organochlorine compounds (including PCBs), carbamates, pyrethroids and organophosphorus compounds. The supply of updated information on the literature of relevance to the tasks of the NRLs is one of the duties of the Rome CRL. To this end, the so-called Cardbox Database has been set up, which for the time being is fully operative for chemical elements and organochlorine compounds.     

Motorcycle brake squeal: experimental and numerical investigation on a case study

Since, nowadays, the NVH performance of a vehicle is one of the most important priorities for the market, the noise radiating from disc brakes is considered a source of considerable discomfort and customer dissatisfaction. Squeal is an example of noise, caused by vibrations induced by friction forces, in which the vibration modes of the brake disc are coupled to those of the friction pads or of the caliper. In this work a case study, in which the squeal phenomenon was detected after changing the supplier of the disc pads, is presented. A test bench was purposely developed to investigate the squeal phenomenon; tests at different rotating speeds and pressures in the brake circuit were carried out employing different friction pads. The experimental apparatus appeared capable of reproducing the phenomenon observed in real practice and to investigate the effect of operating parameters and different components on the onset of instability. Friction tests and geometric analysis of the friction pads were also carried out to complete the investigation. At the same time, a finite element (FE) complex eigenvalue parametric analysis was performed on the brake assembly. The different propensity of the pads to squeal was attributed to differences in their geometry and in their friction coefficients. The FE analysis, confirmed the experimental observations and indicated possible design improvements to increase the stability of the system.     

Trace elements in bovine muscle: an ongoing project for a new certified reference material

In the framework of the activities of the Community Reference Laboratory (CRL) for residues at the Istituto Superiore di Sanità (ISS) of Rome, a number of proficiency tests were performed in order to assess and improve, wherever necessary, the quality of regulatory residue analysis within the EU. In this context, a pilot study was undertaken with the purpose of ascertaining the feasibility of a project for the certification of a new reference material for trace elements in bovine muscle. On behalf of the ISS, approximately 70 kg of bovine muscle, collected at a slaughterhouse in Geel (Belgium), were freeze-dried, homogenized, stabilized and bottled under argon atmosphere by the Institute for Reference Materials and Measurements, Joint Research Centre, European Commission (IRMM-JRC-EC). Preliminary analyses were carried out with Quadrupole (Q) and High Resolution (HR) Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to evaluate the content of As, Cd, Cu and Pb, whereas the Flow Injection Mercury System (FIMS) was used to quantify Hg. Digestion of the matrix was achieved by acid-assisted microwave irradiation. The mean values of experimentally obtained data were the following (in nanograms per gram): As, 24.2±1.2; Cd, 5.48±0.29; Cu, 4765±95; Hg, 3.37±0.73; and Pb, 184±7. Subsequently, 16 National Reference Laboratories (NRLs) for residues in the Member States plus the Norwegian one and eight Italian public laboratories were accepted to participate in the project and received one bottle of the freeze-dried bovine muscle. The laboratories were requested to perform three different analytical runs (in three different days), each consisting of three individual measurements. Preliminary results showed good agreement and fair evidence for homogeneity of the entire mass, thus setting the stage for the future certification of this candidate CRM.     

Antimicrobial Effect and Cytotoxic Evaluation of Mg-Doped Hydroxyapatite Functionalized with Au-Nano Rods

Hydroxyapatite (HA) is the main inorganic mineral that constitutes bone matrix and represents the most used biomaterial for bone regeneration. Over the years, it has been demonstrated that HA exhibits good biocompatibility, osteoconductivity, and osteoinductivity both in vitro and in vivo, and can be prepared by synthetic and natural sources via easy fabrication strategies. However, its low antibacterial property and its fragile nature restricts its usage for bone graft applications. In this study we functionalized a MgHA scaffold with gold nanorods (AuNRs) and evaluated its antibacterial effect against S. aureus and E. coli in both suspension and adhesion and its cytotoxicity over time (1 to 24 days). Results show that the AuNRs nano-functionalization improves the antibacterial activity with 100% bacterial reduction after 24 h. The toxicity study, however, indicates a 4.38-fold cell number decrease at 24 days. Although further optimization on nano-functionalization process are needed for cytotoxicity, these data indicated that Au-NRs nano-functionalization is a very promising method for improving the antibacterial properties of HA.     

Sopra un sistema di equazioni funzionali nella teoria dell'informazione

Riassunto Si stabiliscono alcune proprietà riguardanti i valori al contorno delle soluzioni di un sistema di equazioni funzionali connesso con la teoria dell'informazione senza probabilità. Si ottiene poi in particolare una caratterizzazione per talune misure di informazione compositive a traccia shannoniana.

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Résumé On établit certaines propriétés de trace pour les solutions continues d'un système d'équations fonctionelles qu'on rencontre dans la théorie de l'information sans probabilité. D'après ?a on obtient en particulier une caractérisation de certaines mesures compositives à trace shannonienne.

     

Processi ergodici non stazionari e loro proprietà

Sunto Si stabilisce la condizione necessaria e sufficiente per l’equivalenza ad una costante della media empirica di una qualunque funzione misurabile, in un processo discreto Lavoro eseguito per la realizzazione del programma del gruppo n^o 9 del C. N. R. (1961–62).     

1,3-Dipolar cycloadditions of azomethine ylides with dipolarophiles. II. Synthesis of pyrrolizidines

Substituted pyrrolizidines have been synthesized by 1,3-dipolar cycloaddition of various dipolarophiles with the azomethine ylide generated by treatment of proline with benzaldehyde in dimethylsulfoxide. The regio- and stereochemical outcome of the reaction as well as the stability of the isomers obtained are discussed.