Partial purification and characterization of protease enzyme from Bacillus subtilis and Bacillus cereus

The aim of this experimental study was to isolate and partially purify protease enzyme from Bacillus cereus and Bacillus subtilis. Protease enzyme is obtained by inducing spore genesis of bacteria from Bacillus species in suitable nutrient plates. The partial purification was realized by applying, respectively, ammonium sulfate precipitation, dialysis, and DEAE-cellulose ion-exchange chromatography to the supernatant that was produced later. Optimum pH, optimum temperature, pH stability, and temperature stability were determined, as well as the effects of pH, temperature, substrate concentration, reaction time, and inhibitors and activators on enzyme activity. In addition, the molecular mass of the obtained enzyme was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The specific activity of partially purified enzyme from B. subtilis was determined to be 84 U/mg. The final enzyme preparation was eight-fold more pure than the crude homogenate. The molecular mass of the partially purified enzyme was found to be 45 kDa by using SDS-PAGE. The protease enzyme that was partially purified from B. cereus was purified 1.2-fold after ammonium sulfate precipitation. The molecular mass of the partially purified enzyme was determined to be 37 kDa by using SDS-PAGE.     

(2‐Hydroxy­ethyl)­hydrazinium(2+) dichloride

The crystal structure of the title compound, C₂H₁₀N₂O²⁺·2Cl⁻, is built up from one 2‐hydroxy­ethyl­hydrazinium(2+) cation and two Cl⁻ anions. The mol­ecular structure is stabilized by O—H⋯Cl and N—H⋯Cl hydrogen bonds. The crystal structure is stabilized by one N—H⋯O and three N—H⋯Cl inter­actions, and the three‐dimensional network of hydrogen bonds stabilizes the crystal packing. All five hydrazinium H atoms are involved in hydrogen bonds to Cl⁻ anions. The Cl⋯H contact distances range from 2.122 (15) to 2.809 (14) Å.     

Synthesis,crystal structure and catalytic activity for H2O2 disproportionation of the manganese(II) coordination polymer {[Mn(O2C(CF2)8CO2)(phen)2]H2O}n (phen = 1,10-phenanthroline)

The title polymeric complex {[Mn(O₂C(CF₂)₈CO₂)(phen)₂]H₂O}_n was synthesized through the reaction of 1,10-phenanthroline, perfluorosebacic acid and MnCO₃ · H₂O. The molecular structure was characterized by X-ray diffraction, elemental analysis, thermal gravimetry, IR and UV–Vis spectroscopy and its catalytic activity has been studied. X-ray structure analysis shows that each Mn(II) ion is octahedrally coordinated by two bidentate phenanthroline ligands and the carboxylate oxygen atoms from two symmetry related perfluorosebacate ligands, which are coordinated in cisoid positions. The structure consists of polymeric chains, with the perfluorosebacato ligand bridging the Mn(II) ions in a monodentate fashion. Crystallographic characterization shows a supramolecular structure involving hydrogen bonds, π–π and π-ring interactions. The catalytic results indicated that the complex has reasonably good activity towards the disproportionation of hydrogen peroxide into water and dioxygen in methanol and it does not exhibit saturation kinetics with the substrate. The initial reaction rates and their temperature and base dependencies were investigated by monitoring the dioxygen evolution. Kinetic studies revealed a first-order dependence on the catalyst concentration. Activation parameters have been calculated at 301 K.     

Efficient synthesis of some oxalacetic acid and pyruvic acid derivatives from the reactions of 2,3-furandiones with 2-phenylindole

Oxalacetic acid and pyruvic acid derivatives have been synthesized efficiently in high yields by the treatment of 4-ethoxycarbonyl-5-phenyl-2,3-furandione and 4-benzoyl-5-phenyl-2,3-furandione with 2-phenylindole at room temperature and converted to simple derivatives such as an ester or a hydrazone.     

Studies on plasma-initiated polymerization of acrylamide in solid state and in solution

Plasma-initiated polymerization is applied to acrylamide both in solid state and in solution. The effect of the amount of water, in addition to certain operational parameters such as applied vacuum, post polymerization period, and temperature, are well verified. Characteristics of polymers are determined by IR spectroscopy and X-ray diffraction studies as well as by intrinsic viscosity determinations.     

Local molecular orbitals and hyper-susceptibility of TeO2 glass

The dielectric properties of the glassy telluria have been modeled and studied via the ab initio calculations of the linear- and hyper-polarizabilities of chain-like (TeO₂)_n clusters. By using the localized molecular orbitals approximation (GAMESS program), it is shown that their linear polarizability is mainly associated with the tellurium atom lone pairs and with the Te–O–Te bridges, whose contributions are comparable. On contrary, the bridge contributions unequivocally dominate the hyperpolarizability value (providing 75% of this) whereas the role of the lone pair on tellurium atoms is minimal (5%). The same estimations can be obtained for the relevant characteristics of TeO₂ glass.     

MHD mixed convective heat transfer flow about an inclined plate

Mixed convection heat transfer about a semi-infinite inclined plate in the presence of magneto and thermal radiation effects is studied. The fluid is assumed to be incompressible and dense. The nonlinear coupled parabolic partial differential equations governing the flow are transformed into the non-similar boundary layer equations, which are then solved numerically using the Keller box method. The effects of the mixed convection parameter R _i, the angle of inclination α, the magnetic parameter M and the radiation–conduction parameter R _d on the velocity and temperature profiles as well as on the local skin friction and local heat transfer parameters. For some specific values of the governing parameters, the results are compared with those available in the literature and a fairly good agreement is obtained.     

Conjugate heat transfer in thermally developing laminar flow with viscous dissipation effects

In this study, thermally developing laminar forced convection in a pipe including viscous dissipation and wall conductance is investigated numerically. The constant heat flux is assumed to be imposed at the outer surface of the pipe wall. The finite volume method is used. The distributions for the developing temperature and local Nusselt number in the entrance region are obtained. The dependence of the results on the Brinkman number and the dimensionless thermal conductivity are shown. The viscous heating effect on the wall is shown. Significant viscous dissipation effects have been observed for large Br.