The Hamiltonian of the guiding centre in an adiabatically variable electromagnetic field (for the relative variations of the field 1/H. dH/dtc, 1/E. dE/dtc) is derived in the present paper.The solution of the corresponding canonical equations is given for an axially symmetric stationary electromagnetic field. 相似文献
Potassium-silicate glass, 0.05K2O·0.95SiO2, was prepared by molecular dynamics under three different regimes (cooling rates, simulating box sizes) to study their influence to temperature regimes of volume fluctuations. Partial volume fluctuations (VF) were introduced with the help of Voronoi polyhedra tessellation. Dynamic and static fluctuations were suggested as a measure for time- and space-related fluctuations. Separation of the dynamic and static VFs anticipates glass transition. The glass transition was related with the change of the temperature course of the static VFs. The dynamic VFs changed their temperature regimes well below the glass transition and the transition temperature was related with the change of the transport regime. Glass transition cages the atoms but Voronoi polyhedra are definitely shaped well below the glass transition temperature. 相似文献
In this paper, we describe a novel technique for depositing metal nanoparticles (NPs) on a planar substrate whereby the NPs
are micro-patterned on the surface by a simple stamp-printing procedure. The method exploits the attractive force between
negatively charged colloidal metal NPs and positively-charged polyelectrolyte layers which have been selectively deposited
on the surface. Using this technique, large uniform areas of patterned metal NPs, with different plasmonic properties, were
achieved by optimisation of the stamping process. We report the observation of unusual fluorescence emission from these structures.
The emission was measured using epifluorescence microscopy. Fluorescence lifetime behaviour was also measured. Furthermore,
the μ-patterned NPs exhibited blinking behaviour under 469 nm excitation and the fluorescence spectrum was multi-peaked. It
has been established that the fluorescence is independent of the plasmon resonance properties of the NPs. As well as optimising
the novel NP μ-patterning technique, this work discusses the origin and characteristics of the anomalous fluorescence behaviour
in order to characterise and minimise this unwanted background contribution in the use of metal NPs for plasmonic enhancement
of fluorescence for optical biochip applications. 相似文献
Analytical and Bioanalytical Chemistry - In this work we describe a very fast and flexible method for fabrication of plasmon-supporting substrates with micro-patterning capability, which is... 相似文献
Respiratory infections are a real threat for humans, and therefore the pig model is of interest for studies. As one of a case for studies, Actinobacillus pleuropneumoniae (APP) caused infections and still worries many pig breeders around the world. To better understand the influence of pathogenic effect of APP on a respiratory system—lungs and tracheobronchial lymph nodes (TBLN), we aimed to employ matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI). In this study, six pigs were intranasally infected by APP and two were used as non-infected control, and 48 cryosections have been obtained. MALDI-TOF MSI and immunohistochemistry (IHC) were used to study spatial distribution of infectious markers, especially interleukins, in cryosections of porcine tissues of lungs (necrotic area, marginal zone) and tracheobronchial lymph nodes (TBLN) from pigs infected by APP. CD163, interleukin 1β (IL-1β) and a protegrin-4 precursor were successfully detected based on their tryptic fragments. CD163 and IL-1β were confirmed also by IHC. The protegrin-4 precursor was identified by MALDI-TOF/TOF directly on the tissue cryosections. CD163, IL-1β and protegrin-4 precursor were all significantly (p < 0.001) more expressed in necrotic areas of lungs infected by APP than in marginal zone, TBLN and in control lungs. 相似文献
Herein we evaluate the influence of an electric field on the coupling of two delocalized electrons in the mixed‐valence polyoxometalate (POM) [GeV14O40]8? (in short V14) by using both a t‐J model Hamiltonian and DFT calculations. In absence of an electric field the compound is paramagnetic, because the two electrons are localized on different parts of the POM. When an electric field is applied, an abrupt change of the magnetic coupling between the two delocalized electrons can be induced. Indeed, the field forces the two electrons to localize on nearest‐neighbors metal centers, leading to a very strong antiferromagnetic coupling. Both theoretical approaches have led to similar results, emphasizing that the sharp spin transition induced by the electric field in the V14 system is a robust phenomenon, intramolecular in nature, and barely influenced by small changes on the external structure. 相似文献
X-ray induced photoelectron spectroscopy has been used to study the influence of low-energy electron beam on the pristine potassium–lime–silicate glass surface prepared by fracturing in situ under ultrahigh vacuum. Relatively low-energy electron beam of 1600 eV with low-electron beam current density of 0.02–0.22 A/m2 and low-electron dose of 29–5200 C/m2 was used. The expected modified near-surface region thickness is in this case comparable with the mean sampling depth of the analytical tool. Therefore, possible changes and modifications due to electron irradiation could be recorded with high sensitivity. The freshly fractured glass surface was found to be significantly enriched with potassium, and slightly with calcium. As a consequence of the lowest electron dose irradiation used, the potassium signal substantially increased by a factor 1.24 relative to the value found for the fresh surface. For higher doses used, the potassium signal continuously increased with the dose to a maximum and decreased thereafter. This variation was accompanied with the qualitative opposite behaviour of calcium signal. The concentrations of the other elements present in the glass, oxygen and silicon, varied only slightly with the electron dose. They can be considered to be constant within experimental uncertainty. In agreement with experimental results, a model assuming mobility of only two most mobile cations, potassium and calcium, was suggested. The models assuming one layer and two layers on the bulk were developed. Their results reproduce well experimental findings: (i) the formation of a potassium-rich surface layer, and (ii) the opposite-like signal variation of calcium in comparison with potassium. 相似文献
For the design of a biohybrid structure as a ligand‐tailored drug delivery system (DDS), it is highly sophisticated to fabricate a DDS based on smoothly controllable conjugation steps. This article reports on the synthesis and the characterization of biohybrid conjugates based on noncovalent conjugation between a multivalent biotinylated and PEGylated poly(amido amine) (PAMAM) dendrimer and a tetrameric streptavidin‐small protein binding scaffold. This protein binding scaffold (SA‐ABDwt) possesses nM affinity toward human serum albumin (HSA). Thus, well‐defined biohybrid structures, finalized by binding of one or two HSA molecules, are available at each conjugation step in a controlled molar ratio. Overall, these biohybrid assemblies can be used for (i) a controlled modification of dendrimers with the HSA molecules to increase their blood‐circulation half‐life and passive accumulation in tumor; (ii) rendering dendrimers a specific affinity to various ligands based on mutated ABD domain, thus replacing tedious dendrimer–antibody covalent coupling and purification procedures.
We developed a new method for real‐time, three‐dimensional tracking of fluorescent particles. The instrument is based on a laser‐scanning confocal microscope where the focus of the laser beam is scanned or orbited around the particle. Two confocal pinholes are used to simultaneously monitor regions immediately above and below the particle and a feedback loop is used to keep the orbit centered on the particle. For moderate count rates, this system can track particles with 15 nm spatial resolution in the lateral dimensions and 50 nm in the axial dimension at a temporal resolution of 32 ms. To investigate the interaction of the tracked particles with cellular components, we have combined our orbital tracking microscope with a dual‐color, wide‐field setup. Dual‐color fluorescence wide‐field images are recorded simultaneously in the same image plane as the particle being tracked. The functionality of the system was demonstrated by tracking fluorescent‐labeled artificial viruses in tubulin‐eGFP expressing HUH7 cells. The resulting trajectories can be used to investigate the microtubule network with super resolution.相似文献
The antibandwidth problem consists of placing the vertices of a graph on a line in consecutive integer points in such a way that the minimum difference of adjacent vertices is maximised. The problem was originally introduced in [J.Y.-T. Leung, O. Vornberger, J.D. Witthoff, On some variants of the bandwidth minimisation problem, SIAM Journal of Computing 13 (1984) 650-667] in connection with the multiprocessor scheduling problems and can also be understood as a dual problem to the well-known bandwidth problem, as a special radiocolouring problem or as a variant of obnoxious facility location problems. The antibandwidth problem is NP-hard, there are a few classes of graphs with polynomial time complexities. Exact results for nontrivial graphs are very rare. Miller and Pritikin [Z. Miller, D. Pritikin, On the separation number of a graph, Networks 19 (1989) 651-666] showed tight bounds for the two-dimensional meshes and hypercubes. We solve the antibandwidth problem precisely for two-dimensional meshes, tori and estimate the antibandwidth value for hypercubes up to the third-order term. The cyclic antibandwidth problem is to embed an n-vertex graph into the cycle Cn, such that the minimum distance (measured in the cycle) of adjacent vertices is maximised. This is a natural extension of the antibandwidth problem or a dual problem to the cyclic bandwidth problem. We start investigating this invariant for typical graphs and prove basic facts and exact results for the same product graphs as for the antibandwidth. 相似文献