Electrochemical Monitoring of Methotrexate Anticancer Drug in Human Blood Serum by Using in situ Solvothermal Synthesized Fe3O4/ITO Electrode

Here, we reported on a one‐step fabrication of magnetite Fe₃O₄ nanoparticles/indium tin oxide (ITO) electrode based on the direct growing of Fe₃O₄ nanoparticles on the ITO surface by using a solvothermal process. The modified electrode was used as electrochemical methotrexate (MTX) biosensor with high sensitivity based on cyclic voltammetry and square wave voltammetry techniques. The results demonstrated a linear relationship between the MTX concentration and its oxidation current peak over a wide range from 10^?5 to 10^?14 mole/L with a limit of detection of 0.4×10^?15 M based on the square wave voltammetry (SWV) technique. In addition, Fe₃O₄/ITO electrode showed a good capability for measuring very low concentrations of MTX drug dissolved in human serum solution. Also, Fe₃O₄/ITO electrode was used for detecting MTX in blood serum samples collected from patients after their treatment with MTX. The prepared electrode showed the higher sensitivity that higher than the Viva‐E instrument, which opens the door for developing a cheap, simple and higher sensitive MTX sensor.     

Densitometric Kinetic Stability Studies of Some Plant Extracts Using Different Regression Methods

JPC – Journal of Planar Chromatography – Modern TLC -     

Biometric identification using local iterated function

Biometric identification protocol has been received an increasing interest recently. It is a process that determines person identity by making use of their biometric features. A new biometric identification method is presented in this paper based on partial self-similarity that used to identify features within fingerprint images. This approach is already used in Fractal Image Compression (FIC) due to their ability to represent the images by a limited number of affine transformations, and its variation of scale, translation or rotation. These features give the recognition process high impact and good performance. To process data in a fingerprint image, it first converted into digital format using Optical Fingerprint Reader (OFR). The verification process is done by comparing these data with the server data. The system analysis shows that the proposed method is efficient in terms of memory and time complexity.     

Supramolecular chemosensor for selective detection of iron in aqueous medium

We have successfully developed a ‘turn-on’ colorimetric chemosensor for Fe³⁺ based on 1,10-phenanthroline. An amide derivative of 1,10-phenanthroline 4 was developed for the selective recognition of Fe³⁺ over Co²⁺, Cr³⁺, Cu²⁺, Mn²⁺, Ni²⁺, Ag⁺ and Zn²⁺ and could measure Fe³⁺ concentration in the range of 15–210 μM by UV–vis spectroscopy. Moreover, the addition of Fe³⁺ to a colourless solution of 4 turned its colour to light pink, indicating that 4 is capable of detecting Fe³⁺ by the naked eye. Compound 4 exhibits a major absorption band centred at 268 which shifted to 278 nm after addition of Fe³⁺, and a shoulder band around 514 nm was also observed. The complexation of Fe³⁺ with 4 was analysed at a different pH and favourable binding was observed at pH 6.2.     

Synthesis,characterisation and supramolecular interaction of Rh-biphenylic-imidazole-phenanthroline with antibiotics

A novel rhodium biphenylic imidazole phenanthroline metal-organic complex (BIP-MC) has been synthesised and characterised as a stable supramolecule. The structure of compound was established on the basis ESI, ¹H NMR and UV–vis spectroscopic data. The selectivity of BIP-MC as a new fluorescent chemosensor for various antibiotics has been explored. The supramolecular interaction of amoxicillin with BIP-MC enhanced the fluorescence activity of BIP-MC. A linear response of the sensor was observed in the measuring ranges of excitation 240–298 nm and emission 290–360 nm with detection limits of up to 10 μg/ml at an optimum pH 8.0. Based on the observations made here, a new quantitative method for the determination of this drug in synthetic samples without the use of separation of matrix is developed. It is also inferred that the possible fluorescence enhancement is due to the formation of exciplex between the BIP-MC and amoxicillin. These finding will thus help in pharmacokinetics studies of drugs. The sensor was used for the direct assay of amoxicillin antibiotic in commercial pharmaceutical preparations.     

Building block synthesis of predominantly (E) symmetrical and unsymmetrical 1,2-difluorostilbenes from 1,2-dibromo-1,2-difluoroethene

An efficient synthesis of predominantly (E) symmetrical or unsymmetrical 1,2-difluorostilbenes based on the Suzuki–Miyaura palladium-catalyzed cross-coupling reaction of arylboronic acids with predominantly (E)-1,2-dibromo-1,2-difluoroethene in the presence of Cs₂CO₃ in toluene is described. The reaction preserved the stereochemistry of the building block and performed in good yield independently of the electron-withdrawing or electron-donating character of the substituents.     

Survey of Phenolic Acids,Flavonoids and In Vitro Antioxidant Potency Between Fig Peels and Pulps: Chemical and Chemometric Approach

In the present study, chromatic coordinates, phenolic acids, flavonoids and antioxidant capacity assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate (ABTS) and lipid peroxidation inhibition capacity (LPIC) essays and their relative IC50 were investigated in 25 fig cultivars growing in Morocco. The aims of this study were to determine (i) the variation in these compounds among light and dark-colored cultivars, (ii) their partitioning between fruit peel and pulp and (iii) to display network connections among these variables. Twelve phenolic compounds (PCs) were isolated in peel extract versus eight in pulp samples. Anthocyanins, mainly cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the predominant compounds in peels, where the mean concentrations were 75.90 ± 18.76 and 77.97 ± 18.95 µg/g dw, respectively. On the other hand, (−)-epicatechin and cyanidin-3-O-rutinoside were the major compounds in the pulp extracts, where the mean values were 5.23 ± 4.03 and 9.01 ± 5.67 µg/g dw, respectively. A two-dimensional hierarchically clustered heatmap was applied to the dataset to explore correlations in the dataset and similarities between cultivars, without dimensionality reduction. Results showed that anthocyanins, particularly pelargonidin-3-O-rutinoside, cyanidin-3,5-diglucoside and cyanidin-3-O-rutinoside, were the main contributors to the peels’ free radical scavenging capacity. This capacity was particularly higher in the peel of dark-colored figs compared to the fruit pulp. The local cultivar “INRA 1301” showed the most promising phenolic profile due to its very high levels of almost all detected PCs, especially (−)-epicatechin, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, cyanidine-3,5-diglucoside, cyanidine-3-O-rutinoside and pelargonidin-3-O-rutinoside (54.66, 141.08, 35.48, 494.08, 478.66, 12.56 µg/g dw, respectively). Having the darkest figs in the collection (L* = 25.72, c* = 22.09 and h° = 20.99), this cultivar has also combined promising IC50 values, which were of 19.85, 40.58 and 124.78 µg/mL for DPPH, ABTS and LPIC essays, respectively.     

Kinetic,isotherm, and mechanism investigations of the removal of nitrate and nitrite from water by the synthesized hydrotalcite Mg–Al

Research on Chemical Intermediates - The objective of this work is to test the effectiveness of hydrotalcite as an adsorbent for the retention of nitrate and nitrite ions and to study the influence...