Iridoid Sex Pheromone Biosynthesis in Aphids Mimics Iridoid-Producing Plants

Biosynthesis of (1R,4aS,7S,7aR)-nepetalactol ( 1 ) and (4aS,7S,7aR)-nepetalactone ( 2 ) in plants involves iridoid synthase (ISY), an atypical reductive cyclase that catalyses the reduction of 8-oxogeranial into the reactive enol of (S)-8-oxocitronellal, and cyclization of this enol intermediate, either non-enzymatically or by a nepetalactol-related short chain dehydrogenase enzyme (NEPS) that yields the nepetalactols. In this study, we investigated the biosynthesis in vivo of 1 and 2 in the pea aphid, Acyrthosiphon pisum, using a library of isotopically-labelled monoterpenoids as molecular probes. Topical application of deuterium-labelled probes synthesized from geraniol and nerol resulted in production of ²H₄−lactol 1 and ²H₄−lactone 2 . However, deuterium incorporation was not evident using labelled probes synthesized from (S)-citronellol. These results suggest that iridoid biosynthesis in animals, specifically aphids, may follow a broadly similar route to that characterised for plants.     

A parametric investigation of the performance of T-profiled highway noise barriers and the identification of a potential predictive approach

Although a considerable amount of research has been undertaken regarding the performance of T-profile noise barriers, the information available to the practicing highway engineer is confusing. For example, there is a widespread belief that the performance of a top edge, expressed as an insertion loss relative to that of the simple barrier on which it is mounted, is constant, irrespective of the relative locations of the source, barrier and receiver. In order to clarify the situation an investigation has been undertaken, using computer modelling, of the performance afforded by highway noise barriers with T-profile tops with different acoustic treatments. The relative insertion loss was found to increase systematically with increasing top width. Although the relative insertion loss afforded by a reflective T-top is small, significant attenuation can be obtained with an absorptive top. Examination of the effect on performance of the locations of source and receiver relative to that of the noise barrier indicated that, for source and receiver locations typical of those experienced for highway noise barriers, the relative insertion loss for a given width of T-top was a function of (a) the path difference between sound travelling to the receiver via the barrier top and direct sound from the source to the receiver and (b) the barrier height. Plots of relative insertion loss versus the path difference, normalised with respect to barrier heights, for a range of T-top widths and absorbent treatment, resulted in a collapse of data around well defined trend lines which offer the potential of being developed into a prediction method.     

Fiber containment for improved laboratory handling and uniform nanocoating of milligram quantities of carbon nanotubes by atomic layer deposition

The presence of nanostructured materials in the workplace is bringing attention to the importance of safe practices for nanomaterial handling. We explored novel fiber containment methods to improve the handling of carbon nanotube (CNT) powders in the laboratory while simultaneously allowing highly uniform and controlled atomic layer deposition (ALD) coatings on the nanotubes, down to less than 4 nm on some CNT materials. Moreover, the procedure yields uniform coatings on milligram quantities of nanotubes using a conventional viscous flow reactor system, circumventing the need for specialized fluidized bed or rotary ALD reactors for laboratory-scale studies. We explored both fiber bundles and fiber baskets as possible containment methods and conclude that the baskets are more suitable for coating studies. An extended precursor and reactant dose and soak periods allowed the gases to diffuse through the fiber containment, and the ALD coating thickness scaled linearly with the number of ALD cycles. The extended dose period produced thicker coatings compared to typical doses on CNT controls not encased in the fibers, suggesting some effects due to the extended reactant dose. Film growth was compared on a range of single-walled NTs, double-walled NTs, and acid-functionalized multiwalled NTs, and we found that ultrathin coatings were most readily controlled on the multiwalled NTs.