IR, UV-Vis, magnetic and thermal characterization of chelates of some catecholamines and 4-aminoantipyrine with Fe(III) and Cu(II)

The dopamine derivatives participate in the regulation of wide variety of physiological functions in the human body and in medication life. Increase and/or decrease in the concentration of dopamine in human body reflect an indication for diseases such as Schizophrenia and/or Parkinson diseases. Alpha-methyldopa (alpha-MD) in tablets is used in medication of hypertension. The Fe(III) and Cu(II) chelates with coupled products of adrenaline hydrogen tartarate (AHT), levodopa (LD), alpha-MD and carbidopa (CD) with 4-aminoantipyrine (4-AAP) are prepared and characterized. Different physico-chemical methods like IR, magnetic and UV-Vis spectra are used to investigate the structure of these chelates. Fe(III) form 1:2 (M:catecholamines) chelates while Cu(II) form 1:1 chelates. Catecholamines behave as a bidentate mono- or dibasic ligands in binding to the metal ions. IR spectra show that the catecholamines are coordinated to the metal ions in a bidentate manner with O,O donor sites of the phenolic -OH. Magnetic moment measurements reveal the presence of Fe(III) chelates in octahedral geometry while the Cu(II) chelates are square planar. The thermal decomposition of Fe(III) and Cu(II) complexes is studied using thermogravimetric (TGA) and differential thermal analysis (DTA) techniques. The water molecules are removed in the first step followed immediately by decomposition of the ligand molecules. The activation thermodynamic parameters, such as, energy of activation, enthalpy, entropy and free energy change of the complexes are evaluated and the relative thermal stability of the complexes are discussed.     

Effect of flash boiling injection on combustion and PN emissions of DISI optical engine fueled with butanol isomers/TPRF blends

Direct injection spark ignition (DISI) engines have been widely used in passenger cars due to their lower fuel consumption, better controllability, and high efficiency. However, DISI engines are suffering from wall wetting, imperfect mixture formation, excess soot emissions, and cyclic variations. Applying a new fuel atomization technique and using biofuels with their distinctive properties can potentially aid in improving DISI engines. In this research, the effects of isobutanol and 2-butanol and their blends with Toluene Primary Reference Fuel (TPRF) on spray characteristics, DISI engine combustion, and particle number (PN) emissions are investigated for conditions with and without flash boiling of the injected fuel. Spray characteristics are investigated using a constant volume chamber. Then, the combustion, flame propagation, and PN emissions are examined using an optical DISI engine. The fuel temperature is set to 298 K and 453 K for liquid injection and flash boiling injection, respectively. The tested blending ratio is 30 vol% butanol isomers and 70 vol% TPRF. The results of the spray test reveal that liquid fuel plumes are distinctly observed, and butanol blends show a slightly wider spray angle with lower penetration length compared to TPRF. However, under flash boiling injection, the sprays collapse towards the injector axis, forming a more extended single central vapor jet due to the plumes' interaction. Meanwhile, butanol blends yield a narrow spray angle with more extended penetration compared to TPRF. The flame visualization test shows that the flash boiling injection reduced yellow flames compared to liquid fuel injection, reflecting the improvements in mixture formation. Thus, improvements were noted in the heat release and PN emissions. Butanol addition reduced the PN emissions by 43% under regular liquid injection. Flash boiling injection provided an additional 25% reduction in PN emissions.     

Gene Expression Analysis in MCF-7 Breast Cancer Cells Treated with Recombinant Bromelain

The contributing molecular pathways underlying the pathogenesis of breast cancer need to be better characterized. The principle of our study was to better understand the genetic mechanism of oncogenesis for human breast cancer and to discover new possible tumor markers for use in clinical practice. We used complimentary DNA (cDNA) microarrays to compare gene expression profiles of treated Michigan Cancer Foundation-7 (MCF-7) with recombinant bromelain and untreated MCF-7. SpringGene analysis was carried out of differential expression followed by Ingenuity Pathway Analysis (IPA), to understand the underlying consequence in developing disease and disorders. We identified 1,102 known genes differentially expressed to a significant degree (p?相似文献   

Use of a Bacterially Expressed Human Factor IX Light Chain to Develop Polyclonal Antibody Anti-hFIX

Hemophilia B is an X-linked recessive bleeding disorder caused by deficiency or malfunctioning of human coagulation factor IX (hFIX). Hemophilia B patients are treated at present by infusion of plasma derived hFIX which is not always efficient, because development of anti-hFIX antibodies (alloantibodies) in some cases inhibits the activity of the infused hFIX. The hFIX alloantibodies are directed against γ-carboxyglutamic acid residues (Gla-domain) or protease domain in hFIX light chain. An epitope-containing fragment of hFIX light-chain was expressed in a T7-based Escherichia coli expression system and after purification, it was used for the immunization of rabbit to develop specific antibodies anti-hFIX. The plasma, derived from the immunized rabbit, was shown to be able to detect the normal hFIX, which indicates for the presence of a specific anti-hFIX antibody and supporting that a bacterially expressed hFIX subfragment might be able to neutralize the alloantibodies. Considering the importance of hFIX and its related investigations, both the produced hFIX antigen and its corresponding antibody will play important roles for experiments dealing with the production of hFIX and studies involved in the neutralization of the hFIX inhibitors in hFIX-related disorders and other clinical applications.     

Some remarks on global existence to the Cauchy problem of the wave equation with nonlinear dissipation

In this paper we prove the existence of global decaying H² solutions to the Cauchy problem for a wave equation with a nonlinear dissipative term by constructing a stable set in H¹(?ⁿ ). (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

Investigation of radioactivity in selected drinking water samples from Maryland

The comparator instrumental neutron activation analysis (INAA) method was used for certification of arsenic in Korea Research Institute of Standards and Science (KRISS) reference materials 108-04-001 (Oyster Tissue). The certified value was determined by analyzing one sample of approximately 0.15 g from ten bottles selected by random sampling. Potentially significant sources of uncertainty were taken into consideration using the guideline given by ISO. A complete evaluation of all sources of uncertainty yielded an expanded uncertainty for the mean value of this reference material of 4.7% at approximately 95% level of confidence.     

Sur le groupe des classes d'un anneau intègre

In this paper, we are interested in a generalization of Nagata's theorem [13] to a new class of domains other than Krull domains, the Mori domains and the PVMD domains (Theorem 1).      

The Euler–Bernoulli beam equation with boundary dissipation of fractional derivative type

We consider a Euler–Bernoulli beam equation with a boundary control condition of fractional derivative type. We study stability of the system using the semigroup theory of linear operators and a result obtained by Borichev and Tomilov. Copyright © 2016 John Wiley & Sons, Ltd.     

Spectrophotometric determination of flucloxacillin and dicloxacillin in pure and dosage forms

A simple, rapid and accurate spectrophotometric method for the determination of antibiotic drugs, flucloxacillin (Fluclox) and dicloxacillin (Diclox), in pure form and different pharmaceutical preparations has been developed. The charge transfer (CT) reactions between Fluclox and Diclox as electron donors and 7,7,8,8-tetracyanoquinodimethane (TCNQ) and tetracyanoethylene (TCNE) as pi-acceptors to give highly coloured complex species have been spectrophotometrically studied. The optimum experimental conditions for these CT reactions have been studied carefully. Beer's law is obeyed over the concentration ranges of 4-180 microg mL(-1) and 4-70 microg mL(-1) for Fluclox and Diclox drugs using TCNQ and TCNE reagents, respectively. The Sandell sensitivities (S) are found to be 0.016-0.035 microg cm(-2) and 0.011-0.016 microg cm(-2) for Fluclox and Diclox, respectively, which indicate the high sensitivity of the proposed method. The relative standard deviations (R.S.D.: 0.08-0.49 and 0.15-0.80) for the determination of Fluclox and (R.S.D.: 0.05-0.75 and 0.13-0.75) for Diclox were obtained for four to six replicates using TCNQ and TCNE reagents, respectively, refer to the high accuracy and precision of the proposed method. These results are also confirmed by the between-day precision and the percent recovery of 99.90-100.1 and 99.60-100.4 for Fluclox and 99.90-100.5 and 99.40-100.1 for Diclox using TNCQ and TCNE reagents, respectively. The results obtained for the two reagents are comparable with those obtained by the official method.