MAGfect: a novel liposome formulation for MRI labelling and visualization of cells

Cellular entry of imaging probes, such as contrast agents for magnetic resonance imaging (MRI), is a key requirement for many molecular imaging studies, particularly imaging intracellular events and cell tracking. Here, we describe the successful development and in vitro analysis of MAGfect, a novel liposome formulation containing a lipidic gadolinium contrast agent for MRI, Gd-DOTA-Chol , designed to enter and label cells. Liposome formulation and cell incubation time were optimised for maximum cellular uptake of the imaging probe in a variety of cell lines. MRI analysis of cells incubated with MAGfect showed them to be highly MRI active. This formulation was examined further for cytotoxicity, cell viability and mechanism of cell labelling. One of the key advantages of using MAGfect as a labelling vehicle arises from its potential for additional functions, such as concomitant drug or gene delivery and fluorescent labelling. The gadolinium liposome was found to be an effective vehicle for transport of plasmid DNA (pDNA) into cells and expression levels were comparable to the commercial transfection agent Trojene.     

The multiplicity of solutions in non-homogeneous boundary value problems

We use a method recently devised by Bolle to establish the existence of an infinite number of solutions for various non-homogeneous boundary value problems. In particular, we consider second order systems, Hamiltonian systems as well as semi-linear partial differential equations. The non-homogeneity can originate in the equation but also from the boundary conditions. The results are more satisfactory than those obtained by the standard “Perturbation from Symmetry” method that was developed – in various forms – in the early eighties by Bahri–Berestycki, Struwe and Rabinowitz. Received: 13 August 1998 / Revised version: 6 July 1999     

Buckling of elastic columns with second-mode imperfections

Meccanica - The pin-ended, slender, Euler strut has been used as the archetypal buckling problem for many years (Euler in Additamentum I de curvis elasticis, methodus inveniendi lineas curvas...     

Palladium(II) Immobilized Onto the Glucose Functionalized Magnetic Nanoparticle as a New and Efficient Catalyst for the One‐pot Synthesis of Benzoxazoles

Palladium(II) have been immobilized into the nano magnetic Fe₃O₄ which was functionalized with glucose in order to achieve a one‐pot synthesis of 2‐substituted benzoxazole derivatives with high yields in the diverse range of organic solvents. The nano catalyst is highly dispersive in polar solvents and can be easily recovered and reused for 6 runs without significant loss of its activity. Finally, the catalyst was fully characterized by FT‐IR, TGA, CHN, SEM, EDX and atomic absorption spectroscopy.     

Beyond Chemoselectivity: Catalytic Site‐Selective Aldolization of Diketones and Exploitation for Enantioselective Alzheimer's Drug Candidate Synthesis

Site selectivity, differentiating instances of the same functional group type on one substrate, represents a forward‐looking theme within chemistry: reduced dependence on protection/deprotection protocols for increased overall yield and step‐efficiency. Despite these potential benefits and the expanded tactical advantages afforded to synthetic design, site selectivity remains elusive and especially so for ketone‐based substrates. Herein, site‐selective intermolecular mono‐aldolization has been demonstrated for an array of prochiral 4‐keto‐substituted cyclohexanones with concomitant regio‐, diastereo‐, and enantiocontrol. Importantly, the aldol products allow rapid access to molecularly complex ketolactones or keto‐1,3‐diols, respectively containing three and four stereogenic centers. The reaction conditions are of immediate practical value and general enough to be applicable to other reaction types. These findings are applied in the first enantioselective, formal, synthesis of a leading Alzheimer's research drug, a γ‐secretase modulator (GSM), in the highest known yield.     

Shock Wave Pressure and Velocity Measuring Using a Novel Optic Sensor in a Newly Designed Diaphragm-less Shock Tube

Experimental Techniques - This paper suggests a new optical system for measuring shock wave characteristic that is installed in a shock tube. This shock tube has an innovative pneumatic rapid...     

Dynamic fluid flow behaviour of a tank draining through a vertical tube

This paper describes the unsteady draining of a sealed tank partially filled with water. The water discharges via a vertical tube into an open tank at atmospheric conditions. The air inflow, compensating for the volume of the discharged liquid, enters the system in an oscillatory manner, much like the “gulping” seen in an upended beer bottle. A mathematical model, based closly on that derived by Dougall & Kathiresan [Chem. Engng Commun. 8, 289–304 (1981)], has been applied to predict the pressure fluctuations in the closed tank. The rate of water discharge from the tank has been predicted and gives a much closer agreement with experimental results than a prediction based on a steady counter-current flooding limitation approach. A drift flux model has been used to describe the two-phase flow effect in the tube and the Wallis flooding criterion has been modified for use in the slug flow regime to describe the boundary conditions at the bottom of the tube. The pressure fluctuations in the sealed tank have been measured and compared with results obtained from the mathematical prediction for a variety of tube diameters.     

An Efficient and Clean Synthesis of Symmetrical and Unsymmetrical 3,3-Di(indolyl)Indolin-2-ones Using KSF

A facile and convenient protocol was developed for the synthesis of symmetrical and unsymmetrical 3,3-di(indolyl)indolin-2-ones in low reaction times (22–35 min) and high yields (85–95%) using KSF as an efficient recyclable heterogeneous catalyst.     

Synthesis and Characterization of Zinc Oxide Nano-particles by Solid State Chemical Reaction Method

Several important synthetic parameters such as precursor concentration, reaction time are found to determine the growth of ZnO nanostructures. These reaction parameters can be tuned to produce a variety size of nanostructures. In this work we show the importance of these parameters on the size of synthesized zinc oxide nano-powders. ZnO nanoparticles are synthesized by the solid-state reaction using ZnSO₄·7H₂O and NaOH as the reagents. In this method Zn(OH)₂ is the intermediate product of the reaction, we show that by adjusting the molar ratio of the reagents and grinding time, we can be removed this unwanted component in the final product so for obtaining pure ZnO nanostructures the calcinations process is not necessary, also we can tune the size of ZnO nanoparticles. XRD spectra of the nanoparticles demonstrate typical diffraction peaks of a well-crystalline Wurtzite ZnO structure transmission electron microscopic observations show that these nanoparticles are of hexagonal phase ZnO mostly in round shapes and he composition analysis by EDX indicate that final product is pure ZnO. In the optimum conditions by XRD analysis we see that the mean grain size of synthesized zinc oxide nano-particles is about 44 nm.