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排序方式: 共有135条查询结果,搜索用时 15 毫秒
81.
Knall AC Tscherner M Noormofidi N Pein A Saf R Mereiter K Ribitsch V Stelzer F Slugovc C 《The Analyst》2012,137(3):563-566
The nonradiative deactivation of the excited state of europium(III)-based phosphors via OH-vibrations in the presence of water allows the detection of moisture in nitrogen at concentration levels down to at least 25 ppm (0.09% RH). 相似文献
82.
Thomas Endres Olivia Merkel Moritz Beck‐Broichsitter Nadja Bege Holger Petersen Catherine Curdy Thomas Kissel 《Macromolecular bioscience》2012,12(7):970-978
The aim of this study is to investigate the feasibility and efficacy of PEC nanoparticles as delivery system for cancer chemotherapy. Assembly of paclitaxel‐loaded nanoparticles with high loading efficiency and narrow‐size distribution is successful. For non‐invasive in vivo tracing, nanoparticle blends of chelator bearing poly(lactide) with PEC and PLGA are successfully prepared. Pharmacokinetic studies in mice reveal a twofold higher circulation time of PEC as compared to PLGA. A tumor model shows an accumulation of PEC NPs in cancerous tissue and a higher anti‐tumor efficiency compared to the standard Taxol?, which is reflected in a significantly slower tumor growth compared to the NaCl control group.
83.
Nadja A. Simeth Shotaro Kobayashi Piermichele Kobauri Stefano Crespi Wiktor Szymanski Kazuhiko Nakatani Chikara Dohno Ben L. Feringa 《Chemical science》2021,12(26):9207
Short, complementary DNA single strands with mismatched base pairs cannot undergo spontaneous formation of duplex DNA (dsDNA). Mismatch binding ligands (MBLs) can compensate this effect, inducing the formation of the double helix and thereby acting as a molecular glue. Here, we present the rational design of photoswitchable MBLs that allow for reversible dsDNA assembly by light. Careful choice of the azobenzene core structure results in excellent band separation of the E and Z isomers of the involved chromophores. This effect allows for efficient use of light as an external control element for duplex DNA formation and for an in-depth study of the DNA–ligand interaction by UV-Vis, SPR, and CD spectroscopy, revealing a tight mutual interaction and complementarity between the photoswitchable ligand and the mismatched DNA. We also show that the configuration of the switch reversibly dictates the conformation of the DNA strands, while the dsDNA serves as a chiral clamp and translates its chiral information onto the ligand inducing a preference in helical chirality of the Z isomer of the MBLs.We present the rational design of photoswitchable DNA glue to trigger the reversible formation of duplex DNA by light. The supramolecular assembly shows a mutual interaction between ligand and DNA, which induces a preferred helicity in the switch. 相似文献
84.
Lopez-Garcia LA Schulze JO Fröhner W Zhang H Süss E Weber N Navratil J Amon S Hindie V Zeuzem S Jørgensen TJ Alzari PM Neimanis S Engel M Biondi RM 《Chemistry & biology》2011,18(11):1463-1473
Protein kinases are key mediators of cellular signaling, and therefore, their activities are tightly controlled. AGC kinases are regulated by phosphorylation and by N- and C-terminal regions. Here, we studied the molecular mechanism of inhibition of atypical PKCζ and found that the inhibition by the N-terminal region cannot be explained by a simple pseudosubstrate inhibitory mechanism. Notably, we found that the C1 domain allosterically inhibits PKCζ activity and verified an allosteric communication between the PIF-pocket of atypical PKCs and the binding site of the C1 domain. Finally, we developed low-molecular-weight compounds that bind to the PIF-pocket and allosterically inhibit PKCζ activity. This work establishes a central role for the PIF-pocket on the regulation of PKCζ and allows us to envisage development of drugs targeting the PIF-pocket that can either activate or inhibit AGC kinases. 相似文献
85.
Lübcke A Buchner F Heine N Hertel IV Schultz T 《Physical chemistry chemical physics : PCCP》2010,12(43):14629-14634
Time-resolved photoelectron spectroscopy was used to study the energetics and dynamics of solvated electrons in aqueous solution. Solvated electrons are generated by ultrafast photodetachment in a 100 mM aqueous NaI solution. Initially, an ensemble of strongly bound ("cold") solvated electrons and an ensemble of weakly bound ("hot") electrons in an unequilibrated solvent environment are observed. We report an ultrafast recombination channel for the "hot" electrons with a rate of (800 fs)(-1) which is in competition with thermalization occurring with a rate of (1.1 ps)(-1). The thermalized electrons recombine with the iodide radical with a rate of (22 ps)(-1). About 35% of the thermalized electrons escape geminate recombination and form free, solvated electrons. The vertical detachment energy for the solvated electron is determined to be 3.40 eV. No indication for a surface-bound electron at lower binding energies was observed. 相似文献
86.
Josep L. Acero Sanchez Olivier Y. F. Henry Teresa Mairal Nadja Laddach Anders Nygren Siegfried Hauch Jasmin Fetisch Ciara K. O’Sullivan 《Analytical and bioanalytical chemistry》2010,397(6):2325-2334
An enzyme-linked oligonucleotide assay (ELONA) for quantification of mRNA expression of five genes involved in breast cancer,
extracted from isolated rare tumour cells and amplified by multiplex ligation-dependent probe amplification (MLPA) is presented.
In MLPA, a multiplex oligonucleotide ligation assay is combined with a PCR reaction in which all ligation products are amplified
by use of a single primer pair. Biotinylated probes complementary to each of the target sequences were immobilised on the
surface of a streptavidin-coated microtitre plate and exposed to single-stranded MLPA products. A universal reporting probe
sequence modified with horseradish peroxidase (URP–HRP) and complementary to a universal primer used during the MLPA step
was further added to the surface-bound duplex as a reporter probe. Simultaneous addition of anchoring probe and target, followed
by addition of reporter probe, rather than sequential addition, was achieved with no significant effect on sensitivity and
limits of detection, but considerably reduced the required assay time. Detection limits as low as 20 pmol L−1, with an overall assay time of 95 min could be achieved with negligible cross-reactivity between probes and non-specific
targets present in the MLPA-PCR product. The same MLPA-PCR product was analysed using capillary electrophoresis, the technique
typically used for analysis of MLPA products, and good correlation was observed. The assay presented is easy to carry out,
relatively inexpensive, rapid, does not require sophisticated instrumentation, and enables quantitative analysis, making it
very promising for the analysis of MLPA products. 相似文献
87.
Nadja Noormofidi 《European Polymer Journal》2010,46(4):694-701
Two norbornene derivatives bearing phenantroimidazole dyes, namely endo/exo-11-(4-(1H-phenanthro[9,10-d]imidazol-2-yl)phenoxy) undecylbicyclo[2.2.1] hept-5-ene-2-carboxylat (5) and 11-(4-(1-Methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenoxy) undecylbicyclo[2.2.1]hept-5-ene-2-carboxylate (6) were synthesized. 5 and 6 were used to prepare statistical copolymers with endo,exo-bicyclo[2.2.1.]hept-2-ene-5,6-dicarboxylic acid dimethylester (7) via ring opening metathesis polymerization. The photophysical properties of the monomers and polymers and to what extent polymerization and substitution patterns influence the photophysical properties were investigated. Furthermore, the optical response upon addition of acid was investigated. The emission of monomer 5 shows a small bathochromic shift of 13 nm upon protonation while the emission of the resultant polymer poly5/7 exhibits a large red shift of 62 nm. In contrast, the according methylated monomer 6 and the resulting polymer poly6/7 gave similar absorption and emission characteristics. The differences were attributed to an increased tendency of the non-methylated dyes to interact when brought in close proximity in the polymer. 相似文献
88.
Conformation Diversity of a Fused‐Ring Pyrazine Derivative on Au(111) and Highly Ordered Pyrolytic Graphite 下载免费PDF全文
Hui‐Juan Yan Nadja Sändig Haifeng Wang Dong Wang Francesco Zerbetto Xiaowei Zhan Li‐Jun Wan 《化学:亚洲杂志》2015,10(6):1311-1317
Heterocyclic aromatic compounds have attracted considerable attention because of their high carrier mobility that can be exploited in organic field‐effect transistors. This contribution presents a comparative study of the packing structure of 3,6‐didodecyl‐12‐(3,6‐didodecylphenanthro[9,10‐b]phenazin‐13‐yl)phenanthro[9,10‐b]phenazine (DP), an N‐heterocyclic aromatic compound, on Au(111) and highly ordered pyrolytic graphite (HOPG). High‐resolution scanning tunneling microscopy (STM) combined with atomistic simulations provide a picture of the interface of this organic semiconductor on an electrode that can have an impact on the field‐effect transistor (FET) performance. DP molecules adsorb with different conformational isomers (R/S: trans isomers; C: cis isomer) on HOPG and Au(111) substrates. All three isomers are found in the long‐range disordered lamella domains on Au(111). In contrast, only the R/S trans isomers self‐assemble into stable chiral domains on the HOPG surface. The substrate‐dependent adsorption configuration selectivity is supported by theoretical calculations. The van der Waals interaction between the molecules and the substrate dominates the adsorption binding energy of the DP molecules on the solid surface. The results provide molecular evidence of the interface structures of organic semiconductors on electrode surfaces. 相似文献
89.
90.
Nadja Frömmgen Dimiter L. Balabanski Mark L. Bissell Jacek Bieroń Klaus Blaum Bradley Cheal Kieran Flanagan Stephan Fritzsche Christopher Geppert Michael Hammen Magdalena Kowalska Kim Kreim Andreas Krieger Rainer Neugart Gerda Neyens Mustafa M. Rajabali Wilfried Nörtershäuser Jasna Papuga Deyan T. Yordanov 《The European Physical Journal D - Atomic, Molecular, Optical and Plasma Physics》2015,69(6):164