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Nanotube molecular transporters: internalization of carbon nanotube-protein conjugates into Mammalian cells 总被引:20,自引:0,他引:20
Shi Kam NW Jessop TC Wender PA Dai H 《Journal of the American Chemical Society》2004,126(22):6850-6851
The interactions between various functionalized carbon nanotubes and several types of human cancer cells are explored. We have prepared modified nanotubes and have shown that these can be derivatized in a way that enables attachment of small molecules and of proteins, the latter through a novel noncovalent association. The functionalized carbon nanotubes enter nonadherent human cancer cells as well as adherent cell lines (CHO and 3T3) and by themselves are not toxic. While the fluoresceinated protein streptavidin (MW approximately 60 kD) by itself does not enter cells, it readily enters cells when complexed to a nanotube-biotin transporter and exhibits dose-dependent cytotoxicity. The uptake pathway is consistent with adsorption-mediated endocytosis. The use of carbon nanotubes as molecular transporters could be exploited for various cargos. The biocompatibility and unique physical, electrical, optical, and mechanical properties of nanotubes provide the basis for new classes of materials for drug, protein, and gene delivery applications. 相似文献
24.
Cruz-Aguado JA Chen Y Zhang Z Elowe NH Brook MA Brennan JD 《Journal of the American Chemical Society》2004,126(22):6878-6879
Firefly luciferase (FL) was entrapped in sol-gel-derived silica containing precursors based on covalent linkage of d-gluconolactone or d-maltonolactone to (aminopropyl)triethoxysilane to form N-(3-triethoxysilylpropyl)gluconamide or N-(3-triethoxysilylpropyl)maltonamide. The enzyme was active and stable in this material and showed catalytic constants close to those in solution. As little as 20 amol ATP could be detected with the entrapped FL, and the entrapped enzyme could be used over several cycles. 相似文献
25.
Marco Di Summa 《Operations Research Letters》2008,36(1):7-13
For the problem of lot-sizing on a tree with constant capacities, or stochastic lot-sizing with a scenario tree, we present various reformulations based on mixing sets. We also show how earlier results for uncapacitated problems involving (Q,SQ) inequalities can be simplified and extended. Finally some limited computational results are presented. 相似文献
26.
The purpose of this work is to describe an abstract theory of Hardy-Sobolev spaces on doubling Riemannian manifolds via an atomic decomposition. We study the real interpolation of these spaces with Sobolev spaces and finally give applications to Riesz inequalities. 相似文献
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Abderrahim A?t Adoubel Christophe J. Morin Nadine Mofaddel Georges Dupas Paul-Louis Desbène 《Analytical and bioanalytical chemistry》2009,394(2):597-608
Enantiomer separations of underivatised amino acids were carried out by using ligand exchange capillary electrophoresis (LECE).
Chiral discrimination is based on the formation of ternary complexes between copper(II), a chiral selector (L-proline or trans-4-hydroxy-L-proline) and an amino acid. All amino acids containing aromatic moieties or not were detected at 214 nm because
of their interactions with copper(II). In order to reduce copper(II) adsorption onto capillary walls, we used hexadimethrine
bromide to reverse the electroosmotic flow. Using this original strategy, the studied enantiomers migrated in the opposite
direction of the anodic electroosmosis. After optimising the analytical conditions taking into account the chiral resolution
and the detection sensitivity, we performed very satisfactory enantioseparations not only of aromatic amino acids (tryptophan,
tyrosine, phenylalanine and histidine) but also of aliphatic amino acids (threonine, serine, isoleucine and valine). These
enantioseparations were performed in a short analysis time at 35 °C. In order to rationalise the obtained results, we evaluated
the complexation constants corresponding to the formed ternary complexes by capillary electrophoresis and we used molecular
mechanics modelling. 相似文献
29.
Muhammad Nawaz Tahir Markus Messerschmidt Michael Klein Victor Martinez Patrick Theato Nadine Metz Sonngard Hartmann Ute Kolb Vadim Ksenofontov Franz Renz Wolfgang Tremel 《Polyhedron》2009,28(9-10):1728-1733
The precursor [FeIII(L)Cl (L = N,N′-bis(2′-hydroxy-3′-methyl-benzyliden)-1,7-diamino-4-azaheptane) is combined with [Mo(CN)8]4? yields a star shaped nona-nuclear cluster, [MoIV{(CN)FeIII(L)}8]Cl4. This Fe8Mo molecule is a high-spin system at room temperature. On cooling to 20 K some of the iron(III) centres in the molybdenum(IV)-star switch to the low-spin state as proven by Mössbauer spectroscopy. This molecule was deposited on TiO2 nanowires by electrostatic interactions between the cluster cations and the surface functionalized titanium oxide nanowire. The synthesis and surface binding of the multistable molecular switch was demonstrated using IR and UV–Vis spectroscopy (high-resolution) transmission electron microscopy ((HR)TEM) and Mössbauer spectroscopy. High- and low-temperature Mössbauer spectra indicate that the spin state transition of the free cluster molecules is preserved after surface binding. The above results emphasize the possibility of fabricating molecule-based low-dimensional structures by using traditional bottom-up approaches based on the electrostatic interaction between the cluster cations and polymer functionalized nanowires. These results can be generalized for the application to both charged and non-charged molecules. 相似文献
30.
Nadine Werth Mathias S. Müller Johann Meier Alexander W. Koch 《Optics Communications》2011,284(9):2317-2322
Using Laser-based Speckle-Interferometers, the shape of optically rough surfaces can be measured precisely and contactlessly from variable measuring distances even in regions of difficult access. This work is concerned with the integration of a micromirror array (MMA) into an electronic Speckle-Pattern-Interferometer. With the adaptive optics, it is intended to adapt the phasefront of a reference wave to critical surface areas of the measurement object. Yet, due to the topography of the MMA, diffraction effects occur which affect the phase and intensity of the generated wavefront. We demonstrate how these diffraction effects can be efficiently modelled by a Fraunhofer diffraction method. We compare the results of this model to theoretical data obtained by a numerical Fresnel diffraction model and to measurement data obtained from a measurement setup incorporating a multi mirror array. 相似文献