A new high performance liquid chromatographic method for the simultaneous measurement of S-adenosylmethionine and S-adenosylhomocysteine. Concentrations in pig tissues after inactivation of methionine synthase by nitrous oxide

A rapid gradient method has been established to measure simultaneously the tissue levels of S-adenosylmethionine (AdoMet) and S-adenosylhomocysteine (AdoHcy). The method involves application of a 15%-25% linear gradient of methanol over a period of 10 min to an initial mobile phase of 15% methanol with 25 mM sodium phosphate, 10 mM 1-heptanesulfonic acid solution at pH 3.2 and a flow rate of 1 mL/min. AdoHcy elutes at 9.5 min and AdoMet at 12.6 min. The assay has a detection limit of 10 pmol and is linear in the concentration range 30-800 pmol. The method was used to assess changes in AdoMet and AdoHcy concentrations of pig tissues after seven days exposure to the anaesthetic gas nitrous oxide which irreversibly inactivates methionine synthase and induces an inability to recycle homocysteine, particularly in neural tissues. The treatment caused significant alterations in cellular AdoMet:AdoHcy ratios which were principally due to a dramatic rise in AdoHcy concentrations.     

Heat Output from Spreading and Rifting Models of the Taupo Volcanic Zone, New Zealand

A conceptual model of the Taupo Volcanic Zone (TVZ) is developed, to a depth of 25 km, formed from three constant density layers. The upper layer is formed from eruption products. A constant rate of eruption is assumed, which eventually implies a constant rate of extension, and a constant rate of volumetric creation in the middle and bottom layers. Tectonic extension creates volume which can accomodate magmatic intrusions. Spreading models assume this volume is distributed throughout the whole region, perhaps in vertical dykes, whereas rifting models assume the upper crust is thinned and the volume created lies under this upper crust. Bounds on the heat flow from such magmatic intrusions are calculated. Heat flow calculations are performed and some examples are provided which match the present total heat output from the TVZ of about 4200 MW, but these either have extension rates greater than the low values of about 8 ± 4 mm/a being reported from GPS measurements, or else consider extension rates in the TVZ to have varied over time.     

J/psi production in Indium-Indium collisions at 158 GeV/nucleon

The NA60 experiment studies muon pair production at the CERN Super Proton Synchrotron. In this Letter we report on a precision measurement of J/psi in In-In collisions. We have studied the J/psi centrality distribution, and we have compared it with the one expected if absorption in cold nuclear matter were the only active suppression mechanism. For collisions involving more than approximately 80 participant nucleons, we find that an extra suppression is present. This result is in qualitative agreement with previous Pb-Pb measurements by the NA50 experiment, but no theoretical explanation is presently able to coherently describe both results.     

Geometric properties of two phase flow in geothermal reservoirs

The two phase flow equations frequently used in geothermal engineering ignore capillary pressure, which results in a singular system of equations. Analysis of these equations reveals three mechanisms for altering saturation: local boiling, the spatial dependence of flowing enthalpy due to the convective transport of fluid, and counterflow. A scalar function is associated with each of these three mechanims. At each point in space, flows are essentially two dimensional, with gravity establishing a vertical hierarchy, in that volumetric, energy and mass fluxes can never point below a lower member in this triple. With increasing liquid saturation, the characteristics associated with the saturation equation move up from below this grouping of directions, and eventually may even point above volumetric fluxes. Finally, weak shocks and the associated entropy condition are considered. The characteristics of the saturation equation coincide with the velocity of extremely weak shocks, and saturation increases with the passage of a weak shock, provided the magnitude of the characteristic speed increases with saturation.Notation C_l liquid heat capacity - C_m rock heat capacity - C_v vapour heat capacity - G counterflow energy flux - h flowing enthalpy - hl liquid enthalpy - h_v vapour enthalpy - k permeability - k downward vector - P pressure - S liquid saturation - T temperature - dT/dP derivative at saturation - z vertical coordinate - l liquid viscosity - v vapour viscosity - Pl liquid density - _m rock density - _v vapour density - porosity     

Surface deposition from fluid flow in a porous medium

The changes to porosity and permeability resulting from surface deposition and early dissolution in an initial rhombohedral array of uniform spheres are studied. Very rapid decreases in permeability result from early deposition, with 48 percent reduction predicted in permeability from 8 percent reduction in porosity. After deposition has caused about a 1 percent increase in the radii of the spherical array, relative permeability reductions vary approximately as the square of relative changes in porosity. These theoretical results are matched with experimental data of Itoi et al. and Moore et al. on deposition of silica. Satisfactory results are obtained in some cases, but for other cases a more complex model of the porous medium is needed.     

Voltage-induced swelling and deswelling of weak polybase brushes

We have investigated a novel method of remotely switching the conformation of a weak polybase brush using an applied voltage. Surface-grafted polyelectrolyte brushes exhibit rich responsive behavior and show great promise as "smart surfaces", but existing switching methods involve physically or chemically changing the solution in contact with the brush. In this study, high grafting density poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) brushes were grown from silicon surfaces using atom transfer radical polymerization. Optical ellipsometry and neutron reflectivity were used to measure changes in the profiles of the brushes in response to DC voltages applied between the brush substrate and a parallel electrode some distance away in the surrounding liquid (water or D(2)O). Positive voltages were shown to cause swelling, while negative voltages in some cases caused deswelling. Neutron reflectometry experiments were carried out on the INTER reflectometer (ISIS, Rutherford Appleton Laboratory, UK) allowing time-resolved measurements of polymer brush structure. The PDMAEMA brushes were shown to have a polymer volume fraction profile described by a Gaussian-terminated parabola both in the equilibrium and in the partially swollen states. At very high positive voltages (in this study, positive bias means positive voltage to the brush-bearing substrate), the brush chains were shown to be stretched to an extent comparable to their contour length, before being physically removed from the interface. Voltage-induced swelling was shown to exhibit a wider range of brush swelling states in comparison to pH switching, with the additional advantages that the stimulus is remotely controlled and may be fully automated.     

HPLC-MS/MS法联合分析环境水体中喹诺酮类抗生素和雌激素

建立了环境水体中15种喹诺酮类抗生素和6种雌激素的HPLC-MS/MS分析方法.该方法统一了两类物质的前处理过程,运用固相萃取技术进行富集及净化,有效降低了基质的干扰.质谱采用SRM监测模式,喹诺酮采用正离子扫描模式,雌激素采用负离子扫描模式.目标化合物在水体中的回收率范围为62.0％～108.3％,RSD在1.1％ ...     

微波辐射法制备水溶性CdTe/ZnS量子点及其与司帕沙星的作用

以巯基乙酸为稳定剂,通过微波加热在水溶液中制备了CdTe/znS量子点.研究了pH值、反应时间、反应温度和cdTe/s<'2->浓度比等合成条件对cdTe量子点荧光光谱的影响.以CdTe/ZnS量子点为探针,探讨了喹诺酮类抗生素司帕沙星与量子点的荧光猝灭作用,结果表明,在最佳实验条件下,其线性范围为0.05～3.00μg/mL,线性相关系数为0.9954,检出限为0.01μg/mL,可将CdTe/ZnS量子点荧光探针用于司帕沙星的测定.     

缺位的Wells-Dawson型磷钨酸盐和Eu(Ⅲ)构筑的一维链状多金属氧酸盐化合物

在水溶液条件下以六缺位的[H2P2W12O48)]12-和Eu髥为反应前躯体得到了1个新的多金属氧酸盐化合物[Eu3(H2O)17(α2-HP2W17O61)].5H2O(1),对其进行了元素分析、红外光谱、热重、荧光等表征,并用X-射线单晶衍射测定了它的晶体结构。化合物1含有双支撑的多阴离子簇[{Eu(H2O)7}2{Eu(H2O)3(α2-P2W17)}2]8-,并进一步通过Eu髥连接形成了延c轴方向的一维链。室温条件下的荧光光谱研究表明:化合物1显示了强的红光发射。     

Effects of Hydroxyapatite Nanoparticles on Apoptosis and Invasion of Human Renal Cell Carcinoma 786-0 Cells

Renal cell carcinoma is the most common cancer of the kidney, and resistant to traditional therapies. The aim of this study is to investigate the effects of hydroxyapatite nanoparticles on human renal cell carcinoma 786-0 cells. Cell proliferation was assessed with an 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide(MTT) staining kit. The apoptosis assay was assessed with an FITC Annexin V Apoptosis Detection Kit. Caspase-3 and caspase-12 were detected by immunocytochemical staining and semi-qua...