Chemische synthese von nonadesoxyribonucleotiden mit den abgewandelten basen uracil, 5-bromuracil und 5-methylcytosin nach dem triester-verfahren

In order to investigate the interaction of Eco RII restriction and modification enzymes with synthetic DNA fragments three nonadeoxyribonucleotides containing the modified bases uracil, 5-bromouracil and 5-methylcytosine were synthesized according to the phosphate tri-ester approach using TPS/l-methylimidazole as the condensation agent. The patterns of these modified DNA fragments obtained by Maxam/Gilbert sequence technique are presented.     

Parametric analysis of the inductively coupled plasma

A systematic parametric study of an LTE (local thermodynamic equilibrium) mathematical model of pure inductively coupled argon plasma (ICAP) used for spectrochemical purposes was performed by means of computer simulations. The spatial distributions of temperature, gas velocity, magnetic field and energy losses were investigated under typical plasma operating conditions as function of the ICAP geometrical dimensions and dynamic parameters. These theoretical calculations can be used to predict the properties of the applied plasma in the course of practical work, to choose the optimal conditions by changing the operating parameters and to interpret existing analytical results.     

9,9-diphenyl-1,9,10,10a-tetrahydro-2H-phenaleno[1,9-bc]pyrans

Reaction between some naphtho[2,1-b]pyrans and 1,1-diphenylethene affords 9,9-diphenyl-1,9,10,10a-tetrahydro-2H-phenaleno[1,9-bc]pyrans.     

Optimization of performance and minimization of silicate interference in continuous flow phosphate analysis

Specific reaction conditions for automated continuous flow analysis of phosphate are optimized in regard to minimizing coating and silicate interference, while maintaining high sensitivity. Use of Sb in the reagent increases sensitivity and yields absorbances with little temperature dependence. Coating can be minimized by using a final solution at a pH>0.5. At final pH of 0.78 there is maximum interference from silicate in the sample. We recommend therefore as an optimal reaction condition with minimal silicate interference, the use of Sb, a final solution pH of 1.00, room temperature for the reaction and a [H(+)]/[Mo] ratio of 70. An equation is provided to correct silicate interference in high precision phosphate determination.     

The role of rigid unit modes in negative thermal expansion

The rigid unit mode (RUM) model provides a valuable computational method to investigate correlations of transverse thermal motions of atoms important in negative thermal expansion (NTE) materials. We report here detailed RUM calculations of ten framework oxide structures that have been studied for their negative (or ultra low) thermal expansion properties. The results negate any simple and direct correlation between presence or absence of RUMs in a structure and its NTE property. All the structures considered can be viewed as networks of polyhedral connected by corners only. All evidence supports the importance the transverse motion of the atoms at the corners, but NTE does not correlate well with the presence RUMs for the polyhedra.     

A potassium-selective electrode with solid internal contact

The performance characteristics of potassium-selective electrodes made with valinomycin membranes plasticized with dioctyl adipate or sebacate, and with a solid silver contact or an internal solution, have been critically examined. The choice of electrode depends on a number of factors, including the interfering ion(s) present. The electrodes can be used for determination of potassium in natural waters.     

Synthesis and some reactions of 3-cyanopyridine-2-thiones

New methods for the synthesis of 3-cyanopyridine-2-thiones by the reaction of -keto nitroles with sulfur and by condensation of chalcones or benzylideneacetone with cyanothioacetamide are given. The compounds obtained were used in various reactions for the preparation of alkylated products, disulfides, and condensed heterocycles, viz., thieno[2,3-b]pyridines and pyrido[2,3:2,3]thieno[4,5-d]-pyrimidines.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 3, pp. 377–382, March, 1981.     

5-[(p-Methyl-phenyl)azo]-8-aminoquinoline as a new reagent for trace determination of nickel by adsorptive cathodic stripping voltammetry

The synthesis of p-MPAQ and the electroanalytical chemical properties of the nickel(II)-p-MPAQ complex are reported in this paper. A sensitive adsorptive cathodic stripping procedure for trace nickel determination was developed. For a 3-min preconcentration time the detection limit is 3.0 × 10⁻¹¹M.     

Phage-display selection of a human single-chain fv antibody highly specific for melanoma and breast cancer cells using a chemoenzymatically synthesized G(M3)-carbohydrate antigen

Overexpression of the cell-surface glycosphingolipid G(M3) is associated with a number of different cancers, including those of the skin, colon, breast, and lung. Antibodies against the G(M3) epitope have potential application as therapeutic agents in the treatment of these cancers. We describe the chemoenzymatic synthesis of two G(M3)-derived reagents and their use in the panning of a phage-displayed human single-chain Fv (scFv) antibody library derived from the blood of cancer patients. Three scFv-phage clones, GM3A6, GM3A8, and GM3A15, were selected for recombinant expression and were characterized using BIAcore and flow cytometry. BIAcore measurements using the purified, soluble scFvs yielded dissociation constants (K(d)) ranging from 4.2 x 10(-7) to 2.1 x 10(-5) M. Flow cytometry was used to evaluate the ability of each scFv to discriminate between normal human cells (human dermal fibroblast, HDFa), melanoma cells (HMV-1, M21, and C-8161), and breast cancer cells (BCM-1, BCM-2, and BMS). GM3A6 displayed cross-reactivity with normal cells, as well as tumor cells, and GM3A15 possessed little or no binding activity toward any of the cell lines tested. However, GM3A8 bound to five of the six tumor cell lines and showed no measurable reactivity against the HDFa cells. Hence, we have demonstrated that a synthetic G(M3) panning reagent can be used to isolate a fully human scFv that is highly specific for native G(M3) on the surface of tumor cells. The result is a significant step toward effective immunotherapies for the treatment of cancer.     

Synthesis in the phenothiazine series

Quaternary salts of imidazo[4,5,1-k,l]phenothiazine were reduced with potassium borohydride to 1,2-dihydro-2-methylimidazo[4,5,1-k,l]phenothiazine, which was converted to 1,2 dihydro-2-methylimidazo[4,5,1-k,l]phenothiazine-1-thione and 1-methylamino-10-formylphenothiazine. The latter was hydrolyzed to 1-methylaminophenothiazine, which was also obtained by reduction of methyl phenothiazine-1-carbamate. The PMR and IR spectra of some of the derivatives are discussed.See [1] for communication XXXV.Translated from Khimiya Geterotsiklicheskikh Soedinenii, No. 3, pp. 372–374, March, 1974.