Multimodal approaches of the hydroalcoholic extract of Diospyros peregrina fruits in diabetic therapy

The matured fruits of Diospyros peregrina possess significant antidiabetic activity. The present investigation was undertaken to evaluate the target of action of the hydroalcoholic extract of D. peregrina fruit in diabetic pathophysiology. The extract was tested in vitro for inhibition of glucose absorption by α-amylase inhibition assay, glucose uptake study by isolated rat hemidiaphragm method and free radical scavenging activity by DPPH method. The extract exhibited significant α-amylase inhibition (IC?? 136.5 μg mL?1), peripheral glucose utilisation and radical scavenging (IC?? 167.5 μg mL?1) activity. From the results, it may be concluded that the hydroalcoholic extract of D. peregrina fruit can counteract diabetes by multiple mechanisms, namely inhibition of carbohydrate absorption, improving peripheral glucose utilisation and antioxidant defence.     

Chitosan nanoparticles of 5-fluorouracil for ophthalmic delivery: characterization, in-vitro and in-vivo study

The aim of this investigation was to develop 5-fluorouracil (5-FU) loaded chitosan nanoparticles (CH-DNPs) for ophthalmic delivery. CH-DNPs were fabricated by ionotropic gelation mechanism using chitosan (CH) and a polyanion (TPP). The nanoparticles were smooth and spherical, confirmed by scanning electron microscopy (SEM) and atomic force microscope (AFM). CH/TPP mass ratio and TPP significantly changed the particles size morphology and encapsulation efficiency. The nanoparticles size ranged from approximately 114 to 192 nm and had a positive zeta potential (30±4 mV). The encapsulation efficiency, loading capacity and recovery of DNPs were 8.12-34.32%, 3.14-15.24% and 24.22 to 67% respectively. Physical characterization was done by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD). No interaction was observed in between drug and polymer and crystallinity of drug was not changed in drug loaded nanoparticles. In-vitro release study of DNPs showed diffusion controlled release. Bioavailability study of batch CS9 was studied in rabbit eye and compare to 5-FU solution. 5-FU level was significantly higher in aqueous humor of rabbit eye. Ocular tolerance was studied in the eye of New Zealand rabbits and tested formulation was non-irritant with no sign of inflammation.     

Multiplex cancer cell detection by SERS nanotags with cyanine and triphenylmethine Raman reporters

SERS nanotags have been prepared to accomplish the multiplex detection of cancer cells. Herein we evaluated the adequacy of lipoic acid-containing cyanine derivatives (Cy3LA and Cy5LA) to function as multiplex partners with a triphenylmethine Raman reporter (B2LA) under a single excitation wavelength. SERS experiments enabled the multiplex recognition of two different cancer cells with antibody-conjugated nanotags that were derivatized with optimized cyanine and triphenylmethine reporters.     

Chemoselectivity in the Cu-catalyzed O-arylation of phenols and aliphatic alcohols

An orthogonal set of Cu-catalysts for the selective mono-arylation of alkyl aryl diols using aryl iodides is presented. Picolinic acid ligated copper catalyst provided phenol O-arylation only, while alkyl aryl ethers are generated by ligand-free copper catalyst in the presence of 2 equivalents NaOt-Bu.     

Two storage inventory model in a mixed environment

Multi-item inventory models with two storage facility and bulk release pattern are developed with linearly time dependent demand in a finite time horizon under crisp, stochastic and fuzzy-stochastic environments. Here different inventory parameters—holding costs, ordering costs, purchase costs, etc.—are assumed as probabilistic or fuzzy in nature. In particular cases stochastic and crisp models are derived. Models are formulated as profit maximization principle and three different approaches are proposed for solution. In the first approach, fuzzy extension principle is used to find membership function of the objective function and then it’s Graded Mean Integration Value (GMIV) for different optimistic levels are taken as equivalent stochastic objectives. Then the stochastic model is transformed to a constraint multi-objective programming problem using Stochastic Non-linear Programming (SNLP) technique. The multi-objective problems are transferred to single objective problems using Interactive Fuzzy Satisfising (IFS) technique. Finally, a Region Reducing Genetic Algorithm (RRGA) based on entropy has been developed and implemented to solve the single objective problems. In the second approach, the above GMIV (which is stochastic in nature) is optimized with some degree of probability and using SNLP technique model is transferred to an equivalent single objective crisp problem and solved using RRGA. In the third approach, objective function is optimized with some degree of possibility/necessity and following this approach model is transformed to an equivalent constrained stochastic programming problem. Then it is transformed to an equivalent single objective crisp problem using SNLP technique and solved via RRGA. The models are illustrated with some numerical examples and some sensitivity analyses have been presented.     

Inventory of differential items selling at two shops under single management

Inventory of differential (both good and defective) items purchased in a lot for two shops under a single management is considered here. In the primary shop, the lot is received and separated, only good units are sold and shortages are allowed which are backlogged from the good units specially purchased at the beginning of the next cycle. The separated defective units are continuously trasnsferred and sold at a reduced price from the secondary shop. This inventory model is formulated and illustrated numerically for three scenarios depending upon the time periods of the shops.     

23 excitation of Li+ by electron impact

The 1¹S→2³S excitation of Li⁺ has been studied in the Coulomb-Born-Oppenheimer approximation. The differential and the total cross sections are given at double the threshold energy.     

Interlayer force constants in high symmetry layer crystals

Symmetry adapted linear chain model has been used for the evaluation of compressional and shear interlayer force constants in MnCl₂ and GaSe from the observed zone centre and zone edge frequencies. The method is applicable to two major groups of high symmetry layer crystals having XAX and XAAX type layers.