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Kuramitsu Y Harada T Takashima M Yokoyama Y Hidaka I Iizuka N Toda T Fujimoto M Zhang X Sakaida I Okita K Oka M Nakamura K 《Electrophoresis》2006,27(8):1651-1658
Hepatocellular carcinoma (HCC) is one of the most common fatal cancers, and chronic infection with hepatitis C virus (HCV) is thought to be one of the main causes in Japan. To identify diagnostic or therapeutic biomarkers for HCC associated with HCV (HCV-HCC), we tried to elucidate the factors related to the products from cancerous tissues of HCV-infected patients. From proteomic differential display analysis of liver tissue samples from HCV-HCC cancerous tissues and corresponding non-cancerous tissues from patients, three protein spots of the same molecular mass (42 kDa), whose expression increased in well-differentiated cancerous tissues, were detected. Although their pI were different, they were identified as glutamine synthetase (GS) by PMF with MALDI-TOF MS and by Western blotting using anti-GS specific mAb. Immunohistochemical analysis showed that tumor tissue consists of two parts, GS-positive cell and GS-negative cell regions, suggesting that GS-producing cells grew in the tumor tissue as a nodule in nodules. The tryptic peptides of the most acidic GS isoform lost the signal of 899.5 Da, corresponding a peptide of SASIRIPR, and gained a signal of 1059.5 Da, which was submitted to PSD analysis. PSD analysis showed the neutral loss by elimination of two phosphate groups, supposed to be on serine residues of the 899.5-Da peptide, from serine 320 to arginine 327 in GS. PMF followed by PSD analysis is thought to be useful for the determination of phosphorylation sites of proteins showing molecular heterogeneity. 相似文献
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Dr. Kazuhisa Fujimoto Dr. Masaoki Kajino Ikumi Sakaguchi Prof. Dr. Masahiko Inouye 《Chemistry (Weinheim an der Bergstrasse, Germany)》2012,18(32):9834-9840
Diarylethene‐bridged peptides were developed to photoregulate biomolecular interactions. The peptides are made up of diarylethene‐bridged and DNA‐binding regions at their N‐ and C termini, respectively. The two regions could be independently designed and combined as desired. The α‐helicities of the peptides were photoregulated in on/off or off/on manners, and the manner depended on the positions of two ornithine (Orn) residues for cross‐linking reaction at the diarylethene‐bridged region. In the case of the on/off manner, when the diarylethene structure adopted the open form on the peptides, the peptides folded into stable α‐helices. Upon UV irradiation, the diarylethene moiety isomerized to its closed form to destabilize the helical structures. Quartz crystal microbalance (QCM) analysis showed that the open isomer strongly associated with a target DNA, as compared with the closed one. When the closed‐form peptide existing in the DNA complex was irradiated with a fluorescent lamp in the middle of the QCM monitoring, the frequency change (ΔF) was enhanced by the diarylethene photoisomerization. 相似文献
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Eri?YoshidaEmail author Motonari?Tanaka Toshikazu?Takata 《Colloid and polymer science》2005,284(1):51-57
This is the first light scattering study demonstrating that the size of micelles, the aggregation number, and the mobility of the core blocks of the micelles could be controlled by the length of the cross-linker in the micellar cores. The core cross-linked micelles were prepared using a poly[(4-pyridinemethoxy-methyl)styrene]-block-polystyrene (PPySt-b-PSt) diblock copolymer and perfluoroalkyl dicarboxylic acid. The PPySt-b-PSt copolymer formed the micelles in THF, a nonselective solvent, in the presence of the perfluoroalkyl dicarboxylic acid. The light scattering studies demonstrated that the micellar size and aggregation number were dependent on the chain length of the perfluoroalkyl dicarboxylic acid. Perfluoroazelaic acid produced micelles with a larger hydrodynamic radius and higher aggregation number than tetrafluorosuccinic acid. The micellization proceeded through the formation of the pyridinium carboxylate and the cross-linkage between the PPySt blocks via the dicarboxylic acid. The core cross-linked micelles were thermally stable and maintained its structure with changes in the temperature. A 1H NMR analysis revealed that the micelles prepared by perfluoroazelaic acid had more mobility of the core blocks than those by tetrafluorosuccinic acid. 相似文献
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n-Octyl fluoride underwent a cross-coupling reaction with n-propylmagnesium bromide in the presence of 1,3-butadiene using NiCl2 as a catalyst at room temperature to give undecane in moderate yields. This alkyl-alkyl cross-coupling proceeded more efficiently when CuCl2 was employed instead of NiCl2. Addition of 1,3-butadiene dramatically improved the yields of the coupling products from primary alkyl Grignard reagents in both Ni- and Cu-catalyzed reactions. Alkyl fluorides efficiently reacted with tertiary alkyl and phenyl Grignard reagents using CuCl2 in the absence of 1,3-butadiene to afford the coupling products in high yields. The competitive reaction of a mixture of alkyl halides (R-X; X = F, Cl, Br) with nC5H11MgBr showed that the reactivities of the halides increase in the order R-Cl < R-F < R-Br. In contrast, in the Cu-catalyzed reaction with PhMgBr, the reactivities increase in the order R-Cl < R-Br < R-F. 相似文献
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Adachi M Murata Y Sago K Nakagawa K 《Langmuir : the ACS journal of surfaces and colloids》2004,20(14):5965-5968
The formation processes of the integrated ordered mesostructure of silica at the liquid-liquid interface in laurylamine/tetraethoxysilane system were elucidated by measuring the small-angle X-ray scattering pattern every second during the formation processes using strong synchrotron radiation X-rays. Cylinders of silica were formed in random configurations in the very early stage of reactions at the liquid-liquid interface. Since these cylinders were restricted in a two-dimensional liquid-liquid interface, they aligned easily with each other and made an ordered structure composed of aligned cylinders at the interface. These ordered structures then accumulated with each other, making an integrated ordered structure, such as a hexagonal array. 相似文献
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Aoi Takeda Hiroki Akasaka Shigeo Ohshio Ikumi Toda Masayuki Nakano Hidetoshi Saitoh 《Journal of Physics and Chemistry of Solids》2012,73(11):1331-1334
To understand why amorphous carbon (a-C:H) film shows antithrombogenicity, an adsorption ability of plasma proteins on a-C:H surface was investigated. Protein adsorption is the initial process of clot formation. The protein adsorption ability on a-C:H film surface was compared by the detection using the surface plasmon resonance (SPR) phenomenon to estimate the protein adsorption. The protein adsorption abilities of a fibrinogen (Fib) and a human γ-globulin (HGG) were estimated by the SPR method using a multilayer structure of a-C:H/Au/Cr/glass. Although the adsorption of HGG for a-C:H was saturated at 32 μM in HGG concentration, the adsorption of Fib was not saturated under the detection limit of this method. These results indicated that the adsorption ability to the a-C:H film surface of Fib was higher than HGG. 相似文献
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