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排序方式: 共有273条查询结果,搜索用时 46 毫秒
221.
Miguel O. Surez-Barrera Lydia Visser Paola Rondn-Villarreal Diego F. Herrera-Pineda Juan S. Alarcn-Aldana Anke Van den Berg Jahir Orozco Efraín H. Pinzn-Reyes Ernesto Moreno Nohora J. Rueda-Forero 《Molecules (Basel, Switzerland)》2021,26(24)
Bacillus thuringiensis (Bt) is a bacterium capable of producing Cry toxins, which are recognized for their bio-controlling actions against insects. However, a few Bt strains encode proteins lacking insecticidal activity but showing cytotoxic activity against different cancer cell lines and low or no cytotoxicity toward normal human cells. A subset of Cry anticancer proteins, termed parasporins (PSs), has recently arisen as a potential alternative for cancer treatment. However, the molecular receptors that allow the binding of PSs to cells and their cytotoxic mechanisms of action have not been well established. Nonetheless, their selective cytotoxic activity against different types of cancer cell lines places PSs as a promising alternative treatment modality. In this review, we provide an overview of the classification, structures, mechanisms of action, and insights obtained from genetic modification approaches for PS proteins. 相似文献
222.
Francesco Colizzi Adam Hospital Sanja Zivanovic Modesto Orozco 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(12):3799-3803
The energetics of intramolecular recognition processes are governed by the balance of pre‐organization and flexibility, which is often difficult to measure and hard to predict. Using classical MD simulations, we predict and quantify the effective strength of intramolecular hydrogen bonds between donor and acceptor sites separated by a variable alkyl linker in several solvents and crowded solutions. The balance of entropic and enthalpic contributions poses a solvent‐dependent limit to the occurrence of intramolecular H‐bonding. Still, free energies show a constant offset among different solvents with, for example, a 13 kJ mol?1 difference between water and chloroform. Molecular crowding shows little effect on the thermodynamic equilibrium, but induces variations on the H‐bond kinetics. The results are in quantitative agreement with experiments in chloroform and showcase a general strategy to investigate molecular interactions in different environments, extending the limits of current experiments towards the prospective prediction of H‐bond interactions in a variety of contexts. 相似文献
223.
Friederike M. Dannheim Stephen J. Walsh Carolina T. Orozco Anders Hjgaard Hansen Jonathan D. Bargh Sophie E. Jackson Nicholas J. Bond Jeremy S. Parker Jason S. Carroll David R. Spring 《Chemical science》2022,13(30):8781
Antibody–drug conjugates (ADCs) are valuable therapeutic entities which leverage the specificity of antibodies to selectively deliver cytotoxins to antigen-expressing targets such as cancer cells. However, current methods for their construction still suffer from a number of shortcomings. For instance, using a single modification technology to modulate the drug-to-antibody ratio (DAR) in integer increments while maintaining homogeneity and stability remains exceptionally challenging. Herein, we report a novel method for the generation of antibody conjugates with modular cargo loading from native antibodies. Our approach relies on a new class of disulfide rebridging linkers, which can react with eight cysteine residues, thereby effecting all-in-one bridging of all four interchain disulfides in an IgG1 antibody with a single linker molecule. Modification of the antibody with the linker in a 1 : 1 ratio enabled the modulation of cargo loading in a quick and selective manner through derivatization of the linker with varying numbers of payload attachment handles to allow for attachment of either 1, 2, 3 or 4 payloads (fluorescent dyes or cytotoxins). Assessment of the biological activity of these conjugates demonstrated their exceptional stability in human plasma and utility for cell-selective cytotoxin delivery or imaging/diagnostic applications.Tetra-divinylpyrimidine (TetraDVP) linkers offer a method for the generation of antibody conjugates with modular cargo loading and excellent stability via all-in-one disulfide bridging. 相似文献
224.
W. Strecker und F. Orozco Diaz 《Fresenius' Journal of Analytical Chemistry》1925,67(9):321-341
Ohne Zusammenfassung 相似文献
225.
Francisco C.S. Chernicharo Lucas Modesto‐Costa Itamar Borges 《Journal of mass spectrometry : JMS》2020,55(6)
Tabun (ethyl N,N‐dimethylphosphoramidocyanidate), or GA, is a chemical warfare nerve agent produced during the World War II. The synthesis of its analogs is rather simple; thus, it is a significant threat. Furthermore, experiments with tabun and other nerve agents are greatly limited by the involved life risks and the severe restrictions imposed by the Chemical Weapons Convention. For these reasons, accurate theoretical assignment of fragmentation pathways can be especially important. In this work, we employ the Quantum Chemistry Electron Ionization Mass Spectra method, which combines molecular dynamics, quantum chemistry methods, and stochastic approaches, to accurately investigate the electron ionization/mass spectrometry (EI/MS) fragmentation spectrum and pathways of the tabun molecule. We found that different rearrangement reactions occur including a McLafferty involving the nitrile group. An essential and characteristic pathway for identification of tabun and analogs, a two‐step fragmentation producing the m/z 70 ion, was confirmed. The present results will be also useful to predict EI/MS spectrum and fragmentation pathways of other members of the tabun family, namely, the O‐alkyl/cycloalkyl N,N‐dialkyl (methyl, ethyl, isopropyl, or propyl) phosphoramidocyanidates. 相似文献
226.
JETP Letters - A necessary condition for reflection positivity is that the free propagator be positive. Contrary to recent claims in the literature, we show that this is indeed the case for a large... 相似文献
227.
The new first order, rheonomic, κ–supersymmetric formalism recently introduced by us for the world‐volume action of the D3 brane is extended to the case of D5 branes. This extension requires the dual formulation of the Free Differential Algebra of type IIB supergravity in terms of 6–form gauge potentials which was so far missing and is given here. Furthermore relying on our new approach we are able to write the D5 world volume action in a manifestly SL(2,ℝ) covariant form. This is important in order to solve the outstanding problem of finding the appropriate boundary actions of D3–branes on smooth ALE manifolds with twisted fields. The application of our results to this problem is however postponed to a subsequent publication. 相似文献
228.
Marie-Florence Grenier-Loustalot Laurence Orozco Philippe Grenier 《Macromolecular Symposia》1987,9(1):193-202
The course of model reactions of resins containing N-(epoxy)2 groups with an aromatic amine was followed by measurements and high resolution 13C NMR spectroscopy in the liquid state. A reaction mechanism and a kinetic model are proposed according to the structure of the resins. 相似文献
229.
E. A. Juarez‐Arellano I. Rosales G. V. Gamboa‐Espinosa J. A. Lara L. Bucio E. Orozco 《Crystal Research and Technology》2004,39(10):833-839
Micro‐crystals of two new compounds EuMnGe2O7 and SmMnGe2O7 were grown performing the flux method in a double‐spherical mirror furnace. One valuable advantage of this system was that the heating profile could be modified easily adjusting lamp positions and orientation as well. The micrometric crystals were observed and analyzed for chemical composition by scanning electron microscopy and energy dispersive spectroscopy. This furnace is perfectly suitable to grow at low price, low temperature and short time new materials as a single crystal for basic research or to obtain raw material. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
230.
Alisa A. Nevskaya Lada V. Anikina Rosa Purgatorio Marco Catto Orazio Nicolotti Modesto de Candia Leonardo Pisani Tatiana N. Borisova Almira R. Miftyakhova Aleksey V. Varlamov Elena Yu. Nevskaya Roman S. Borisov Leonid G. Voskressensky Cosimo D. Altomare 《Molecules (Basel, Switzerland)》2021,26(2)
Marine alkaloids belonging to the lamellarins family, which incorporate a 5,6-dihydro-1-phenylpyrrolo[2,1-a]isoquinoline (DHPPIQ) moiety, possess various biological activities, spanning from antiviral and antibiotic activities to cytotoxicity against tumor cells and the reversal of multidrug resistance. Expanding a series of previously reported imino adducts of DHPPIQ 2-carbaldehyde, novel aliphatic and aromatic Schiff bases were synthesized and evaluated herein for their cytotoxicity in five diverse tumor cell lines. Most of the newly synthesized compounds were found noncytotoxic in the low micromolar range (<30 μM). Based on a Multi-fingerprint Similarity Search aLgorithm (MuSSeL), mainly conceived for making protein drug target prediction, some DHPPIQ derivatives, especially bis-DHPPIQ Schiff bases linked by a phenylene bridge, were prioritized as potential hits addressing Alzheimer’s disease-related target proteins, such as cholinesterases (ChEs) and monoamine oxidases (MAOs). In agreement with MuSSeL predictions, homobivalent para-phenylene DHPPIQ Schiff base 14 exhibited a noncompetitive/mixed inhibition of human acetylcholinesterase (AChE) with Ki in the low micromolar range (4.69 μM). Interestingly, besides a certain inhibition of MAO A (50% inhibition of the cell population growth (IC50) = 12 μM), the bis-DHPPIQ 14 showed a good inhibitory activity on self-induced β-amyloid (Aβ)1–40 aggregation (IC50 = 13 μM), which resulted 3.5-fold stronger than the respective mono-DHPPIQ Schiff base 9. 相似文献