A dialectical framework for problem structuring and information technology

This paper proposes that problem structuring is characterized by dialectical processes, and that different problem situations call for differing emphasis on the organizing versus disorganizing potential of information technologies. The term ‘dialectical’ emphasizes the conflicting forces of structuring and unstructuring in processes of problem solving. A case study of an enterprise software conversion project examines four processes: structuring, unstructuring, groping, and adjusting. Applying the dialectic perspective to the classic Gorry and Scott-Morton framework yields propositions as to which of these four processes should be emphasized by information systems addressing different types of problems. Implications for the design of decision support systems and for future research are discussed.     

Identification of a set of genes showing regionally enriched expression in the mouse brain

Background

We have recorded responses from single neurons in murine visual cortex to determine the effectiveness of the input from the two murine cone photoreceptor mechanisms and whether there is any unique selectivity for cone inputs at this higher region of the visual system that would support the possibility of colour vision in mice. Each eye was stimulated by diffuse light, either 370 (strong stimulus for the ultra-violet (UV) cone opsin) or 505 nm (exclusively stimulating the middle wavelength sensitive (M) cone opsin), obtained from light emitting diodes (LEDs) in the presence of a strong adapting light that suppressed the responses of rods.

Results

Single cells responded to these diffuse stimuli in all areas of striate cortex. Two types of responsive cells were encountered. One type (135/323 – 42%) had little to no spontaneous activity and responded at either the on and/or the off phase of the light stimulus with a few impulses often of relatively large amplitude. A second type (166/323 – 51%) had spontaneous activity and responded tonically to light stimuli with impulses often of small amplitude. Most of the cells responded similarly to both spectral stimuli. A few (18/323 – 6%) responded strongly or exclusively to one or the other spectral stimulus and rarely in a spectrally opponent manner.

Conclusion

Most cells in murine striate cortex receive excitatory inputs from both UV- and M-cones. A small fraction shows either strong selectivity for one or the other cone mechanism and occasionally cone opponent responses. Cells that could underlie chromatic contrast detection are present but extremely rare in murine striate cortex.     

Analytical methods for human biomonitoring of pesticides. A review

Biomonitoring of both currently-used and banned-persistent pesticides is a very useful tool for assessing human exposure to these chemicals. In this review, we present current approaches and recent advances in the analytical methods for determining the biomarkers of exposure to pesticides in the most commonly used specimens, such as blood, urine, and breast milk, and in emerging non-invasive matrices such as hair and meconium. We critically discuss the main applications for sample treatment, and the instrumental techniques currently used to determine the most relevant pesticide biomarkers. We finally look at the future trends in this field.     

General Properties of the Nernst-Planck-Poisson-Boltzmann System Describing Electrocapillary Effects in Porous Media

The Nernst-Planck-Poisson-Boltzmann system describes the evolution of ionic concentrations and electrocapillarity effects in porous media. The aim of this paper is a theoretical study of various drift-diffusion modellings. The well-posedness of the systems is proved and some qualitative properties of the global solution are shown to be satisfied (energy law, entropy law in the weak sense of Lyapunov functions, stationary states, Maxwellian distribution, influence of an external electric field). Moreover, some explicit solutions are established in the one-dimensional case.     

Analysis of trace levels of pesticides in rainwater by SPME and GC-tandem mass spectrometry after derivatisation with PFFBr

Solid-phase microextraction (SPME) was used for the analysis of some pesticides (bromoxynil, chlorotoluron, diuron, isoproturon, 2,4-MCPA, MCPP and 2,4-D) in rainwater after derivatisation with PFBBr and gas chromatography-ion trap mass spectrometry. The derivatisation procedure was optimized by testing different methods: direct derivatisation in the aqueous phase followed by SPME extraction, on-fibre derivatisation and derivatisation in the injector. The best result was obtained by headspace coating the PDMS/DVB fibre with PFBBr for 10 min followed by direct SPME extraction for 60 min at 68 °C (pH 2 and 75% NaCl). Good detection limits were obtained for all the compounds: these ranged between 10 and 1,000 ng L⁻¹ with a relatively high uncertainty due to the combination of derivatisation and SPME extraction steps. The optimized procedure was applied to the analysis of pesticides in rainwater and results obtained shows that this method is a fast and simple technique to assess the spatial and temporal variations of concentrations of pesticides in rainwater.     

Separation of molecular tracers sorbed onto atmospheric particulate matter by flash chromatography

In to order increase sensitivity and to reduce the background induced by matrix effects, a method was developed that uses flash chromatography to separate various compounds present in atmospheric aerosol samples prior to their analysis with different analytical techniques (GC–MS, GC–FID, HPLC). For this purpose, flash chromatography using a 4 g silica gel column crossed by eluent at a flow rate of 20 mL min⁻¹ was used. An eluent with enhanced polarity is needed to separate nonpolar (linear and branched alkanes), semipolar (PAH, nitro-PAH and cholesterol) and polar (methoxyphenols, alkanoic acids, and levoglucosan) compounds. Three combinations of solvents were used: hexane for the nonpolar fraction (F1), toluene/hexane for the semipolar fraction (F2) and dimethylformamide for the polar fraction (F3). The use of different eluents for each fraction allows separation of the sample to be accomplished with good repeatability and satisfying yields [85 ± 5% for F1, 81 ± 8% (PAHs), 89 ± 6% (nitro-PAHs) and 74 ± 7% (cholesterol) for F2 and 79 ± 7% (n-alkanoic acids), 40 ± 11% (methoxyphenols) and 77 ± 6% (levoglucosan) for F3]. The methoxyphenol yields were low due to losses during the concentration/evaporation step. This method was then applied to analyse the organic composition of particles collected at an urban site in Strasbourg (France).     

Neuroinflammation Modulation via α7 Nicotinic Acetylcholine Receptor and Its Chaperone,RIC-3

Nicotinic acetylcholine receptors (nAChRs) are widely expressed in or on various cell types and have diverse functions. In immune cells nAChRs regulate proliferation, differentiation and cytokine release. Specifically, activation of the α7 nAChR reduces inflammation as part of the cholinergic anti-inflammatory pathway. Here we review numerous effects of α7 nAChR activation on immune cell function and differentiation. Further, we also describe evidence implicating this receptor and its chaperone RIC-3 in diseases of the central nervous system and in neuroinflammation, focusing on multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Deregulated neuroinflammation due to dysfunction of α7 nAChR provides one explanation for involvement of this receptor and of RIC-3 in neurodegenerative diseases. In this review, we also provide evidence implicating α7 nAChRs and RIC-3 in neurodegenerative diseases such as Alzheimer’s disease (AD) and Parkinson’s disease (PD) involving neuroinflammation. Besides, we will describe the therapeutic implications of activating the cholinergic anti-inflammatory pathway for diseases involving neuroinflammation.     

On the definition of the stress tensor in granular media

This paper investigates the definition of the stress tensor within a granular assembly, when inertial effects are likely to occur. It is shown that the stress tensor can be expressed as a sum of two terms. A first term corresponds to the standard definition of the stress, according to the Love–Weber formula; this term is related to the contact forces existing within adjoining particles. A second term accounts for dynamic effects related to rotation velocities and accelerations of the particles. These results are checked from discrete numerical simulations in order to examine in which context the contribution of inertial effects should not be omitted. With this aim, the simulation of a granular specimen collapse and then a silo discharge is considered.     

Analytic Calculation of Capillary Bridge Properties Deduced as an Inverse Problem from Experimental Data

In this work, we propose an original resolution of Young–Laplace equation for capillary doublets from an inverse problem. We establish a simple explicit criterion based on the observation of the contact point, the wetting angle and the gorge radius, to classify in an exhaustive way the nature of the surface of revolution. The true shape of the admissible static bridges surface is described by parametric equations; this way of expressing the profile is practical and well efficient for calculating the binding forces, areas and volumes. Moreover, we prove that the inter-particle force may be evaluated on any section of the capillary bridge and constitutes a specific invariant.     

Homogenization of the Nernst-Planck-Poisson System by Two-Scale Convergence

This work focuses on performing the homogenization of the Nernst-Planck-Poisson system, in order to obtain an efficient modelling of the ionic transfer and electrocapillary effects at the macroscopic scale for saturated porous media. The homogenization is performed with the powerful two-scale convergence method, which enables to obtain both the homogenized model and the associated convergence result.