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31.
32.
Mike Friedrich Revaz Nozadze Sandra de Keijzer Ralf Steinmeyer Vladimir Ermolayev Gregory S. Harms 《Journal of fluorescence》2018,28(1):29-39
Single molecule detection and tracking provides at times the only possible method to observe the interactions of low numbers of biomolecules, inlcuding DNA, receptors and signal mediating proteins in living systems. However, most existing imaging methods do not enable both high sensitivity and non-invasive imaging of large specimens. In this study we report a new setup for selective plane illumination microscopy (SPIM), which enables fast imaging and single molecule tracking with the resolution of confocal microscopy and the optical penetration beyond 300 μm. We detect and report our instrumental figures of merit, control values of fluorescence properties of single nano crystals in comparison to both standard widefield configurations, and also values of nanocrystals in multicellular “fruiting bodies” of Dictyostelium, an excellent control as a model developmental system. In the Dictyostelium , we also report some of our first tracking of single nanocrystals with SPIM. The new SPIM setup represents a new technique, which enables fast single molecule imaging and tracking in living systems. 相似文献
33.
Zeidler A Salmon PS Fischer HE Neuefeind JC Simonson JM Lemmel H Rauch H Markland TE 《Physical review letters》2011,107(14):145501
The method of oxygen isotope substitution in neutron diffraction is introduced as a site specific structural probe. It is employed to measure the structure of light versus heavy water, thus circumventing the assumption of isomorphism between H and D as used in more traditional neutron diffraction methods. The intramolecular and intermolecular O-H and O-D pair correlations are in excellent agreement with path integral molecular dynamics simulations, both techniques showing a difference of ?0.5% between the O-H and O-D intramolecular bond distances. The results support the validity of a competing quantum effects model for water in which its structural and dynamical properties are governed by an offset between intramolecular and intermolecular quantum contributions. 相似文献
34.
Abe F Albrow MG Amidei D Antos J Anway-Wiese C Apollinari G Areti H Atac M Auchincloss P Azfar F Azzi P Bacchetta N Badgett W Bailey MW Bao J de Barbaro P Barbaro-Galtieri A Barnes VE Barnett BA Bartalini P Bauer G Baumann T Bedeschi F Behrends S Belforte S Bellettini G Bellinger J Benjamin D Benlloch J Bensinger J Benton D Beretvas A Berge JP Bertolucci S Bhatti A Biery K Binkley M Bird F Bisello D Blair RE Blocker C Bodek A Bokhari W Bolognesi V Bortoletto D Boswell C Boulos T Brandenburg G 《Physical review letters》1994,73(20):2662-2666
35.
Abe F Albrow MG Amendolia SR Amidei D Antos J Anway-Wiese C Apollinari G Areti H Atac M Auchincloss P Azfar F Azzi P Bacchetta N Badgett W Bailey MW Bao J de Barbaro P Barbaro-Galtieri A Barnes VE Barnett BA Bartalini P Bauer G Baumann T Bedeschi F Behrends S Belforte S Bellettini G Bellinger J Benjamin D Benlloch J Bensinger J Benton D Beretvas A Berge JP Bertolucci S Bhatti A Biery K Binkley M Bird F Bisello D Blair RE Blocker C Bodek A Bokhari W Bolognesi V Bortoletto D Boswell C Boulos T 《Physical review letters》1995,75(6):1012-1016
36.
Building activities are recognised to produce coarse particulate matter but less is known about the release of airborne ultrafine
particles (UFPs; those below 100 nm in diameter). For the first time, this study has investigated the release of particles
in the 5–560 nm range from three simulated building activities: the crushing of concrete cubes, the demolition of old concrete
slabs, and the recycling of concrete debris. A fast response differential mobility spectrometer (Cambustion DMS50) was used
to measure particle number concentrations (PNC) and size distributions (PNDs) at a sampling frequency of 10 Hz in a confined
laboratory room providing controlled environment and near–steady background PNCs. The sampling point was intentionally kept
close to the test samples so that the release of new UFPs during these simulated processes can be quantified. Tri–modal particle
size distributions were recorded for all cases, demonstrating different peak diameters in fresh nuclei (<10 nm), nucleation
(10–30 nm) and accumulation (30–300 nm) modes for individual activities. The measured background size distributions showed
modal peaks at about 13 and 49 nm with average background PNCs ~1.47 × 104 cm−3. These background modal peaks shifted towards the larger sizes during the work periods (i.e. actual experiments) and the
total PNCs increased between 2 and 17 times over the background PNCs for different activities. After adjusting for background
concentrations, the net release of PNCs during cube crushing, slab demolition, and ‘dry’ and ‘wet’ recycling events were measured
as ~0.77, 19.1, 22.7 and 1.76 (×104) cm−3, respectively. The PNDs were converted into particle mass concentrations (PMCs). While majority of new PNC release was below
100 nm (i.e. UFPs), the bulk of new PMC emissions were constituted by the particles over 100 nm; ~95, 79, 73 and 90% of total
PNCs, and ~71, 92, 93 and 91% of total PMCs, for cube crushing, slab demolition, dry recycling and wet recycling, respectively.
The results of this study firmly elucidate the release of UFPs and raise a need for further detailed studies and designing
health and safety related exposure guidelines for laboratory workplaces and operational building sites. 相似文献
37.
A modified spin-flip model is used to investigate the stability of optically-pumped quantum-dot spin-vertical cavity surface-emitting lasers. in terms of pump intensity and polarization. The stability maps exhibit pronounced polarization switching in the stable regions. Periodic oscillations in unstable regions are attributed to competition between spin relaxation and birefringence. 相似文献
38.
When a liquid drop contacts a wettable surface, the liquid spreads over the solid to minimize the total surface energy. The first moments of spreading tend to be rapid. For example, a millimeter-sized water droplet will wet an area having the same diameter as the drop within a millisecond. For perfectly wetting systems, this spreading is inertially dominated. Here we identify that even in the presence of a contact line, the initial wetting is dominated by inertia rather than viscosity. We find that the spreading radius follows a power-law scaling in time where the exponent depends on the equilibrium contact angle. We propose a model, consistent with the experimental results, in which the surface spreading is regulated by the generation of capillary waves. 相似文献
39.
40.
The influence of an asymmetric periodic grooved cell surface on the 2D static director configuration of a nematic liquid crystal has been investigated. The minimum in the Frank-Oseen free energy was solved numerically with the Rapini-Papoular form of the surface anchoring energy at the nematic-grating interface. Results are presented for the variation of pretilt angle in the tilted bulk director field as a function of the surface groove depth, pitch and asymmetry and the bulk parameters. The simulations demonstrate the existence of two energetically degenerate high and low pretilted bulk alignment configurations. The pretilt values in these two regimes and also for the low tilt regime with finite surface anchoring are consistent with experimental results. An effective increase in the resolution of the model is obtained by using an irregular grid to describe the surface profile. 相似文献