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It can be difficult for the voice clinician to observe or measure how a patient uses his voice in a noisy environment. We consider here a novel method for obtaining this information in the laboratory. Worksite noise and filtered white noise were reproduced over high-fidelity loudspeakers. In this noise, 11 subjects read an instructional text of 1.5 to 2 minutes duration, as if addressing a group of people. Using channel estimation techniques, the site noise was suppressed from the recording, and the voice signal alone was recovered. The attainable noise rejection is limited only by the precision of the experimental setup, which includes the need for the subject to remain still so as not to perturb the estimated acoustic channel. This feasibility study, with 7 female and 4 male subjects, showed that small displacements of the speaker's body, even breathing, impose a practical limit on the attainable noise rejection. The noise rejection was typically 30 dB and maximally 40 dB down over the entire voice spectrum. Recordings thus processed were clean enough to permit voice analysis with the long-time average spectrum and the computerized phonetogram. The effects of site noise on voice sound pressure level, fundamental frequency, long-term average spectrum centroid, phonetogram area, and phonation time were much as expected, but with some interesting differences between females and males. 相似文献
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645.
A new multi-residue method for analysis of pesticide residues in fruit and vegetables using liquid chromatography with tandem mass spectrometric detection 总被引:12,自引:0,他引:12
A new multi-residue method for determination of pesticide residues in a wide variety of fruit and vegetables, using the National Food Administration (NFA) ethyl acetate extraction and determination by means of LC-MS/MS, is presented. The method includes pesticides normally detected by LC-UV or LC-fluorescence such as benzimidazoles, carbamates, N-methylcarbamates and organophosphorus compounds with an oxidisable sulphide group as well. After extraction with ethyl acetate, the extract is concentrated and an aliquot of the extract is evaporated to dryness and redissolved in methanol before injection on LC-MS/MS. The method has been validated for 57 different pesticides and metabolites. Representative species from different commodity groups were chosen as matrices in order to study the influence from different matrices on recoveries. The fortification levels studied were 0.01-0.5 mg kg(-1). Matrix effects were tested for all matrices by means of standard addition to blank extracts. The matrix effect, expressed as signal in solvent compared to signal in matrix, was in general found to be small. The obtained recoveries are, with a few exceptions, in the range 70-100%. The proposed method is quick and straightforward and no additional clean-up steps are needed. The method can be used for the analysis of all 57 pesticides in one single determination step at 0.01 mg kg(-1). 相似文献
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647.
Summary The organic solvent N-methylformamide (NMF) has been used as a separation medium in capillary electrophoresis. The advantageous properties of this compound are its high dielectric constant, high solubilizing power and low conductivity, as well as its amphiprotic character. It was shown that, unlike for most organic solvents, the electroosmotic flow is substantial. It was found to be possible to utilize NMF without added electrolyte. Field strengths exceeding 1000 volts/cm could be employed, while a low current was maintained and it was thus possible to obtain rapid analyses. Also, the properties of NMF allowed the analysis of substances with a low solubility in aqueous media. These features are exemplified by separation of carboxylic acids and pharmaceuticals. Excellent reproducibility of migration and no sign of electrical breakdown were observed, even under high field strength conditions.Presented at the Sixth International Symposium on High Performance Capillary Electrophoresis, Jan. 31–Feb. 9, 1994, San Diego, USA, Poster #P-113. 相似文献
648.
Wallner FK Norberg HA Johansson AI Mogemark M Elofsson M 《Organic & biomolecular chemistry》2005,3(2):309-315
Synthetic glycolipids with defined structures are important tools in the study of glycolipid biology. In this paper we describe a solid-phase synthesis of three galactosylated serine-based glycosphingolipid analogues using the novel linker 2-fluoro-4-(hydroxymethyl)-phenoxyacetic acid. Gel-phase (19)F-NMR spectroscopy was used to measure the yield and stereochemical outcome of the solid-phase glycosylations. Under NIS-TfOH promotion, alpha- and beta-selective glycosylations were performed at room temperature with thioglycoside donors carrying fluorine labelled protective groups. Finally, the glycolipids were covalently linked to microtiter plates and labelled lectins with different selectivity for alpha- and beta-galactosides could bind to the glycolipid arrays. 相似文献
649.
The 26,23-lactone derivative of 1alpha,25-dihydroxyvitamin D3, TEI-9647, is a partial antagonist of the of human vitamin D receptor (VDR). However, we found that TEI-9647 in rat cells behaves as a weak VDR agonist. This behavior could be mimicked in human cells by the double mutagenesis of human VDR (specifically C403S and C410N). The increased agonistic action of TEI-9647 correlates to a gain in the interaction of the VDR with coactivator protein and a decreased stabilization of the antagonistic conformation of the receptor. Molecular dynamics simulations indicated that TEI-9647 acts as antagonist of human VDR by reducing the stability of helix 12 of the ligand binding domain. In contrast, N410 of the rat VDR stabilized, via backbone contacts, the interaction between helices 11 and 12. This results in TEI-9647 becoming a weak agonist in this organism. 相似文献
650.
Four orders of magnitude rate increase in artificial enzyme-catalyzed aryl glycoside hydrolysis 总被引:1,自引:0,他引:1
Ortega-Caballero F Bjerre J Laustsen LS Bols M 《The Journal of organic chemistry》2005,70(18):7217-7226
[reaction: see text] (6AR,6DR)-6A,6D-Di-C-cyano-beta-cyclodextrin (1) and 6A,6D-di-C-cyano-alpha-cyclodextrin (2) were synthesized and shown to catalyze hydrolysis of aryl glycosides into glucose and phenol with a reaction following Michaelis-Menten kinetics. At pH 8.0 and 59 degrees C hydrolysis of 4-nitrophenyl alpha-glucopyranoside was catalyzed by 1 with KM = 10.5 +/- 1.5 mM, kcat = 1.42(+/-0.09) x 10(-4) s(-1), and kcat/kuncat = 7922. Catalysis was observed with a concentration of 1 as low as 10 microM. Hydrolysis of the other aryl glycosides containing stereochemical variation in the sugar-moiety and 4-nitro-, 2-nitro-, 2-aldehydo-, and 2,4-dinitro- were also catalyzed by 1 and 2 with kcat/kuncat ranging from 4 to 7100. Hydrolysis of a phenyl beta-d-glucoside or the thioglycoside tolylthio beta-D-glucoside was also catalyzed. From a series of prepared analogues of 1 it was found that the catalysis was associated with the hydroxyl groups alpha to the nitril groups. The monocyanohydrin 6-C-cyano-beta-cyclodextrin (3) was also found to catalyze the hydrolysis of 4-nitrophenyl beta-glucopyranoside with kcat/kuncat = 1356. It was proposed that the cyclodextrin cyanohydrins 1-3 catalyze the hydrolysis by general acid catalysis on the bound substrate. 相似文献