We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths. 相似文献
Accurate in silico models for predicting aqueous solubility are needed in drug design and discovery and many other areas of chemical research. We present a statistical modeling of aqueous solubility based on measured data, using a Gaussian Process nonlinear regression model (GPsol). We compare our results with those of 14 scientific studies and 6 commercial tools. This shows that the developed model achieves much higher accuracy than available commercial tools for the prediction of solubility of electrolytes. On top of the high accuracy, the proposed machine learning model also provides error bars for each individual prediction. 相似文献
The size and shape of novel partially fluorinated gemini surfactant 1,2-bis[dimethyl-(3-perfluoroalkyl-2-hydroxypropyl)ammonium]ethane bromide (CnFC3-2-C3CnF, where n=4, 6, and 8) were investigated in aqueous solution by means of light scattering and transmission electron microscopy (TEM). The sizes of these molecular aggregates changed with increasing carbon number of the alkyl chain and concentration. For example, the apparent hydrodynamic radius by dynamic light scattering was 18 nm at a concentration of cmcx5 for n=4, 115 nm at the cmcx15 for n=6, and 62 nm at the cmcx30 for n=8, at 298.2 K. The shapes of CnFC3-2-C3CnF aggregates drastically changed with the alkyl chain length; the aggregates were mainly in the form of large or irregular small aggregates (n=4), string-like aggregates (n=6), and vesicles (n=8). The bromide-ion activity was measured using a bromide-ion-selective electrode to determine the degree of counterion binding to the aggregates. The degree of counterion binding to aggregate was very small compared with that in the typical hydrogenated gemini surfactants. These results indicated that the small curvature of large aggregates was not influenced by an electrostatic repulsion between the cationic head groups in the case of the bulky molecular volume of fluorinated gemini surfactants. 相似文献
To improve interfacial phenomena of poly(dimethylsiloxane) (PDMS) as biomaterials, well-defined triblock copolymers were prepared as coating materials by reversible addition-fragmentation chain transfer (RAFT) controlled polymerization. Hydroxy-terminated poly(vinylmethylsiloxane-co-dimethylsiloxane) (HO–PVlDmMS–OH) was synthesized by ring-opening polymerization. The copolymerization ratio of vinylmethylsiloxane to dimethylsiloxane was 1/9. The molecular weight of HO–PVlDmMS–OH ranged from (1.43 to 4.44) × 104, and their molecular weight distribution (Mw/Mn) as determined by size-exclusion chromatography equipped with multiangle laser light scattering (SEC-MALS) was 1.16. 4-Cyanopentanoic acid dithiobenzoate was reacted with HO–PVlDmMS–OH to obtain macromolecular chain transfer agents (macro-CTA). 2-Methacryloyloxyethyl phosphorylcholine (MPC) was polymerized with macro-CTAs. The gel-permeation chromatography (GPC) chart of synthesized polymers was a single peak and Mw/Mn was relatively narrow (1.3–1.6). Then the poly(MPC) (PMPC)–PVlDmMS–PMPC triblock copolymers were synthesized. The molecular weight of PMPC in a triblock copolymer was easily controllable by changing the polymerization time or the composition of the macro-CTA to a monomer in the feed. The synthesized block copolymers were slightly soluble in water and extremely soluble in ethanol and 2-propanol.
Surface modification was performed via hydrosilylation. The block copolymer was coated on the PDMS film whose surface was pretreated with poly(hydromethylsiloxane). The surface wettability and lubrication of the PDMS film were effectively improved by immobilization with the block copolymers. In addition, the number of adherent platelets from human platelet-rich plasma (PRP) was dramatically reduced by surface modification. Particularly, the triblock copolymer having a high composition ratio of MPC units to silicone units was effective in improving the surface properties of PDMS.
By selective decomposition of the Si–H bond at the surface of the PDMS substrate by irradiation with UV light, the coating region of the triblock copolymer was easily controlled, resulting in the fabrication of micropatterns. On the surface, albumin adsorption was well manipulated. 相似文献
A new thermodynamic model is proposed in order to account for the high spin low spin conversion in metal-organic polymers. The model, based on the idea that the spin conversion occurs in interacting
domains of like-spin metal ions, allows to explain most of the important features of various types of spin conversion. The
sine qua non condition of the existence of spin transitions with hysteresis is obtained. In the case of very large cooperativity, the
model predicts unusual behaviour of the spin conversion system due to a low-temperature metastable high spin state. Existence
of such a state is interesting in the context of the light induced excited spin state trapping recently observed in some ferrous
compounds. The model is applied to interpret the spin transition in polycrystalline ferrous polymer [Fe1-yCuy (Htrz)2trz] (BF4) with y = 0.00, 0.01 and 0.10, detected by differential scanning calorimetry, optical reflectivity and electron paramagnetic resonance.
The domain size and the interaction energy between the domains are estimated as, respectively, n = 11 and for the y = 0 compound. As the copper content is growing, n and tend to decrease, resulting in transformations of the shape of hysteresis loop which becomes less steep, narrows and shifts
to lower temperatures. The electron paramagnetic resonance gives further evidence of the presence of like-spin domains.
Received 27 November 1998 and Received in final form 19 April 1999 相似文献
An orientation reversing involution of a topological compact genus surface induces an antiholomorphic involution of the Teichmüller space of genus g Riemann surfaces. Two such involutions and are conjugate in the mapping class group if and only if the corresponding orientation reversing involutions and of are conjugate in the automorphism group of . This is equivalent to saying that the quotient surfaces and are homeomorphic. Hence the Teichmüller space has distinct antiholomorphic involutions, which are also called real structures of ([7]). This result is a simple fact that follows from Royden's theorem ([4]) stating that the the mapping class group is
the full group of holomorphic automorphisms of the Teichmüller space (). Let and be two real structures that are not conjugate in the mapping class group. In this paper we construct a real analytic diffeomorphism
such that
This mapping d is a product of full and half Dehn–twists around certain simple closed curves on the surface . This has applications to the moduli spaces of real algebraic curves. A compact Riemann surface admitting an antiholomorphic involution is a real algebraic curve of the topological type . All fixed–points of the real structure of the Teichmüller space , are real curves of the above topological type and every real curve of that topological type is represented by an element
of the fixed–point set of . The fixed–point set is the Teichmüller space of real algebraic curves of the corresponding topological type. Given two different real structures
and , let d the the real analytic mapping satisfying (1). It follows that d maps onto and is an explicit real analytic diffeomorphism between these Teichmüller spaces.
Received 8 December 1997; accepted 12 August 1998 相似文献
A technique for all-optical laser cleaning and surface quality monitoring of concave metal surfaces is suggested. Contaminated pharmaceutical punches from a tablet compression machine were cleaned using high-energy laser pulses. Image information obtained from a diffractive-optical-element-based sensor was used in inspection of the surface quality change of the concave punches due to laser cleaning. Alternative method for mechanical cleaning of punches is introduced, which is based on simultaneous laser cleaning and surface quality inspection of the punches. 相似文献
A continuous process for the synthesis of mesoporous silicates such as MCM-41 at room temperature has been achieved by employing a tubular reactor in which tetraethoxysilane was used as the silicate source (see schematic representation below). The formation of the hexagonal mesophase, which was monitored by using in situ XRD at different points of the tube, was completed in three minutes after mixing of the reactants. The product was stable to calcination, and N2-sorption measurements confirmed the high surface area and narrow pore-size distribution. 相似文献