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Gorzelle BM Hoffman AK Keyes MH Gray DN Ray DG Sanders CR 《Journal of the American Chemical Society》2002,124(39):11594-11595
Amphipathic polymers ("amphipols") were introduced several years ago (Tribet, C.; Audebert, R.; Popot, J.-L. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 15047-15050) as an alternative method for solubilizing integral membrane proteins in stable, nativelike conformations. However, direct maintenance of full membrane protein functionality in amphipol solutions has not previously been demonstrated in the absence of added lipid or detergent. In this contribution, the first zwitterionic amphipol "PMAL-B-100" is introduced. PMAL-B-100 not only maintains membrane protein structure and solubility, but also supports the full catalytic activity of an integral membrane enzyme, diacylglycerol kinase, in the complete absence of additional lipid or detergent. All of the roles which a lipid bilayer normally plays in maintaining diacylglycerol kinase's structure and in facilitating catalysis are satisfied by the environment and interactions supplied by PMAL-B-100. 相似文献
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Karim Boulabiar Gerard Buskes Melvin Henriksen 《Journal of Mathematical Analysis and Applications》2003,280(2):334-349
In J. Math. Anal. Appl. 12 (1995) 258–265, Araujo et al. proved that for any linear biseparating map from C(X) onto C(Y), where X and Y are completely regular, there exist ω in C(Y) and an homeomorphism h from the realcompactification vX of X onto vY, such that The compact version of this result was proved before by Jarosz in Bull. Canad. Math. Soc. 33 (1990) 139–144. In Contemp. Math., Vol. 253, 2000, pp. 125–144, Henriksen and Smith asked to what extent the result above can be generalized to a larger class of algebras. In the present paper, we give an answer to that question as follows. Let A and B be uniformly closed Φ-algebras. We first prove that every order bounded linear biseparating map from A onto B is automatically a weighted isomorphism, that is, there exist ω in B and a lattice and algebra isomorphism ψ between A and B such that We then assume that every universally σ-complete projection band in A is essentially one-dimensional. Under this extra condition and according to a result from Mem. Amer. Math. Soc. 143 (2000) 679 by Abramovich and Kitover, any linear biseparating map from A onto B is automatically order bounded and, by the above, a weighted isomorphism. It turns out that, indeed, the latter result is a generalization of the aforementioned theorem by Araujo et al. since we also prove that every universally σ-complete projection band in the uniformly closed Φ-algebra C(X) is essentially one-dimensional. 相似文献
(a)=ωψ(a) for all aA.
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<正>Two new eudesmane derivatives were isolated from the leaves and flowers of Verbesina virginica,along with the known 6-O-β-E -p-coumaroyl-4α-hydroxyeudesmane(1).Their structures were determined as 6-O-β-Z-p-coumaroyl-4α-hydroxyeudesmane(2) and 6-O-α-E-p-coumaroyl-1β-4α-dihydroxyeudesmane(3) by spectroscopic methods. 相似文献
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