Carbon-13 nuclear magnetic resonance spectra of anti-inflammatory drugs: Phenylbutazone,oxyphenbutazone and indomethacin

The ¹³C nmr chemical shifts of phenylbutazone, oxyphenbutazone, indomethacin and indole-3-acetic acid are reported. The assignments of various carbon resonances are made on the basis of the substitution effects on benzene shifts, multiplicities generated in SFORD spectra, nuclear overhauser enhancement for protonated carbons, and the comparison with analogous compounds.     

Synthesis and studies of CuHg(NCX)4 (X = S,Se) and their complexes and application of the softness parameter in the elucidation of their structure

Complexes of CuHg(NCS)₄, CuHg(NCS)₂ (NCSe)₂ and CuHg(NCSe)₄ with tetrahydrofuran, dioxane, pyridine, 2-aminopyridine, nicotinamide, bipyridine and phenanthroline have been prepared and comparative studies made. Bipyridine and phenanthroline form cationic—anionic [CuL₃]²⁺ [Hg(SCN)₄]^2? (L = bipy, phen) complexes with CuHg(NCS)₄ and dinuclear bridged complexes with CuHg(NCSe)₄ and CuHg(NCS)₂ (NCSe)₂. For other ligands the nature of the complexes is binuclear or polynuclear. The comparative stability of the -XCN- bridge (X = S, Se) is CuHg(NCSe)₄ > CuHg(NCS)₂ (NCSe)₂ > CuHg(NCS)₄.     

Electron spin resonance spectra of some phosphonyl radicals and related species

The isotropic ESR spectra of a number of phosphonyl radicals (X₂$\text{P}$O), the dimethylphosphinyl radical, and the phosphoranyl radical (MeO)₃$\text{P}$OBu-t, are described, and accurate values of the phosphorus hyperfine splittings and g-factors are reported. For X₂$\text{P}$O, the value of a(P) increases and the g-factor decreases as the electronegativity of X increases. There is a linear relationship between a(P) for X₂$\text{P}$O and ¹J(PH) for X₂P(O)H, but the same relationship does not hold for Me₂P- and Me₂PH. The spectrum of the di-n-hexylphosphonyl radical shows coupling to two pairs of α-methylene protons, and this non-equivalence is attributed to the pyramidal structure of the phosphonyl radical.     

Using IMAP technology to identify kinase inhibitors: comparison with a substrate depletion approach and analysis of the nature of false positives

IMAP is a fluorescence polarisation-based assay method which can be applied to the measurement of protein kinase activity. Using a model serine/threonine kinase we found that IMAP generated a good assay window (Z' > 0.8), was very tolerant of DMSO, and was flexible with respect to sample processing (stopped reactions were stable over a period of several days). Using a set of six low molecular weight inhibitors of the kinase, we found a good correlation between IMAP and scintillation proximity assay (SPA) potency data. IMAP, which measures product accumulation, was compared in an HTS setting with a substrate depletion method (luminescence-based measurement of ATP concentration). There was a reasonable (approximately 50%) overlap in primary hits from a 17,000 compound set, but more apparent false positives were generated from the IMAP method. We followed up the compounds that showed activity in the IMAP method but not in the luminescence assay. Approximately 10% of these compounds displayed intrinsic fluorescence, suggesting that they were false actives by virtue of intrinsic spectroscopic properties. Compound activity by competition of phosphopeptide binding to IMAP beads can occur with high concentrations of chelating compounds, but did not occur with any of the false actives, suggesting that this form of interference is rare.     

Electrochromatography in the separation of ions: Separation of alkaline earth metals

Complete Separation of ternary mixtures of Ca, Mg and Sr or Ba ions with ammonium chloride and less complete but detectable separation of the same mixtures with ammonium nitrate have been achieved, as well as a few binary separations of the type Ca-Ba, Ca-Sr, Ca-Mg, Mg-Sr and Mg-Ba.