An effective electrochemical sensor for the rapid and simultaneous determination of tramadol and acetaminophen based on carbon paste electrode (CPE) modified with NiFe2O4/graphene nanoparticles was developed. The structures of the synthesized NiFe2O4/graphene nanocomposite and the electrode composition were confirmed by X-ray diffraction (XRD) spectrometry, Fourier transform infrared (FT-IR) spectrometry and scanning electron microscopy (SEM). The peak currents of square wave voltammetry of tramadol and acetaminophen increased linearly with their concentration in the range of 0.01–9 μmol L−1. The detection limit for their determination was found to be 0.0036 and 0.0030 μmol L−1, respectively. The results show that the combination of graphene and NiFe2O4 nanoparticles causes a dramatic enhancement in the sensitivity of the sensor. The fabricated sensor exhibited high sensitivity and good stability, and would be valuable for the clinical assay of tramadol and acetaminophen. 相似文献
A novel and highly efficient synthetic method leveraging microwave-assisted organic synthesis (MAOS) to yield di-7-azaindolylmethanes (DAIMs) is reported. Under MAOS conditions, reaction of 7-azaindole with aldehydes resulted predominantly in DAIMs, as opposed to the expected 7-azaindole addition products that form at ambient temperature. Based upon studies of different indoles and azaindoles with various aromatic and aliphatic aldehydes, we herein propose a mechanism where rapid and efficient microwave heating promotes nucleophilicity of 7-azaindoles toward the corresponding alkylidene–azaindolene intermediate to form the DAIM. This sequence provides a versatile approach to efficiently synthesize novel DAIMs that may be useful pharmaceuticals. 相似文献
Activation cross-sections of natCu(d,x)62,65Zn,64Cu,60g+mCo,59Fe reactions were measured using a stacked foil activation technique combined with HPGe gamma-ray spectrometry up to the deuteron energy of 24 MeV. Measured data were critically compared with the available literature data and theoretical data extracted from the TENDL-2012 library, and found only partial agreements among them. Cross-sections of natCu(d,x)59Fe reactions are reported here for the first time in the energy region of 19–23 MeV. Measured cross-sections of the long-lived 65Zn, 60gCo and 59Fe radionuclides are significant for understanding the activation behaviour of copper, an essential structural material used in accelerator and nuclear industry. 相似文献
Chemiluminescence (CL) is an important method for quantification and analysis of various macromolecules. A wide range of CL agents such as luminol, hydrogen peroxide, fluorescein, dioxetanes and derivatives of oxalate, and acridinium dyes are used according to their biological specificity and utility. This review describes the application of luminol chemiluminescence (LCL) in forensic, biomedical, and clinical sciences. LCL is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays. Here, we also provide information about different compounds that may enhance or inhibit the LCL along with the effect of pH and concentration on LCL. This review covers most of the significant information related to the applications of luminol in different fields. 相似文献
Three novel pyrazolo-[4,3-e][1,2,4]triazolopyrimidine derivatives (1, 2, and 3) were designed, synthesized, and evaluated for their in vitro biological activity. All three compounds exhibited different levels of cytotoxicity against cervical and breast cancer cell lines. However, compound 1 showed the best antiproliferative activity against all tested tumor cell lines, including HCC1937 and HeLa cells, which express high levels of wild-type epidermal growth factor receptor (EGFR). Western blot analyses demonstrated that compound 1 inhibited the activation of EGFR, protein kinase B (Akt), and extracellular signal-regulated kinase (Erk)1/2 in breast and cervical cancer cells at concentrations of 7 and 11 µM, respectively. The results from docking experiments with EGFR suggested the binding of compound 1 at the ATP binding site of EGFR. Furthermore, the crystal structure of compound 3 (7-(4-bromophenyl)-9-(pyridin-4-yl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidine) was determined by single crystal X-ray analysis. Our work represents a promising starting point for the development of a new series of compounds targeting EGFR. 相似文献
In this paper, two-grid finite element method for the steady dual-permeability-Stokes fluid flow model is proposed and analyzed. Dual-permeability-Stokes interface system has vast applications in many areas such as hydrocarbon recovery process, especially in hydraulically fractured tight/shale oil/gas reservoirs. Two-grid method is popular and convenient to solve a large multiphysics interface system by decoupling the coupled problem into several subproblems. Herein, the two-grid approach is used to reduce the coding task substantially, which provides computational flexibility without losing the approximate accuracy. Firstly, we solve a global problem through standard Pk ? Pk??1 ? Pk ? Pk finite elements on the coarse grid. After that, a coarse grid solution is applied for the decoupling between the interface terms and the mass exchange terms to solve three independent subproblems on the fine grid. The three independent parallel subproblems are the Stokes equations, the microfracture equations, and the matrix equations, respectively. Four numerical tests are presented to validate the numerical methods and illustrate the features of the dual-permeability-Stokes model.
An L-band remotely-pumped erbium-doped fiber amplifier incorporating a secondary pumping scheme utilizing stimulated Raman
Scattering (SRS) was demonstrated. 1423 nm Raman laser was employed to generate SRS which became the secondary pump source.
The amplifier displayed excellent gain of up to 27.3 dB at 1570 nm for −30 dBm input. Noise figures were also kept to a minimum,
with the highest figure measured at 11 dB which was influenced by imperfection of the C/L coupler utilized in this architecture.
Overall transmission performance was measured as well and demonstrated an encouraging outcome with gain as high as 24 dB while
the noise figure was maintained at about 11 dB. The L-band signal amplification was also contributed by the stimulated Raman
scattering along the transmission fiber. The outcome of this study emphasized the feasibility of secondary pumping scheme
using SRS in L-band gain enhancement. 相似文献
Sound velocity determination in seawater is a key component of modern hydrographic surveying; however, little data exists
on sound velocity characteristics of the southern Caspian Sea. Hence, a study was undertaken in 2008 to examine the seasonal
variability of sound speed in deep-waters of the South Caspian Sea near the Iranian coast. The seasonal cycle of seawater
temperature and thermal stratification in the Caspian Sea water created a wide range of spatial and temporal changes of sound
speed with relevant differences between shallow water (over the continental shelf) and deep-water area. The collected data
showed that seasonal variations of the sound speed were most important in the upper 100 m water depth, while below this level
that is in deepwater the changes were small. The maximum values of sound speed were observed at the surface in midsummer around
1517–1519 m s−1 over the continental shelf while the speed of sound was about 1453 m s−1 between 450–470 m depths with no major seasonal variations. Variations in vertical structure of the sound speed were in agreement
with temperature changes, while effects of the salinity on the sound speed were little. 相似文献
We observed amplified spontaneous emissions (ASEs) in solutions of conjugated polymer under high power neodymium-doped yttrium aluminum garnet (Nd:YAG) laser excitation. A solution of coumarin-485 dye (C485) and conjugated polymer (MEH-PPV) was prepared in tetrahydrofuran (THF). Here, C485 acted as a donor (with different ratios) while MEH-PPV served as an acceptor (with a constant ratio). Without the donor, MEH-PPV exhibited ASE only in the excimeric state; with the donor, however, it produced ASE in both the monomeric and excimeric states simultaneously. These results clearly show that it is possible to monitor and manipulate the excited state behavior of molecules by using an energy transfer process using laser-excited conjugated polymer. 相似文献