Particle removal from a surface by a bounded vortex flow

A bounded vortex flow consists of an axisymmetric vortex that is confined top and bottom between two plates (the “confinement plate” and “impingement plate”, respectively) and surrounded laterally by a swirling annular slot jet. The bottom of the vortex terminates on the boundary layer along the impingement plate and the top of the vortex is drawn into a suction port positioned at the center of the confinement plate. The circumferential flow within the annular jet is important for supplying circulation to the central wall-normal vortex. This flow field is proposed as a method for mitigation of dust build-up on a surface, where the vortex–jet combination supplements the more traditional vacuum port by enhancing the surface shear stress and related particle transport rate. The paper reports on a computational study of the velocity field and particle transport by a bounded vortex flow. Fluid flow computations are performed using a finite-volume approach for an incompressible fluid and particle transport is simulated using a discrete-element method. Computations are performed for different values of two dimensionless parameters – the ratio of the plate separation distance and the average radial location of the jet inlet (the dimensionless confinement height) and the ratio of flow rate withdrawn at the suction outlet and that injected by the jet (the flow rate ratio). For small values of the flow rate ratio, the impinging jet streamlines pass down to the boundary layer along the bottom surface and then travel up the vortex core. By contrast, for large values of flow rate ratio, the annular jet is quickly entrained into the suction outlet and no wall-normal vortex is formed. Particles are observed to roll along the impingement surface in a direction determined by the fluid shear stress lines. Particles roll outward when they lie beyond a separatrix curve of the surface shear stress lines, where particles within this separatrix curve roll inward, piling up at the center of the flow field. A toroidal vortex ring forms for the small confinement height case with flow rate ratio equal to unity, which yields double separatrix curves in the shear stress lines. The inward rolling particles intermittently lift up due to collision forces and burst away from the impingement surface, eventually to become entrained into the flow out the suction port or resettling back onto the impingement surface.     

Protein nanopatterns by oxime bond formation

Patterning proteins on the nanoscale is important for applications in biology and medicine. As feature sizes are reduced, it is critical that immobilization strategies provide site-specific attachment of the biomolecules. In this study, oxime chemistry was exploited to conjugate proteins onto nanometer-sized features. Poly(Boc-aminooxy tetra(ethylene glycol) methacrylate) was synthesized by free radical polymerization. The polymer was patterned onto silicon wafers using an electron beam writer. Trifluoroacetic acid removal of the Boc groups provided the desired aminooxy functionality. In this manner, patterns of concentric squares and contiguous bowtie shapes were fabricated with 150-170-nm wide features. Ubiquitin modified at the N-terminus with an α-ketoamide group and N(ε)-levulinyl lysine-modified bovine serum albumin were subsequently conjugated to the polymer nanopatterns. Protein immobilization was confirmed by fluorescence microscopy. Control studies on protected surfaces and using proteins presaturated with O-methoxyamine indicated that attachment occurred via oxime bond formation.     

Synthesis of Aminooxy End-functionalized pNIPAAm by RAFT Polymerization for Protein and Polysaccharide Conjugation

A Boc-protected aminooxy end-functionalized poly(N-isopropylacrylamide) (pNIPAAm) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. The monomer was polymerized in the presence of a Boc-protected aminooxy trithiocarbonate chain transfer agent (CTA) utilizing 2,2'-azobis(2-isobutyronitrile) (AIBN) as the initiator in DMF at 70 °C. The final polymer had a number-average molecular weight (M(n)) of 4,200 Da as determined by (1)H NMR spectroscopy and a narrow polydispersity index (1.14) by gel permeation chromatography (GPC). The Boc group was removed, and the polymer was then incubated with N(ε)-levulinyl lysine-modified bovine serum albumin (BSA). Gel electrophoresis confirmed that the conjugation was successful. The aminooxy end-functionalized pNIPAAm was also immobilized on a gold surface after reduction of the trithiocarbonate end-group. The pNIPAAm surface was then incubated with an aldehyde-modified heparin to yield the polysaccharide-functionalized surface. All surface modifications were monitored by FT-IR spectroscopy.     

An ab initio mo study of calcium and magnesium complexes with malonate and formate

Quantum mechanical SCF calculations were performed on magnesium and calcium complexes of malonate and malonate/formate coordination. The calcium malonate structure is in substantial agreement with recent X-ray results on -ethylmalonate. The magnesium complexes are generally more tightly bound than calcium, although qualitatively, the calcium and magnesium structures are similar. Experimental binding results for divalent ions with malonates and EDTA are discussed in view of the current calculations.     

Measurements of elastic constants in thin films of colossal magnetoresistance material

Measurements of elastic constants of strained 200 and 400 nm thin films, as well as unstrained samples, of the colossal magnetoresistance (CMR) material La0.67Ca0.33MnO3 are presented. Since the peak resistance temperature of a strained CMR film decreases as the film thickness decreases, it is of interest to see if features in the elastic constants, reflecting structural or magnetic changes, follow the peak resistance temperature. It is observed that features in the elastic constants appear not only at the peak resistance temperatures of the CMR samples, but also at a temperature about 17 K higher. A new technique, thin-film resonant ultrasound spectroscopy, was used to make the measurements.     

Thermoelectric power of electron-irradiated graphite at low temperatures

The thermoelectric power (TEP) of very well oriented, nearly single-crystal graphite irradiated at 333 K by 3 MeV electrons was measured along the basal plane direction above 5 K. Changes in the low-temperature dependence of the TEP after different stages of annealing, at 410 and 520 K, have also been investigated. Analysing carefully the experimental data, the phonondrag component S_ph of the TEP is extracted and compared with the theory of Jay-Gerin and Maynard. As a check of the concentration of the irradiation defects, deduced from this analysis, measurements of the thermal conductivity were made on the same samples. The results are found to be in good agreement.     

Numerical continuation of degenerate homoclinic orbits in planar systems

In this paper we develop numerical algorithms for the continuationof degenerate homoclinic connections in planar systems. We considerthe case where the equilibrium point has zero trace and twocases of higher-order degeneracies. The method we propose isable to continue homoclinic connections of order up to codimension-four.Application of the algorithm to four examples supports its validityand demonstrates its usefulness.     

Gas-phase deprotonation of arylalkylamines. A collision-induced dissociation study

The collision-induced dissociation (CID) of deprotonated arylalkylamines of general formula R(1)C(6)H(4)CHR(2)CH(2)NR(3)(2) (where R(1) = H, OH, F or NO(2); R(2) = H or OH; R(3) = H or CH(3)) generated by negative chemical ionization with H(2)O and D(2)O as ionizing reagents, is discussed. The negative chemical ionization mass spectra show that, in the absence of a hydroxy group in the aromatic ring, deprotonation takes place at the benzylic position whereas the proton is lost from the OH group when present. The nitro compound forms only M(-.) ions. The CID spectra of the deprotonated molecules show that fragmentations are strongly dependent on the structural features of the molecules, namely the presence or absence of substituents in the aromatic ring or aliphatic chain. Copyright 1999 John Wiley & Sons, Ltd.